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Showing 1 to 20 of 30 for “"real-time quantitative PCR"”.

  1. Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome

    Jackson, Tamra A.University of Illinois at Urbana-Champaign2015

    … was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only …

    uiuc Repository record

  2. The effect of phosphorus and nitrogen limitation on viral infection in microcystis aeruginosa NIES298 using the cyanophage Ma-LMM01

    McKindles, Katelyn2018

    <p>Throughout different times in freshwater cyanobacterial harmful algal blooms (cHAB), the availability of specific nutrients from the environment varies, causing fluctuations in the severity and species composition of a bloom. In addition to nutrient regulation of blooms, the cyanobacteria are …

    emich Repository record

  3. The rumen microbial ecosystem during the transition period in dairy cows

    Alqarni, SalehUniversity of Illinois at Urbana-Champaign2014

    … haylage. Ruminal digesta were collected at five times: -14, -7, 10, 20, and 28 days around parturition. Total DNA from ruminal digesta was isolated and real-time quantitative PCR was used to determine the relative abundance of bacterial species. Eubacterium ruminantium and Selenomonas ruminantium …

    uiuc Repository record

  4. Quantitative analysis and characterization of intracellular gene delivery mechanisms

    Varga, Csanad M. (Csanad Mathias), 1976-Massachusetts Institute of Technology2003

    … and potential rate limiting steps unidentified. Quantitative analysis of the gene delivery pathway is essential for the characterization, comparison, and design of vectors. The complex nature of the mechanisms for gene delivery, particularly at the cellular level, contains multiple potentially …

    mit Repository record

  5. Vimentin Overexpression Contributes To the Biological Properties of Metastatic Head and Neck Cancer Cells

    Paccione, Rachel J.2014

    … this study, we confirmed this difference using real-time quantitative PCR, western blot analysis, and immunostaining. Furthermore, EGF and TGF-β, growth factors that induce migration and invasion of HN12 cells, produced synergistic increases in vimentin expression. To assess the contribution of …

    vcu Repository record

  6. Effect of Chinese Knotweed Extract on HSV-1 Infection of Vero Cells

    Prince, Derek J.2015

    … decreased. Furthermore, molecular analysis from real-time quantitative PCR (qPCR) assays suggest that 0.1% CKs concentrations are able to inhibit >98% of HSV-1 viral entry when compared to untreated HSV-1 positive controls. Overall, CKs ability to prevent in vitro HSV-1 infection may provide …

    shu-thes Repository record

  7. Enhancement of Protective Immune Responses in Chickens against Marek's Disease Virus

    Parvizi, PayvandUniversity of Guelph2011

    The work presented in this thesis is an investigation of the use of toll-like receptor (TLR) agonists to induce or enhance immunity in chickens against Marek’s disease virus (MDV). Initially, synthetic double-stranded (ds) RNA, polyriboinosinic polyribocytidylic (poly(I:C)) was administered via …

    guelph Repository record

  8. Regulatory mechanisms involved in the control of <i>CYP6M2</i> gene in insecticide resistant <i>Anopheles gambiae</i> (Diptera: culicidae)

    Mohammed Rabiu, BalarabeAbertay University2014

    … gambiae</i> and cDNA synthesised. Semi and Real time quantitative PCR (qPCR) using SYBR® Green were used to determine the gene expression and regulation levels. Results established the presence of putative AGAP010259 (A<i>h</i>R) and AGAP005300 (Nf2e1) <i>cis</i>-acting elements within …

    abertay Repository record

  9. A Study of Antibiotic Resistance Plasmids in Fresh Produce and In Wastewater Environments Using Functional Approaches, Comparative Genomics and Abundance Analysis

    Ajayi, Adeyinka OlusegunFaculty of Graduate Studies and Research, University of Regina2019

    … pPIMR and pFEMG. Using enrichment culture and real-time quantitative PCR approaches respectively, it was determined that conventional wastewater treatment processes markedly reduced the abundance of plasmids encoding ciprofloxacin resistance genes and incF family incompatibility genes. …

    regina Repository record

  10. Immunotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Diethylstilbestrol (DES) in the Fetal Mouse Thymus and Liver

    Besteman, Elizabeth GayleVirginia Tech2006

    … basophilia and more prominent nucleoli. Real time quantitative PCR demonstrated that DES decreased c-jun, Bcl-2, and PKCalpha mRNA expression. These results suggest a shift away from proliferative activity and may reflect alterations noted predominantly in the hematopoietic population. …

    vt Repository record

  11. Human Adenoviruses As Indicators Of Faecal Contamination And Pathogen Indices In The Environment And Shellfish

    Hewitt, JoanneResearchSpace@Auckland2015

    … for virus concentration from water samples; real-time quantitative PCR (qPCR) assay development; evaluation of culture-qPCR using different cell lines; and assessment of guanidine hydrochloride to inhibit enterovirus growth in virus quantification assays. The presence, quantity, and where …

    auckland-ms Repository record

  12. Genetic aspects of outcome in kidney transplantation : cytokine and thrombosis associated candidate genes and gene expression biomarkers

    Alakulppi, NooraHelsingin yliopisto2008

    … collected from adult kidney Tx patients. With real-time quantitative PCR (RT-QPCR) gene expression quantities of CD154 and ICOS differentiated the patients with AR from those without, but not from the patients with other causes of graft dysfunction. Biomarkers for SCR were studied in paediatric …

    helsinki Repository record

  13. Zusammenhang zwischen methanogenen Archaea und sulfatreduzierenden Mikroorganismen bei Parodontitis

    Holtgräwe, Simone MareenPublikationsserver der RWTH Aachen University2009

    … disease and analyzed with the help of real time quantitative PCR (polymerase chain reaction). They were compared to samples taken from a control group of 65 healthy subjects. Functional genes specifically for methanogenic archaea and for dissimilatory sulfate-reducers allowing the …

    aachen Repository record

  14. Aberrations of A Putative Tumor Suppressor Gene Sel1L In Pancreatic Ductal Adenocarcinoma

    Liu, Qian2012

    … transcription polymerase chain reaction (RT-PCR), and analyzed by agarose gel electrophoresis and sequenced to search for mutations. Using fluorescent fragment analysis, two intragenic microsatellites in the <em>SEL1L</em> gene region were examined to detect LOH in a total of 73 pairs of PDAC …

    uthsc Repository record

  15. Differential effects of progestogens on HIV-1 replication and host gene expression in primary PBMCs and cervical tissue explants

    Ray, Roslyn MichelleDepartment of Molecular and Cell Biology2015

    … blood mononuclear cells (PBMCs) from women, by real time quantitative PCR, western blotting and Luminex assays, in response to physiologically relevant doses of progestogens. Infection assays were performed in the absence and presence of HIV-1using HIV-1BAL-RENILLA or HIVpNL4.3 infectious …

    cape-town Repository record

  16. Investigation of the Genetic Basis of Antibiotic Resistance in Mycobacterium tuberculosis

    Evans, JoannaDivision of Medical Microbiology2010

    … of Mycobacterium tuberculosis, coupled with the time it takes to perform phenotypic drug susceptibility testing of this organism, makes the treatment of tuberculosis increasingly difficult. Several genotypic assays for the rapid detection of drug resistance in M. tuberculosis have been developed, …

    cape-town Repository record

  17. Growth hormone and somatolactin function during sexual maturation of female Atlantic salmon

    Benedet, Susana2008

    … tools for analysis of mRNA levels using real time quantitative PCR (RTqPCR). The roles of GH, IGF-I and SL were examined in a 17-month long study on one sea winter Atlantic salmon females. mRNA expression levels of ovarian components of the GH-IGF-I system and SLR and pituitary GH, SLa …

    goteborg Repository record

  18. Molecular analysis of La Crosse virus transcription and replication in host cells

    Kempf, Brian Joseph, author, Beaty, Barry J., advisor, Blair, Carol D., advisor, Miller, Barry, committee member, Roberts, Elaine, committee member, Olson, Kenneth, committee memberColorado State University. Libraries2003

    … studies utilizing a reverse transcription-PCR (RT-PCR) based assay examined co-regulation of LAC virus RNA synthesis with host metabolic activity. A more sensitive real-time quantitative PCR (Q-PCR) based assay was developed during this study to detect and quantitate the LAC virus …

    colostate Repository record

  19. Thyroid hormone action and disruption in the brain: Xenopus as a model to study disruption of thyroid hormone availability on early brain development

    Mughal, Bilal BabarMNHN/Sorbonne University2017

    … mct8, showed active efflux, but no influx. Using real-time quantitative PCR (RT-qPCR) and in situ hybridization, specific expression of the mct8 was observed in the brain, with increasingly strong expression as development progressed. Using the before mentioned in vitro assay, effects of common …

    liege Repository record

  20. Role of peritoneal mesothelial cells and the inflammatory response in peritoneal fibrosis

    Wu, XuanThe University of Edinburgh2011

    … and large peritoneal wounds heal in a similar time. The peritoneum is a monolayer of elongated, flattened, squamous-like peritoneal mesothelial cells (PMC). Local mesothelial cell proliferation, centripetal cell migration from the wound edge, as well as incorporation of free-floating …

    edinburgh Repository record

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