Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 467 for “"real time PCR"”.
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Real time PCR measurement by fluorescence anisotropy
Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues …
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Quantitative Real-Time PCR Analysis of Colchicine Pathway and Rhizome Developmental Genes
… development, respectively. Using quantitative real-time polymerase chain reaction (qRT-PCR), a comparative analysis of gene expression in G. superba may reveal differential gene expression of selected candidate genes. Reference genes were selected and analyzed for normalization of qRT-PCR data. …
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Optimization of BRAF V600 Assay as A 2-Step Real-time PCR Protocol
… BRAF Codon Specific Mutation Detection Kit is a real-time PCR assay for the detection of somatic mutations in codon 600 Valine at exon 15 in the BRAF gene which encodes the serine/threonine protein kinase, using purified DNA. The V600E mutation is the most common BRAF gene mutation found in human …
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Etablierung von Real-Time-PCR-Verfahren für die Diagnostik des Cytomegalovirus bei organtransplantierten Patienten
… Rahmen dieser Arbeit wurden quantitative TaqMan-PCR-Verfahren etabliert. Die diagnostische Wertigkeit wurde an 80 PBL-Proben von Organtransplantierten (Niere, Niere/Pankreas, Leber) überprüft und mit Ergebnissen des pp65-Antigentests sowie dem klinischen Verlauf der HCMV-Infektion korreliert. …
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Development of tissue printed nitrocellulose cards/arrays for real time PCR amplification and detection
… in bio-medicine by combining it with imaging and real time polymerase chain reaction (PCR) amplification and detection. A major objective in the design of this instrumentation is to develop the capacity to evaluate much larger areas of tissue. An approach to fulfilling this objective is the …
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The utility of a real-time PCR to detect Leptospira in a routine diagnostic setting
… To address this gap, we developed an inhouse PCR assay targeting the microbe's rrs and lipL32 genes, using primer sets previously reported in literature to be both sensitive and specific for pathogenic Leptospira spp. Using inhouse constructed plasmids, we did a non-clinical, technical …
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Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum
… conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. botulinum types A, B and E was developed. The method was used to diagnose the first case of wound botulism in an injecting drug user in Sweden, which was also the first …
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Subtelomere Chromosomenveränderungen mittels quantitativer Real-Time PCR bei Patienten mit mentaler Retardierung und normalem zytogenetischem Chromosomensatz
… die Vorstellung des mittels der quantitativen Real-Time-PCR am Institut für Humangenetik Göttingen auf subtelomere Imbalancen untersuchten Patientenkollektivs. Teile der Arbeit wurden bereits von Auber et al. 2009 mit dem Titel "Identification of subtelomeric genomic imbalances and breakpoint …
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Real-Time PCR Combined with DNA Barcoding for the Authentication of Red Snapper (Lutjanus campechanus) Fillets
… substitution; however, it is expensive and time-consuming. A rapid, real-time PCR assay targeting red snapper was developed previously for use in fisheries management; however, it has not been tested for its ability to detect red snapper species substitution. The objective of this study was …
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Real Time PCR-Based Infectivity Assay and Characterization of Cell Surface Receptors for Turkey Hemorrhagic Enteritis Virus
… suffer from low sensitivity, inaccuracy, and time consumption. A SYBR Green-based real time PCR assay for determining the genomic titer of THEV through the quantification of its hexon gene was developed. The assay was applied as a quality control for the titration of splenic vaccines and was …
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Towards automatic gene synthesis with bioinformatics software, novel one-step real-time PCR assembly, and lab-chip gene synthesis
… melting temperature for both LCR and gapless PCR assembly of very long gene sequences. The potential mis-hybridization, hetero-dimer, homo-dimer and hairpin formations among oligonucleotides are screened by pair-wise sequence alignment. The utility of TmPrime is demonstrated by synthesizing …
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Evaluation of a Multiplex Real-Time PCR Assay to Simultaneously Detect Streptococcus pneumoniae and Staphylococcus aureus in Nasal Samples
… adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of <em>S. pneumoniae</em> and <em>S. aureus</em> from the same nasal sample is described.</p>
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Detection and Identification of Gastrointestinal Pathogens in Clinical Samples: Comparison Between two Laboratory Developed Multiplex Real-Time PCR Platforms
… and serological testing, are labor intensive, time-consuming, and lack specificity and sensitivity. In recent years, multiplex molecular testing has made its way into the clinical laboratory for detection and identification of enteric pathogens directly from clinical stool samples. Multiplex …
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The development, implementation and evaluation of a real-time PCR-based diagnostic service for viral causes of infectious intestinal disease
… issue resulting in significant morbidity and sometimes mortality each year. The economic costs associated are substantial. Laboratory diagnosis of viral IID is important as the many infectious and non-infectious causes cannot be reliably differentiated using clinical or epidemiological …
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MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS
… facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>
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Proportional assessment of X and Y chromosome-bearing spermatozoa in bull and boar ejaculates using conventional and real-time PCR techniques
… two collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis and image analysis was employed to determine %Y-CBS in ejaculates from two bulls collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology was used to quantify %Y-CBS in the same …
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Safety of Slow Release Lipopolysaccharide (LPS) Pellet in a Swine Model and Development of an Exogenous Reference Control for Real Time PCR
… to correct for sample-to-sample variation in real time PCR. Additionally, the ongoing development of suitable analytical methods is of great importance and a critical need, which would further assist in the development of an appropriate translational animal model.</p>
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Characterization of prokaryotic diversity in a chlorinated solvent-contaminated bedrock aquifer using denaturing gradient gel electrophoresis, ribosomal DNA sequencing, and real time PCR
… for changes detected in the groundwater was time, not depth. PMSF fingerprint analysis revealed depth-dependent clustering trend. DGGE profiles for Desulfuromonas sp. revealed an unexpected level of diversity. Sequence analysis of about 400 DGGE bands indicated the predominance of a …
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Real time PCR and fluorescent in situ hybridization in the detection of the physical tsate of human papillomavirus 16 and 18 in paraffin embedded cervical tissue
… therefore sought to determine the feasibility of real time-PCR (RT-PCR) as a molecular tool in detecting the physical state, episomal versus integration of HPV 16 and 18 DNA in cervical cancers. Tyramide amplified fluorescent DNA in situ hybridization (FISH) was used to look for evidence of HPV …
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