Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"real-time qPCR"”.
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Native chromatin extraction and its structure analysis
… transcribed as cDNA and further characterized by real-time qPCR. The total assay time taken for cell separation, chromatin purification and chromatin associated RNAs extraction can be accomplished in less than 2h. Finally, we further applied cryogenic electron microscopy to analyze higher order …
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Variations of Vibrio Populations in Pacific White Shrimp, <i>Litopenaeus vannamei</i>, Aquaculture Raceways
… to maintaining yields. This study performed real-time qPCR (quantitative polymerase chain reaction) using primers targeting 16s rDNA sequences for total bacteria, the <em>Vibrio</em> genus, and <em>Vibrio parahaemolyticus</em> in aquaculture tanks over the growing season. Total DNA …
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Defining the molecular, genetic and transcriptomic mechanisms underlying the variation in glycation gap between individuals
… in genotype frequency of specific SNPs via real time qPCR,determine soluble receptors for AGE (sRAGE) concentration via ELISA, finding association of sRAGE concentration with SNPs genotype, and evaluate relative average telomere length ratio via real time qPCR. This study also aimed at the …
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An in vitro cell lineage model to identify alterations in the differentiation program of neuroblastoma
… cells, SGNs and ChCs cells. Marker analysis by real-time qPCR, immunofluorescence, ELISA assays and functional characterization by multi- electrode array recording (MEA), confirmed the effectiveness of our differentiation protocols. The resulting differentiated cells will be used to perform bulk …
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Modulation of the progesterone receptor by progestogens, antiretroviral drugs and the glucocorticoid receptor
… investigated on endogenous genes by quantitative real-time PCR (real-time qPCR) in the MDA-MB-231 PR-B-stably expressing (MDA-PR-B+) cells. Once the progestogen responses via the PR were characterised, PR-B activity in the presence of three ARVs namely; tenofovir disoproxil fumarate (TDF), …
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A dynamic transcriptome technique for transcriptional profiling and gene regulatory network involving the helicase antigen (HAGE)
… altered during tumorigenesis. Confirmation using real-time qPCR suggested change in expression of certain genes could be recognised in other HAGE-expressing tumour cell lines. This analysis also indicated a possible interaction between HAGE and the oncogene N-RAS. Subsequent genetic and protein …
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Linking transcript, QTL and association mapping to understand the genetic control of leaf size and shape in Populus
… years and biomass estimated at one point in time. Results indicate that leaf traits vary with latitude of sample origin, with significant differences observed in leaf area, epidermal cell number and biomass, but not in leaf shape (leaf ratio), epidermal cell area, stomatal density and …
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The use of molecular diagnostic methods to improve the detection of the common bacterial and viral causes of community acquired meningitis in children in South Africa
… We developed and validated in-house quantitative real-time (qPCR) multiplex assays to test for bacterial causes namely: Neisseria meningitidis (ctrA gene), Haemophilus influenzae (hpd gene) and Streptococcus pneumoniae (lytA gene) and viral causes namely: enterovirus (5' UTR), herpes simplex (UL30 …
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Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction
… infection of soybean roots by H. glycines with real-time quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a consistent inoculation method was developed using to provide active …
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INSIGHTS ON THE CROSSTALK BETWEEN THE IMMUNE SYSTEMAND CANCER: IMMUNE CHECKPOINT MOLECULES AND LYMPHOIDPOPULATIONS ASSESSMENT IN CANINE ROUND CELL TUMORS
… by means of flow cytometry (FC), mRNA by real-time qPCR, and soluble protein (sPD-L1) plasmatic concentrations by competitive ELISA in dogs with nodal lymphoma. Their potential prognostic role was also investigated. B-cell lymphomas showed the highest frequency and level of mPD-L1 …
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Investigation of pathogens removal from wastewater using a hydroponic system
… were collected at different hydraulic retention times and analyzed for physiochemical parameters (temperature, pH and dissolved oxygen), organic material and pathogens concentrations. A field multimeter probe was used for measuring physiochemical parameters. The respective cell tests and …
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DENVax live attenuated chimeric dengue vaccine
… which was developed by passaging the virus 53 times in primary dog kidney cells (PDK). The attenuating mutations in the dengue-2 PDK-53, which are in the nonstructural genes of the virus, are well defined and characterized. This is essential in a live attenuated vaccine in order to monitor the …
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Molecular and biochemical characterisation of esterases from oenococcus oeni and their potential for application in wine.
… strain specific differences in esterase activity real-time qPCR was carried out on an independent set of samples (n = 30) using strain Ooeni28 (‘high’ activity against pNP-linked ester substrates). Expression of esterase genes was measured in the presence of known esterase substrates. Both estA2 …
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SVILUPPO DI TECNICHE PER L'ISOLAMENTO, LA CARATTERIZZAZIONE E IL DIFFERENZIAMENTO DI CELLULE STAMINALI MESENCHIMALI PER UN USO IN MEDICINA RIGENERATIVA
… a day by day gene profiling analisis by Real-Time qPCR. This detailed study informed us on the regulation of the genes during the different phases, early, intermediate and late, of the osteogenic process. It gave us a more complete vision compare to what you can find in the literature.
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The role of mitochondria in regulating smooth muscle cell proliferation and migration in pulmonary hypertension
… and genes were measured by Western blot and real-time qPCR techniques, respectively. Mitochondrial functions were assessed by measuring ATP level, reactive oxygen species (ROS; superoxide and H2O2) production, and cellular apoptosis. Mitochondrial morphology was examined using an …
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Photodynamic therapy against multidrug-resistant oral strains of Candida spp
… C. albicans. From culture medium, at different times, total RNA was extracted, and MiRNAs were quantified by real-time qPCR protocol. In vivo clinical study. A set of 12 patients with refractory Candidiasis have been treated with commercial PDT protocols, thus a clinical follow up was performed …
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Mechanisms of genomic stability maintenance in fission yeast
… origins is activated in order to achieve timely duplication of the genome in the timeframe of S. The subset of origins activated in each cell cycle constitute the replication program of the cell. Despite the fact that origin selection is characterized by stochasticity, there are several …
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Assessment of knowledge, attitude and practices of small household farmers towards heartwater disease and molecular characterization of Ehrlichia ruminantium in Limpopo province, South Africa
… was extracted from the ticks and analysed using real-time qPCR assay targeting a 226bp fragment of the PCS20 gene. Finally, a number of samples were sent for sequencing to identify different strains circulating in the region. Neighbor-joining method was used to infer phylogenetic positions on the …
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THE ROLE OF NOTCH SIGNALING IN THE DIFFERENTIAL ABILITY OF SUPPORTING CELL SUBTYPES TO SPONTANEOUSLY REGENERATE HAIR CELLS IN THE NEONATAL MOUSE COCHLEA
… genes in the Notch pathway were measured using real time qPCR, immunostaining, and in situ hybridization. The Notch effector HeyL was increased in the apical one-third of the cochlea while other Notch players are decreased. The most notable example is the Notch effector Hes5, which is directly …
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Gas-phase biofiltration for livestock building ammonia emission mitigation
… to the moisture changes. Interestingly, the real-time qPCR results showed that the quantity of nosZ gene copy was reduced significantly at 63% moisture content. This observation can explain the increasing of nitrous oxide concentrations in this step. Based on the previous study results of …
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