Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"radiolabel"”.
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Catabolic substrate utilization patterns: varying humidity and dietary regimens in the American cockroach Periplaneta americana (L.) (Dictyoptera: Blattidae)
… consumption and body weight. Also, a series of radiolabel experiments, utilizing injected ¹⁴C-glucose and ¹⁴C-palmitic acid, were conducted to determine radiolabel content (after exposure to varying humidity and dietary regimens) in whole body, feces, ¹⁴CO₂, hemolymph, fat body tissue and …
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Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate)
… by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser …
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Toxicokinetics and structure/toxicity relationships of low molecular weight peptide toxins from cyanobacteria
… Two dose groups (75 and 25 $\mu$g/kg) were given radiolabeled plus nonlabeled 2H-MCLR IV and one dose group (75$\mu$g/kg) was given radiolabeled plus nonlabeled 2H-MCLR via an isolated ileal loop. At either IV dose, more than half the radiolabel from ($\sp3$H) 2H-MCLR present in the blood at one …
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Design, Synthesis and Biological Properties of Fluorinated Estrogens, Progestins and Androgens, Potential Imaging Agents for Receptor-Positive Mammary and Prostate Tumors (fluorine-18)
… in the development of a potential fluorine-18 radiolabel useful as a means of quantifying estrogen receptor in receptor-positive mammary tumors. The affinities of the compounds for estrogen receptor were measured using competitive radiometric binding assays and 17(alpha)-fluoromethyl estradiol …
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Extended mechanical stimulations of cartilage for growth and repair
… and uses L-³H-proline and ³⁵S-sulfate radiolabel incorporation rates to measure rates of biosynthesis. The results also demonstrate the feasibility of replacing the fetal bovine serum and L-ascorbic acid components of the typical explant feeding medium with ITS …
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Docetaxel uptake and modulation of P-gpmediated docetaxel efflux by tyrosine kinase inhibitors in human lung carcinoma cell lines
… relevant effects. A more sensitive radiolabel-based technique was then developed to examine lower, pharmacologically achievable concentrations (100-500 nM) of docetaxel. The radiolabel-based assay was then applied to examining docetaxel uptake in the DLKP and A549 NSCLC cell lines …
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Selective functionalization of azines via phosphonium salts
… on drugs, and tritium is installed as a radiolabel for metabolic studies in the pharmaceutical industry. The protocol of the reaction is simple, and it functions on a wide range of building blocks and complex molecules. Additionally, the tritiation protocol was effectively applied on a …
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Tomato Phytochemicals and Prostate Cancer Risk
… 14C-sucrose were developed to biosynthesize and radiolabel these carotenoids in tomato cell suspension cultures. DU 145 prostate tumor cells absorbed 14C-15- cis phytoene and an oxidized phytoene product and partially metabolized these compounds in vitro. To elucidate the biological roles of …
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The Membrane Polypeptides of the Vacuolar System: Composition and Recycling
… were separated on sucrose gradients, and their radiolabel was confined almost exclusively to the membrane rather than soluble contents. SDS-polyacrylamide gel electrophoretic analysis of the peptides iodinated from within PL demonstrated at least 24 species with molecular weights ranging from …
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Isotopic labeling of isoflavones from plant cell and organ culture for metabolic tracking in animal models
… In the present studies, we utilized 14C-radiolabeled crude isoflavone extracts to track the absorption, distribution, and elimination of kudzu and red clover isoflavones in rats. A dose of approx. 0.148 MBq (4µCi) of radiolabeled extract was found to be sufficient to be metabolically …
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Freshwater Invertebrate Assimilation Nutrients from Detrital Biofilms Under Light and Phosphorus Manipulation
… was to employ C and phosphorus (P) isotopic radiolabels to understand how microbial biofilm constituents (i.e heterotroph and autotroph) contribute C and P to detritivore nutrition under raised light and nutrient regimes. I conducted a feeding study using detritivorous caddisflies (i.e. …
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Spontaneous Phosphatidylcholine Exchange Between Vesicles and Micellar Lipid-Apolipoprotein Complexes
… Lipid exchange was followed by incubating radiolabeled vesicles (('3)H-PC, plus ('14)C-cholesterol oleate as a nonexchangeable marker) with unlabeled lipid-apolipoprotein complexes at a constant temperature of 37(DEGREES) C. Incubation mixtures were fractionated on Bio-Gel A-5 m columns at …
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Uptake and distribution of 3H-cholesteryl oleyl ether in tissues of cholesterol-fed New Zealand White and Watanabe Heritable Hyperlipidemic rabbits
… independent mechanisms, the influx of radiolabel was greatest in the adrenal. The aortas of both CH-FED and WHHL were active in 3H-COE uptake. In autoradiographs (ARGs), the cells closest to the lumen at the time of injection were most active in plasma CE uptake. Mechanisms for the …
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Synthesis of Novel Glycolipid Agonists of the Protein CD1d
… ThrCer with a biotin residue and with a 14C radiolabel, and our collaborators have used these derivatives to show that ThrCer behaves similarly to \(\alpha\)GalCer in endogenous lipid trafficking and its tissue distribution in vivo. We have also made advances towards the stereoselective …
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I. Molecular Probes for the Progesterone Receptor. II. Design and Synthesis of a Rigidly Constrained Peptidomimetic as a Potential Beta-Turn Mimic. III. Isolation and Characterization of Novel Ion Channel Modulators From Commercial Phenol Red Preparations
… progestin ORG 2058. Metabolic stability of the radiolabel was indicated by the low radioactivity levels seen in bone. The structures of two novel steroids (17$a\beta$-hydroxy-16$\alpha,17 \alpha$-oxacyclopentan-1$\sp\prime$-one-D-homoandrost-4-en-3-one (78) and …
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Substrate specificity and functional characterization of sodium/dicarboxylate cotransporters
… and used both electrophysiological and dual-radiolabel competitive uptake techniques to exam their transport properties. My work indicates that different multiple transmembrane helices (TMs) are involved in NaDC1 substrate recognition, with the region of TM 3-4 and the C-terminus required for …
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Novel Interactions of the Hormone Leptin Revealed by PET Imaging in Rodents and Rhesus Macaques
… in mammals, we have developed methodologies to radiolabel the hormone and visualize its biodistribution using positron emission tomography (PET). Two complementary techniques were developed to label leptin using the positron emitting isotopes 68Ga and 18F. 68Ga labeling was accomplished by …
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Characterization of the Fast Axonally Transported Proteins in the Rat Optic Pathway
… understand fast transport trafficking, the FT of radiolabeled retinal ganglion cell (RGC) proteins in the adult rat optic pathway was studied. The optic pathway model system afforded the opportunity to delineate FT trafficking in the axon from FT trafficking at pre-synaptic terminals. Radiolabel …
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Novel Amino Acid Transporter-Targeted Radiotracers For Breast Cancer Imaging
… potentials in breast cancer imaging. In order to radiolabel tyrosine and AMT, the chelators N,N’-ethylene-di-L-cysteine (EC) and 1,4,8,11-tetra-azacyclotetradecane (N4 cyclam) were selected to coordinate <sup>99m</sup>Tc. These chelators have been reported to provide stable chelation ability with …
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Characterization of IphP from Nostoc commune UTEX 584 and a Dual Specificity Protein Phosphatase from Anabaena PCC 7120
… phosphoproteins of ~120 and 55 kDa that had been radiolabelled in vitro were dephosphorylated by partially purified PAD. PAD activity varied in vivo ~5-fold in a rhthymic, seemingly diurnal manner. Periplasmic proteins, including the 55kDa protein, were labelled in vivo and the degree of …
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