Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"rational protein design"”.
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A directed evolution design of target specificity and kinetic analysis of conformational transitions in the HhaI methyltransferase /
… mechanism have been addressed. First, using rational protein design and directed evolution approaches the specificity of the HhaI MTase, GCGC, has been changed to GCG by functional elimination of one of the two target recognition elements. In addition, the introduced structural changes …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… on TEs, we proposed two models to provide a rational framework for understanding TE-mediated macrocyclization. The research presented here describes the characterization of two non-ribosomal peptide synthetase (NRPS) TE domains and their use as biocatalysts for the chemoenzymatic synthesis of …
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High relaxivity biomolecule based contrast agents engineered for molecular functional magnetic resonance imaging
… two approaches for developing high relaxivity protein-based contrast agents to address the issues of low detectability. First, we coupled monoamine sensing with the disaggregation of superparamagnetic iron oxide nanoparticles (SPIOs). Ligand detection was imparted by integration of a monoamine …
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HCV-Diagnostik mittels genotypspezifischer Antikörper
… between HCV genotypes 1b, 2 and 3. The envelope protein E2 sequence of genotype 1b was isolated from patients blood whereas consenus sequences had to be deduced from all available genotype-2 and -3 sequences. Sequences were derived from all sequences available in the Los Alamos database. After …