Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"radical SAM"”.
-
Sensitivity of Radical SAM Enzyme MftC to Molecular Oxygen
<p>MftC is a radical S-adenosyl L-methionine (SAM) enzyme that catalyzes the first step in the production of mycofactocin. It is a ribosomally synthesized post-translationally modified (RiPP) redox cofactor shown to be essential for the survival of bacteria in the <em>Mycobacterium</em> genus in …
-
Biochemical and structural characterization of radical SAM enzymes Elp3 and viperin
Made available in DSpace on 2017-03-01T17:00:49Z (GMT). No. of bitstreams: 2 SELVADURAI-DISSERTATION-2016.pdf: 4094456 bytes, checksum: 0f8ac9e151d2ca2bcc0b86f28ca3e24e (MD5) LICENSE.txt: 4217 bytes, checksum: 97822b5b33afa06a1dd64bba5cddd611 (MD5) Previous issue date: 2016-08-19
-
Biosynthetic and bioinformatic investigation of RiPPs with radical SAM-installed crosslinks
… to the emerging antimicrobial resistance crisis. Radical S-adenosylmethionine enzymes (rSAM) are responsible for producing intriguing and complex molecules, some of which are antibiotics. These enzymes have been demonstrated to catalyze C-C, C-S, C-O-C, and C-N crosslink formation among other …
-
Investigation of radical SAM-mediated thioether installation in RiPP natural products
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-05-01
-
Biochemical and structural characterization of radical SAM enzymes Elp3 and viperin
The radical SAM (S-adenosylmethionine) superfamily consists of a diverse set of enzymes which use [4Fe-4S] and SAM to initiate radical transformations. These diverse enzymes undergo a common series of steps where SAM is cleaved to yield methionine and a 5’-deoxyadenosyl radical intermediate …
-
A Bioinformatics Approach to Identifying Radical SAM (S-Adenosyl-L-Methionine) Enzymes
Radical SAM enzymes are ancient, essential enzymes. They perform radical chemical reactions in virtually all living organisms and are involved in producing antibiotics, generating greenhouse gases, human health, and likely many other essential roles that have yet to be established. A wide variety …
-
Post-translational generation of Cá-formylglycine in Prokaryotic Sulfatsases by Radical SAM-Proteins
… Motiv. AtsB, ein vorausgesagtes radikales SAM Eisen-Schwefel Protein, ist ein cytosolic Protein und wird ausschließlich für Änderung von Serin 72 im arylsulfatase AtsA von K. pneumoniae angefordert.AtsB wirkt physikalisch auf AtsA in einer Weise des Serins 72-dependent ein, wie in gezeigt …
-
Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms
-
Investigating the chemistry of lipoyl synthase
The radical SAM protein lipoyl synthase (LipA) is essential for lipoic acid biosynthesis via sulfur insertions into the unactivated C6 and C8 centres of a protein-bound octanoyl group. Using an in vitro assay which makes use of a small peptide mimic of the protein substrate, it has now been shown …
-
Chemical Modification of Iron-Sulfur Cofactors
… a facile method for incorporating ⁵⁷Fe into the SAM-binding cluster of a radical SAM methyltransferase, RlmN. In Chapter 3, we show that two [Fe₄S₄] clusters of BtrN, a Twitch-domain-containing radical SAM enzyme, have different rates of Fe exchange, and by utilizing such difference, we …
-
Organometallic Chemistry in Fe–S Clusters
… in several classes of enzymes, including radical S-adenosyl-L-methionine (SAM) enzymes and enzymes involved in terpene biosynthesis. Due to the transient nature of these intermediates, full characterization of the geometric and electronic structures of enzymatic [Fe₄S₄]–alkyl species has …
-
Studies of unusual catalysis : a tale of four enzymes from diverse biosynthesis pathways
… Fom3 and OxsB, belong to the cobalamin-dependent radical SAM class of enzymes. While these enzymes are believed to operate with unprecedented chemistry, they remain poorly understood and understudied. Fom3 is proposed to methylate an unactivated carbon center in the biosynthesis of fosfomycin, …
-
Untersuchungen zur Biosynthese und Regulation von Oligosaccharid-Antibiotika
… Die Inaktivierung führte jeweils zum Zusammenbruch der Avilamycin-Biosynthese. Bei der Überexpression von aviC1 im Wildtyp wurde die Avilamycin-Biosynthese um das 4,6-fache gesteigert, bei gleichzeitiger Überexpression beider Regulatoren stieg die Avilamycin-Biosynthese um das 4,8-fache. …
-
Investigating the Distribution and Biosynthesis of Modified F<sub>430</sub> Cofactors in Methanogenic and Methanotrophic Archaea
Methanogenesis is the biological production of methane and is utilized by methanogenic archaea (methanogens) to generate energy. This process is responsible for 70% of total atmospheric methane, a potent greenhouse gas and an important energy source (natural gas). In the future, reversing …
-
Exploring Prokaryotic Thiamin Biosynthesis: Mechanistic Studies On Thiamin Thiazole Synthase And Pyrimidine Synthase
… cluster containing enzyme, and belonged to the radical SAM family. The 4Fe-4S cluster binding motif (CX2-CX4-C) of ThiC is different from the motif (CX3-CX2-C) conventionally used by the other established members of this family. With the pure protein with a well-reconstituted Fe-S cluster, we …
-
Prebiotic photoreduction and polymerization of cysteinyl peptides.
… examples of such an arrangement are the radical SAM (S-adenosylmethionine) enzyme and aconitase being an enzyme of the citric acid cycle. We designed and synthesized peptide sequences that could coordinate a [4Fe-4S]2+ cluster with three cysteinyl ligands, leaving an open coordination …
-
Post-translational modifications of methyl-coenzyme m reductase in methanosarcina acetivorans
… was shown to be synthesized by a B12-dependent radical SAM (rSAM) enzyme, designated MgmA. In Chapter 2, we show that two different MgmA homologs are able to methylate MCR in vivo when expressed in Methanosarcina acetivorans, an organism that does not normally possess this PTM. M. acetivorans …
-
Studies on lipoic acid metabolism
… atoms at carbons 6 and 8 with sulfur atoms using radical SAM chemistry. Alternatively, either exogenous lipoic acid or octanoate can be directly attached to the LDs by lipoate-protein ligase (LplA) via an acyl–AMP intermediate. E. coli strains containing null mutations in lipB are auxotrophic for …
-
Investigations of metalloenzymes involved in bacterial natural product biosynthesis
… redox-active metal ions to initiate and control radical chemistry, breaking inert C-H bonds and rearranging carbon skeletons with exquisite specificity. This work describes the characterization of two such reactions in the biosynthesis of two different natural products: fosfomycin, a clinically …