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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"rbcL"”.
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Assessment of microalgal ACCase and rbcl gene expression as a function of nutrient and metal stress
Submitted in fulfillment of the requirements for the degree of Doctor of Philosophy: Biotechnology, Durban University of Technology, Durban, South Africa, 2018.
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Testing the utility of the consortium for the barcoding of life's two 'agreed upon' plant DNA barcodes, matK and rbcL
… chloroplast gene regions (cpDNA), matK and rbcL, would serve as the DNA barcode for all land plants. This recommendation was accompanied by CBOL‘s call for continued testing of these two regions, along with other potential gene regions that may prove to be more effective barcode regions, …
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An in vitro transcription termination system to analyze spinach chloroplast promoters
… in vitro, the 3$\sp\prime$ end of the rbcL gene as well as various factor-independent transcription terminators from E. coli were fused to the spinach chloroplast rbcL promoter. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, …
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Development of Bioinformatic Tools and Procedures to Computationally Support DNA Barcoding
… pipeline, users will be able to align the rbcL and matK barcode regions with confidence, with only a minimal amount of manual editing required for matK. The results achieved for the rbcL were roughly the same for the standalone alignment tools tested, and for the matK gene the results were …
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Evaluating the Evolutionary and Genetic Relationships of the Andean Orchids of Northwestern Ecuador
… library contains 135 <em>matK</em> and 136 <em>rbcL</em> DNA Barcodes representing over 33 Orchidaceae genera. Sequence analysis shows percent species resolution was higher for <em>matK</em> (98.8%) than <em>rbc</em>L (70.24%), with a large portion of the unresolved species for the <em>rbcL</em> …
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DNA barcoding of Jatropha curcas
… ,5-bisphosphate carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and Arabidopsis thaliana. I found that the rbcL gene in J. cureas and the 3' end of L. …
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Impiego di tecniche innovative per la valorizzazione e caratterizzazione molecolare di specie vegetali ornamentali
… L analisi del DNA barcoding dei core markers, rbcL e matK, è stato adottato come primo passo per l identificazione di 100 taxa appartenenti a 20 famiglie. Un terzo marcatore, la regione intergenica trnH-psbA, è stato anche testato su 74 taxa, dove i core markers indicati hanno presentato …
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A molecular and morphological investigation of the red seaweed genus Porphyra (Bangiales, Rhodophyta) in the northwest Atlantic
… carboxylase-oxygenase large subunit (rbcL) gene and rbcL- rbcS spacer, the nuclear ribosomal small subunit (SSU), and internal transcribed spacer 1 (ITS1) regions were compared with other Porphyra taxa sequences available on GenBank. Sequences from four taxa were found to match Pacific …
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The embryology of Prionium serratum (L.f.) Drege ex E.Mey. (Juncaceae:Cyperales) and its implications for the phylogeny of Cyperales
… of Cyperales using embryology, morphology and rbcL sequence data. The embryology of Prionium conforms to that found in Juncaceae. The tapetum is glandular-secretory and microsporogenesis is simultaneous. Pollen is united in tetrahedral and cross tetrads and is tri-nucleate at release. The ovary …
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Identification and regulation of genes involved in carbon dioxide fixation in Rhodobacter sphaeroides
… ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL,S).
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Molecular systematics of the genus Hypnea (Rhodophyta) in South Africa, with the description of a new genus, Tenebris (Cystocloniaceae, Rhodophyta)
… samples and mitochondrial (cox1) and plastid (rbcL) DNA sequences were obtained. Sequences were assembled, aligned by eye, and analysed using maximum likelihood, Bayesian analyses, and genetic distance (GD) matrices for molecular analysis. Specimens were sectioned and photographed for …
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Tracking Floral Visitation Using DNA Barcodes
… loads using the plastid DNA barcode region rbcL. The identities of monospecific pollen loads were determined by comparing sequences to a local plant reference library containing 22 native and 27 non- native plant species. The overall recovery of rbcL sequences from pollen loads was …
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DNA Barcoding of Ayurvedic Herbal Supplements used for the Purported Treatment of COVID-19
… were tested with four barcoding regions (i.e., rbcL, matK, ITS2, and mini-ITS2) using two composite samples per product. The use of the combined loci resulted in the amplification of 85.2% of the products and identification of at least one species in 64.8% of the products (based on the combined …
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Characterization of Ulva (Ulvaceae, Chlorophyta) species cultured in commercial abalone farms in South Africa, and comparison with closely related wild species, using morpho-anatomical and molecular methods
… Ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL), Internal Transcribed Spacer of nuclear ribosomal DNA (ITS nrDNA) and the elongation factor tufA. The 12 cultured Ulva specimens belonged in the U. lacinulata clade with weak support value of 0.57 for PP in the rbcL tree, high support value of …
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Molecular phylogeny and morphological reconstructions of Plagiochilaceae (Jungermanniopsida) with hypotheses on biogeography and divergence times
… Marker von zwei verschiedenen Genomen (rps4 und rbcL aus dem Chloroplasten und ITS aus dem Kern) von 120 Plagiochila-Arten und 8 Arten anderer Gattungen der Plagiochilaceae sequenziert. Komplexe phylogenetische Analysen basierend auf bis zu sieben verschiedenen Evolutionsmodellen führen zu einer …
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Taxonomic and Phylogenetic Diversity of Pitcher Plant Bogs in Georgia's Coastal Plain
… 179 species), of which 254 were sequenced for rbcL and matK gene regions of the chloroplast genome to generate a phylogeny. Sequencing returned 197 successful sequences of both rbcL and matK gene regions, totaling to 207 sequences used to construct a phylogeny after including downloaded GenBank …
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A molecular phylogeny of the subfamily Arundinoideae (Poaceae)
… purpose, sequence data of the highly conserved rbcL gene was obtained from 22 taxa selected from the lineages identified by the rpoC2 study. Phylogenetic analysis of a total of 36 sequences resolved some of the relationships of the major clades, but other relationships were poorly supported. In …
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Phylogeny reconstruction of the Schoeneae (Cyperaceae) with a focus on southern African genera
… study both plastid and nuclear DNA sequences (rbcL, trnL-trnF, rps16, ITS and ETS) were analysed. New sequences were added to the matrix from Verboom (2006). Parsimony method was used for phylogeny reconstruction. Morphological characters were then optimised on the parsimony tree using both …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… markers: ribulose-1,5bisphosphate carboxylase (rbcL) and internal transcribed spacer 2 (ITS2), amplified with universal primer pairs in a two-step polymerase chain reaction (PCR) library preparation for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… markers: ribulose-1,5- bisphosphate carboxylase (rbcL) and internal transcribed spacer 2 (ITS2), amplified with universal primer pairs in a two-step polymerase chain reaction (PCR) library preparation for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated …
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