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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 273 for “"rDNA"”.
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Computational Discovery and Analysis of rDNA Sequence Heterogeneity in Yeast
Ribosomal RNA genes, known as ribosomal DNA or rDNA, are commonly found in tandem arrays of hundreds of repeating units. The sequences of each unit in an array were thought to be near-identical but it is now known that frequent mutations may occur, causing heterogeneity amongst units. Opposing …
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The Characterization of Circular Extrachromosomal rDNA Elements in Naegleria SPP
… characterized by a circular extrachromosomal rDNA element (CERE) that is localized to the nucleolus. The CERE is unique because it contains the genes for rDNA production, which are not found in the chromosome. Naegleria species contain thousands of copies of the CERE which can replicate …
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Sequence and Functional Structures of the Extrachromosomal rDNA Minichromosome of Physrum Polycephalum
… palindromic extrachromosomal DNA molecules (rDNA), which have been isolated both as a satellite DNA molecule, and as a "minichromosome" with associated chromatin proteins. The genes coding for the 19 S, 26 S, and 5.8 S rRNAs are transcribed as a single, large precursor molecule, beginning …
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… tissue by DNA sequencing of the initial 16S rDNA amplification products. One way to solve this problem is to use denaturing gradient gel electrophoresis (DGGE) to separate the PCR products before DNA sequencing. Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on …
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A molecular phylogeny of the families and orders of Collembola (Arthropoda: Hexapoda) using the 18S rDNA gene
In this study I investigate the phylogenetic relationships of the families and orders of Collembola, with special reference to six groups that traditionally have been problematic, or that have become problematic with the implementation of the cladistic philosophy to the systematics of the group. …
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The Isolation and Characterization of the Microbial Flora in the Alimentary Canal of <em>Gromphadorhina portentosa</em> Based on rDNA Sequences.
<p>Multicellular organisms are not single individuals but carry a complex natural microflora with them. This complex's diversity and function can be considered a distinct ecosystem. Traditional methods of isolation and identification miss >90% of the actual diversity. This study uses the gut …
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Identifying individual ribosomal RNA gene repeats to investigate their behaviour
… In eukaryotes, the ribosomal RNA genes (rDNA) are present as multiple tandem repeats that are transcribed by a dedicated polymerase, RNA polymerase I. While hundreds of rDNA repeats are present, only a subset is transcribed in a cell at any given time, with the transcriptional status …
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Phenotypic and transcriptomic consequences of ribosomal DNA copy number variation in Caenorhabditis elegans and Saccharomyces cerevisiae
Ribosomal DNA (rDNA) forms the template for mature ribosomal RNA (rRNA) and shows considerable sequence conservation through evolution. In eukaryotic genomes rDNA is present in one or more tandem repeat arrays, with copy number variation (CNV) commonly observed in inter- and intra-species …
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The recombinant DNA case: balancing scientific and political decision-making
… research technique, recombinant DNA (abbreviated rDNA) has opened up new vistas in biological and other fields of research. But its potential yet unproven hazard has created uneasy feelings toward the technique. The controversial nature of the issue finally launched rDNA into the political sphere, …
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Effects of retinoic acid on ribosomal RNA gene expression during limb regeneration
… (N), and 3-finger bud (3-FB). Xenopus nuclear rDNA and axolotl 16S mitochondrial rDNA fragments were used to detect rDNA and rRNA during regeneration. Nuclear rDNA was amplified during the MB stage of regeneration in the absence of RA. The amplified rDNA did not appear to be contributing to the …
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Ribosome Heterogeneity in Zebrafish Germ and Soma provides insight into Gene Expression during Development and Disease
… expression of two zebrafish ribosomal DNA (rDNA) genes was identified (Locati et al. 2017b). One rDNA variant located on chromosome 4 was found to be specifically expressed in eggs (aka maternal type), while expression of another rDNA variant located on chromosome 5 was specific to somatic …
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Comparative genomics of repetitive elements between maize inbred lines B73 and Mo17
… including centromere, 45S ribosomal DNA (rDNA), knob, and telomere repeats. Genotype specific 45S rDNA sequences were discovered. The B73 and Mo17 polymorphic k-mers were used to examine allele-specific expression of 45S rDNA in the hybrids. Although Mo17 contains higher copy number than …
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Caracterização e análise filogenética molecular de novos isolados fixadores de nitrogênio
… do gene para o RN A ribossomal de 16S (16S rDNA) e região intergênica dos genes para os RNAs ribossomais de 16S e 23S (região intergênica 16S-23S rDNA) foi realizada no presente trabalho a partir das técnicas de Polimorfismo de Fragmentos de Comprimento Aleatório amplificados (PCR-RFLP), …
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Molekulare Identifizierung von Neisseriaceae und Moraxellaceae mittels ribosomaler DNA-Sequenzierung
… partieller Abschnitte der 16S- oder 23S-rDNA können Bakterien meist exakt spezifiziert werden. Hauptziel der vorliegenden Arbeit war es, hypervariable rDNA Abschnitte zu finden, die von stark konservierten Regionen flankiert werden, um auf molekularer Ebene Mitglieder der Familie …
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Comparative Serological and Molecular Analysis of Group VIII <em>Spiroplasma</em> from Australia and North America
… by serology. Recent work has generated 16S rDNA sequences that generally correlate with the serological findings. Although the serology and the 16S rDNA sequence analysis clearly classifies strains to the group level, they do not distinguish between strains within the same group. The goal of …
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Silencing and recombination in yeast ribosomal DNA
… system for the maintenance and homogenization of rDNA. Eliminating mutations and heterogeneity in rDNA repeats is necessary evolutionarily, but harmful to individual organisms--and has been linked to aging-related pathology. We present a forensic investigation of this process using a new …
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Molecular phylogenetics of the Metazoan clade Lophotrochozoa
… nuclear ribosomal large-subunit RNA gene (LSD rDNA). Results presented provide the first molecular evidence for a close relationship between the Scaphopoda and Cephalopoda. Phylogenetic trees with this topology were found to have likelihood scores significantly better than those for phylogenies …
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DNA repair and genomic instability in yeast aging
… to be evolutionarily conserved. Extrachromosomal rDNA circles (ERCs) have been identified to be a cause of replicative aging in budding yeast S. cerevisiae. Genomic instability can cause ERCs to excise from chromosomal rDNA arrays, containing 35S and 5S rRNA genes. At each cell division, ERCs …
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Molekularsystematische Untersuchungen an Vertretern der pflanzenparasitischen Gattung Exobasidium (Basidiomycota)
… wurden Sequenzvergleiche der Domäne I der 26S rDNA, der 18S rDNA und der ITS-Region durchgeführt. Übereinstimmung in den PCR-RFLP-Mustern des 5’-Bereichs der 26S rDNA zeigt Konspezifität an, da solche Stämme einen ähnlichen GC-Gehalt und hohe DNA-DNA-Homologien aufweisen. Insgesamt wurden über …
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