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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 93 for “"real-time polymerase chain reaction"”.

  1. Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes

    Booley, GhowaDepartment of Clinical Laboratory Sciences2022

    … evaluated the utility of a previously published real-time PCR assay for detecting Bartonella spp. on cats. This will be the first time this target will be evaluated in a real-time PCR assay to detect Bartonella spp. in human samples. For this study, we constructed a plasmid vector containing an …

    cape-town Repository record

  2. Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens

    Price, BrendonDepartment of Pathology2019

    … cases and identify hrHPV genotypes by multiplex real time PCR (RT-PCR). Materials and methods: A retrospective search identified a total of 57 cases of cervical squamous cell carcinoma for the period 2004-2014. This was reduced to a final number of 23 that exhibited sufficient tumour burden for …

    cape-town Repository record

  3. The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study

    이, 현수2015

    … fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad …

    ajou Repository record

  4. MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS

    Pierpont, Melissa J.2016

    … facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>

    nmu Repository record

  5. Development of tissue printed nitrocellulose cards/arrays for real time PCR amplification and detection

    Chia, Helena Nien-Hwa, 1982-Massachusetts Institute of Technology2004

    … in bio-medicine by combining it with imaging and real time polymerase chain reaction (PCR) amplification and detection. A major objective in the design of this instrumentation is to develop the capacity to evaluate much larger areas of tissue. An approach to fulfilling this objective is the …

    mit Repository record

  6. Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice

    정, 종갑2016

    … analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs 13 6. Uptake of 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino-2-deoxyglucose (2-NBDG) 14 7. Animal experiments 14 8. FGF21 secretion 15 9. IL-1&beta; assay 15 10. Statistical analysis 15 Ⅲ. …

    ajou Repository record

  7. The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor

    James, Kris MThe University of Western Ontario2013

    … hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or …

    uwo Repository record

  8. Pathophysiological changes in cardiomyocyte metabolism and bioenergetics during stress-induced cardiomyopathy.

    Fišerová, IvanaUniverzita Karlova, 3. lékařská fakulta2023

    … dose of isoprenaline and chronic hypoxia. Real-time cell metabolic analysis, total lipid analysis, high-performance liquid chromatography (HPLC) and mass spectrometry for lipidomic spectrum detection, real-time polymerase chain reaction (RT-PCR) and Western blot for relative expression of …

    charles-prague Repository record

  9. Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells

    Fang, Siteng

    … cell differentiation period tested. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated the scaffolds developed preferentially support iPSCs to differentiate into mesoderm. Taken together, the study has developed collagen-based scaffolds that support iPSC seeding, …

    binghamton Repository record

  10. Effect of Poor Sanitation Procedures on Cross-Contamination of Animal Species in Ground Meat Products

    Chung, Sunjung2021

    … and each sanitation treatment was tested twice. Real-time polymerase chain reaction (PCR) was used to quantify pork in ground beef. For “no cleaning,” the first 100-g sample of ground beef run through the grinder contained 24.42 ± 10.41% pork, while subsequent samples contained</p>

    chapman Repository record

  11. Biomarkers of Human Exposure and Effect to Petrogenic Polycyclic Aromatic Hydrocarbons

    Yap, Dianne Aster YunqueThe University of Texas Medical Branch at Galveston2021

    … Generation Sequencing (NGS) and quantitative Real Time – Polymerase Chain Reaction (qRT-PCR) were used to identify and verify differentially expressed circulating plasma microRNAs that may serve as novel minimally invasive biomarkers of effect following exposures to bioactive PAHs including …

    utmb Repository record

  12. Characterization of downstream target genes regulated by ABF-1 in different states of B cell development

    Eusebio, Anthony R.2005

    … as the plasma cell line, ARH-77. Quantitative real-time polymerase chain reaction and DNA microarray technology was used to investigate target genes that are subjected to transcriptional regulation by ABF-1. Using ABF-1 inducible cell lines or B cell lines that overexpress ABF -1 by transient …

    u-pacific Repository record

  13. Partial nitrification and oxygen transfer analysis utilizing hollow fiber membrane aeration

    Cotter, Samuel2010

    … to 250 mg/L NH<sub>4</sub><sup>+</sup>–N.y Real–time polymerase chain reaction (qPCR) was employed to verify the system preference for ammonium oxidizing bacteria.y Hollow fiber membrane aeration is effective forcontrolling oxygen transfer.</p>

    iastate Repository record

  14. Phakopsora pachyrhizi growth in tissue of leaves from resistant and susceptible soybean germplasm

    Lu, FanglinUniversity of Illinois at Urbana-Champaign2013

    … more sporulating uredinia indicate a susceptible reaction. Resistance to soybean rust is characterized by the development of reddish-brown (RB) lesions or a symptomless immune response, though considerable variation in lesion diameter, color and sporulation has been observed among resistant …

    uiuc Repository record

  15. Gene Expression in Endometrial Tissues of Normal Mares and Mares With Delayed Uterine Clearance

    Gray, Giles AnthonyVirginia Tech2006

    … the uterine lumen following breeding events, venereal disease or an estral cycle. This syndrome is typically seen in older, multiparous mares and mares with poor reproductive tract conformation. The etiopathogenesis of DUC has not been fully elucidated but suggested causes include poor genital …

    vt Repository record

  16. Evaluation of storage conditions on DNA used for forensic STR analysis

    Beach, Lisa Renae2014

    … method that maintained DNA integrity over time. Four variables were evaluated: storage buffer, storage temperature, initial storage concentration and the effects of repeated freeze-thaw cycles. DNA quantity was assessed using real-time polymerase chain reaction and DNA quality was evaluated …

    iupui Repository record

  17. Genotoxic effects in human peripheral lymphocytes from healthy individuals and head and neck cancer patients after treatment with hydrogen peroxide and pembrolizumab liposome

    Bobtina, Nagah M.A.University of Bradford2022

    … and micronucleus assays. Western blotting and real-time polymerase chain reaction were performed to assess the potential of improving the repair mechanisms after treatment with naked Pembrolizumab and Pembrolizumab liposome. According to the results, Comet assay and micronucleus assay showed a …

    bradford Repository record

  18. Real time PCR measurement by fluorescence anisotropy

    Crane, Bryan Lee, 1976-Massachusetts Institute of Technology2005

    Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues …

    mit Repository record

  19. Anode Material Testing for Marine Sediment Microbial Fuel Cells

    Quaid, Andrew John2018

    … senor systems, for increase deployment times, and reduce the quantity of batteries needed for these systems. This study has two components, firstly, the comparison of power production from anode material with differing surface area and porosity properties, and secondly, to detect and …

    usm Repository record

  20. Molecular genetics of arrhythmogenic right ventricular and dilated cardiomyopathy in South Africans

    Mbele, MzwandileDepartment of Medicine2014

    … markers; for total gene expression we used real time polymerase chain reaction and allelic expression imbalance and exome sequencing was used for mutation screening in two siblings with severe early onset ARVC. To all novel variants identified prediction tools were used to predict the …

    cape-town Repository record

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