Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"quiescent cells"”.
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A Numerical Study of a Delay Differential Equation Model for Breast Cancer
… immune response to the growth of breast cancer cells and investigate the impact of certain drug therapies on the cancer. We use delay differential equations to model the interaction of breast cancer cells with the immune system. The new model is constructed by combining two previous models. The …
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NON-GENETIC MECHANISMS OF CHEMORESISTANCE IN ACUTE MYELOID LEUKEMIA
… leukemic drugs. Drug resistance in leukemic cells has been associated with intratumoral heterogeneity, among which quiescence, specifically, is considered a key factor for cell survival. Experimental evidence collected both from patients and model systems suggests that relapse is due to rare …
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Valutazione dell'efficienza, efficacia e sicurezza di vettori lentivirali nel trasferimento del gene CFTR in sistemi modello di epitelio respiratorio in fibrosi cistica
… of therapeutic gene and they are able to infect quiescent cells like respiratory epithelium cells. Primary goal of the project is concerned with the realization of a lentivirus working live agent, transferring CFTR WT gene, or SHRNA molecules directed against ENAC subunits; ENAC is a sodium …
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Enhancement of death receptor-mediated apoptosis in multiple myeloma cells
… using Hoechst 33342/PI staining. TRAIL-resistant cells were generated by acute exposure of TRAIL sensitive cells to TRAIL followed by the selection of TRAIL-resistant cells (TRAILR). Apoptotic effects in quiescent cells (labelled as PKH26Hi) were also determined. Subsequently, an investigation was …
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Crosstalk between E2F3 and p19ARF/p53 in the regulation of cell cycle progression and tumorigenesis
… through the generation of compound mutant cells and mice. The nature of the relationship between E2F3, p19ARF, and p53 was highly context dependent. E2F3 is required to repress pl9Arf expression in quiescent cells. This places E2F3 upstream of p19ARF and p53 during cell cycle re-entry.
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The Src- and Abl- Dependent Regulation of Net1A In Breast Cancer
… of which are which sequestered in the nucleus in quiescent cells to prevent aberrant RhoA activation. Many cell motility stimuli drive cytosolic relocalization of Net1A, but mechanisms controlling this event are not fully understood. Here we demonstrate that EGF stimulates Src- and Abl1-dependent …
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Uncovering the Importance of Cell Morphology, Genetic Regulation, and Neuronal Signalling in Quiescent Neural Stem Cells
Neural stem cells (NSCs) are essential for their ability to produce new neurons during neurogenesis. These cells undergo quiescence, the state when quiescent cells are metabolically active but do not produce new neurons. Understanding and manipulating this critical state is essential due to …
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Serum Regulation of Inhibitor of DNA Binding/Differentiation 1 Expression by a BMP Pathway and BMP Responsive El
… Genes (IEGs) are expressed upon re-entry of quiescent cells into the cell cycle following serum stimulation. These genes are involved in growth control and differentiation and hence their expression is tightly controlled. Many IEGs are regulated through Serum Response Elements (SREs) in their …
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Developmental Appearance of Phosphorylated Intermediate Filaments in Nuclei of Glioma and Neuroblastoma Cells
… skeleton in the nucleus of eukaryotic cells, termed the nucleoskeleton, is only vaguely known. Investigations into the role of intermediate filaments (IF) in the nucleus have primarily focused upon lamins, however some studies have suggested that there may be a role for non-lamin …
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Non-Histone Nuclear Proteins : Patterns of Synthesis and Phosphorylation During the Cell Cycle, Redistribution at Times of Gene Activation and Properties of Subsets Interacting with HnRNA
… according to cell type. Actively proliferating cells with high transcriptional activity contain considerably more nonhistone nuclear proteins than do quiescent cells. In every cell type, the nuclear proteins are heterogeneous ; recent estimates based on two-dimensional gel electrophoresis place …
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Cell Cycle Progression Effects of Albumin
… through late G<sub>1</sub> into S-phase. Quiescent BJ-hTERT human fibroblasts primed back into G1 with FBS treatment, were treated with a mixture of lipids and carrier bovine serum albumin (BSA) along with [3H]-thymidine deoxyribose ([3H]-TdR) to monitor progression into S-phase. …
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Transcriptomics-based study reveals a novel link between arginine methylation and phosphate regulation in Saccharomyces cerevisiae
… profiles of wild type versus Hmt1-null cells. In mid-log exponential growth, we found dysregulation of phosphate metabolism and polyphosphate accumulation in the Hmt1-null strain. Acid phosphatases (PH05, PH011 and PH012), phosphate transporters (PH084 and PH089) and the vacuolar …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
Eukaryotic cells have evolved a complex set of regulatory proteins to maintain genome stability by regulating DNA replication once and once per cell cycle. Despite intensive investigation, a precise understanding of the proteins that are phosphorylated during the initiation phase of DNA replication …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… is an essential mechanism that helps protect cells from a variety of environmental stresses. Cell death can result if cells are not able to properly adapt and protect themselves against adverse stress conditions. Failure to properly deal with stress has implications in human diseases including …
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Studies on mammalian pre-mRNA splicing : connections to transcription and cancer
… pertaining to pre-mRNA splicing in mammalian cells. The first part examines the regulation of the alternative splicing of the PKM gene in cancer cells, while the second part investigates the physical connections between the transcriptional apparatus and splicing factors. Cancer cells uniformly …
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Characterization of Fasting-Induced p21 Expression and Protection of Intestinal Stem Cells
… of <italic>p21</italic>, imaging of reporter cells demonstrated that p53 prevented the ERK/MAPK pathway from activating <italic>p21</italic> expression when quiescent cells were stimulated with serum to re-enter the cell cycle. In addition, low light bioluminescence imaging identified …
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Modelling chromatin dynamics in neural stem cell quiescence and reactivation
Majority of adult mammalian neural stem cells are quiescent, remaining in an actively maintained state of cell cycle arrest. Quiescent and actively proliferating neural stem cells can be distinguished by their transcriptional state, however, the understanding of how these gene signatures are …
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Characterization of Thy-1 expression on human hematopoietic cells
Hematopoietic stem cells have extensive proliferative capacity and the ability to produce daughter cells of all hematopoietic lineages. However, their characterization is complicated by their low frequency and the heterogeneous cellular composition of the tissues in which they are found. The …
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Mutational processes in normal human tissues
Mutations accumulate in all cells throughout life from the very first cell division of the fertilised egg. These somatic mutations contribute to cancer and other diseases as well as provide insights into ageing and development. Although somatic mutations in cancer genomes have been extensively …
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Repression of E2F-4 Mediated Transcription and Growth by Hypophosphorylated pRb2/p130 in Human Prostate Cancer Cells
A fundamental property of tumor cells is the ability to proliferate in an unscheduled manner, circumventing the cues which would otherwise prevent entry into, and completion of, the cell cycle (Malumbres, Barbacid 2001). The retinoblastoma protein (pRb), and its homologues pRb2/p130 and p107, …
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