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Showing 1 to 20 of 22 for “"real-time polymerase chain reaction (PCR)"”.

  1. Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes

    Booley, GhowaDepartment of Clinical Laboratory Sciences2022

    … evaluated the utility of a previously published real-time PCR assay for detecting Bartonella spp. on cats. This will be the first time this target will be evaluated in a real-time PCR assay to detect Bartonella spp. in human samples. For this study, we constructed a plasmid vector containing an …

    cape-town Repository record

  2. The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study

    이, 현수2015

    … fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad …

    ajou Repository record

  3. MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS

    Pierpont, Melissa J.2016

    … facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>

    nmu Repository record

  4. Development of tissue printed nitrocellulose cards/arrays for real time PCR amplification and detection

    Chia, Helena Nien-Hwa, 1982-Massachusetts Institute of Technology2004

    … in bio-medicine by combining it with imaging and real time polymerase chain reaction (PCR) amplification and detection. A major objective in the design of this instrumentation is to develop the capacity to evaluate much larger areas of tissue. An approach to fulfilling this objective is the …

    mit Repository record

  5. Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice

    정, 종갑2016

    … analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs 13 6. Uptake of 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino-2-deoxyglucose (2-NBDG) 14 7. Animal experiments 14 8. FGF21 secretion 15 9. IL-1&beta; assay 15 10. Statistical analysis 15 Ⅲ. …

    ajou Repository record

  6. Effect of Poor Sanitation Procedures on Cross-Contamination of Animal Species in Ground Meat Products

    Chung, Sunjung2021

    … and each sanitation treatment was tested twice. Real-time polymerase chain reaction (PCR) was used to quantify pork in ground beef. For “no cleaning,” the first 100-g sample of ground beef run through the grinder contained 24.42 ± 10.41% pork, while subsequent samples contained</p>

    chapman Repository record

  7. Real time PCR measurement by fluorescence anisotropy

    Crane, Bryan Lee, 1976-Massachusetts Institute of Technology2005

    Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues …

    mit Repository record

  8. Identification of Species in Ground Meat Products Sold on the U.S. Commercial Market Using DNA-Based Methods

    Kane, Dawn

    … mixture, these samples were also tested with real-time polymerase chain reaction (PCR) targeting beef, chicken, lamb, turkey, pork and horse. Of the 48 products analyzed in this study, 10 were found to be mislabeled, with nine containing multiple meat species. Meat samples purchased from …

    chapman Repository record

  9. Mechanical Strain-Mediated Syndecan Regulation and Its Effects on Adhesion of Vascular Smooth Muscle Cells

    Julien, Mathéau A.Georgia Institute of Technology2005

    … by mechanical strain of SMCs. Using real-time polymerase chain reaction (PCR), the kinetics of both static and cyclic mechanical strain were found to modify the gene expression in a time and strain magnitude-dependent manner unique to each syndecan. In particular, the responses of …

    gatech Repository record

  10. The Detection and Role of Human Endogenous Retrovirus K (HML-2) In Rheumatoid Arthritis

    Freimanis, Graham L.University of Wolverhampton2008

    … and disease controls. Additionally, a novel real-time Polymerase Chain Reaction (PCR) assay was developed to accurately quantify levels of HERV-K (HML-2) gag expression, relative to normalised levels of housekeeping gene expression. Both serological and molecular assays showed significant …

    wlv Repository record

  11. Plasma Volume and Albumin mRNA Expression in Exercise Trained Rats

    Bexfield, Nathan Alex2007

    … HI group. Albumin mRNA expression determined by real time polymerase chain reaction (PCR) increased 2.2 ± 0.1 and 2.9 ± 0.2 fold following LI and HI exercise training, respectively. Conclusion- These data support the hypothesis that, during exercise-induced PV expansion, albumin gene expression …

    byu Repository record

  12. Functional genes and gene array analysis as tools for monitoring hydrocarbon biodegradation

    Nyyssönen, MariHelsingin yliopisto2009

    … intensities to hydrocarbon mineralization, real-time polymerase chain reaction (PCR), dot blot hybridization and both chemical and microbiological monitoring data. The results obtained by real-time PCR, dot blot hybridization and gene array analysis were in good agreement with hydrocarbon …

    helsinki Repository record

  13. The Influence of Growth Stage on Carcass Composition and Factors Associated with Marbling Development in Beef Cattle

    Kern, Simon Alexander2017

    … within 30 m postmortem and snap frozen for real time polymerase chain reaction (PCR) and western blot analysis of lipoprotein lipase, AMP activated protein kinase α (AMPKα), stearoylcoenzyme A desaturase (SCD), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding …

    sdstate Repository record

  14. Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry

    Everley, Robert A.2014

    Liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOF MS) utilizing electrospray ionization was employed to monitor protein expression in Escherichia coli and Shigella organisms. Automated charge state deconvolution, spectral subtraction and spectral mirroring were used to …

    vcu Repository record

  15. MicroRNA as Regulator of the Th17 Inflammation in Behcet's disease

    나, 소영2013

    … enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) with or without Th17 differentiation condition. The expression of miR-155 was also investigated using real-time PCR in PBMCs and isolated CD4+ T cells. …

    ajou Repository record

  16. The development, implementation and evaluation of a real-time PCR-based diagnostic service for viral causes of infectious intestinal disease

    Gunson, Rory N.University of Glasgow2008

    … issue resulting in significant morbidity and sometimes mortality each year. The economic costs associated are substantial. Laboratory diagnosis of viral IID is important as the many infectious and non-infectious causes cannot be reliably differentiated using clinical or epidemiological …

    glasgow Repository record

  17. Estudio de la influencia de los polimorfismos genéticos en la respuesta a tratamiento con ranibizumab en la degeneración macular asociada a la edad.

    Miranda Rollón, María Dolores2017

    … de reacción en cadena de la polimerasa a tiempo real (Real Time Polymerase Chain Reaction, PCR) usando sondas KASPar SNP Genotyping System (LGC, Middlesex, UK). Resultados: Encontramos diferencias estadísticamente significativas entre los polimorfismos TGFBR1 (rs334353), COL8A1L/FILIP1L …

    murcia-diss Repository record

  18. Improvement of asexual propagation and Anisogramma anomala detection protocols to facilitate applied breeding of new Corylus avellana and C. americana x C. avellana germplasm

    Revord, Ronald ScottUniversity of Illinois at Urbana-Champaign2016

    … of eight years from seed to seed (generation time) and an additional nine years for selected individuals to proceed through replicated performance trials to cultivar release. At present, layering techniques are the primary method for asexual propagation of Corylus selections during the …

    uiuc Repository record

  19. ELUCIDATING THE ROLE OF HET0016 IN AMELIORATING 20-HETE INDUCED OXIDATIVE STRESS AND LIVER INJURY IN B-THALASSEMIA: A NOVEL THERAPEUTIC TARGET

    BOU FAKHREDIN, RAYANUniversità degli Studi di Milano2025

    … protein levels, were measured in the liver using Real-time Polymerase Chain Reaction (PCR) and western blot, respectively. Degree of expression of CYP4A was also evaluated in the liver by immunohistochemistry (IHC). Formalin-fixed paraffin-embedded liver tissues were also sectioned and stained …

    milano Repository record

  20. Investigation on the Molecular Mechanism of Photorejuvenation in Photodynamic Therapy

    김, 수경2014

    … collagen type Iα in the FB were determined by real-time polymerase chain reaction (PCR). The melanin content and dopa oxidase activity of the MC were also evaluated. RESULTS: After stimulating FB with KC-conditioned medium, the mRNA of MMP-1 was decreased and the mRNA of collagen type Iα were …

    ajou Repository record

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