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Showing 1 to 20 of 31 for “"quantitative real time polymerase chain reaction ("”.

  1. Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice

    정, 종갑2016

    … 3. Immunoblot analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs 13 6. Uptake of 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino-2-deoxyglucose (2-NBDG) 14 7. Animal experiments 14 8. FGF21 secretion 15 9. IL-1β assay 15 10. Statistical …

    ajou Repository record

  2. The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor

    James, Kris MThe University of Western Ontario2013

    … E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal …

    uwo Repository record

  3. Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells

    Fang, Siteng

    … the stem cell differentiation period tested. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated the scaffolds developed preferentially support iPSCs to differentiate into mesoderm. Taken together, the study has developed collagen-based scaffolds that support iPSC …

    binghamton Repository record

  4. Biomarkers of Human Exposure and Effect to Petrogenic Polycyclic Aromatic Hydrocarbons

    Yap, Dianne Aster YunqueThe University of Texas Medical Branch at Galveston2021

    … Next Generation Sequencing (NGS) and quantitative Real Time – Polymerase Chain Reaction (qRT-PCR) were used to identify and verify differentially expressed circulating plasma microRNAs that may serve as novel minimally invasive biomarkers of effect following exposures to bioactive PAHs …

    utmb Repository record

  5. Characterization of downstream target genes regulated by ABF-1 in different states of B cell development

    Eusebio, Anthony R.2005

    … RAJI, as well as the plasma cell line, ARH-77. Quantitative real-time polymerase chain reaction and DNA microarray technology was used to investigate target genes that are subjected to transcriptional regulation by ABF-1. Using ABF-1 inducible cell lines or B cell lines that overexpress ABF -1 …

    u-pacific Repository record

  6. Phakopsora pachyrhizi growth in tissue of leaves from resistant and susceptible soybean germplasm

    Lu, FanglinUniversity of Illinois at Urbana-Champaign2013

    … more sporulating uredinia indicate a susceptible reaction. Resistance to soybean rust is characterized by the development of reddish-brown (RB) lesions or a symptomless immune response, though considerable variation in lesion diameter, color and sporulation has been observed among resistant …

    uiuc Repository record

  7. Gene Expression in Endometrial Tissues of Normal Mares and Mares With Delayed Uterine Clearance

    Gray, Giles AnthonyVirginia Tech2006

    … the uterine lumen following breeding events, venereal disease or an estral cycle. This syndrome is typically seen in older, multiparous mares and mares with poor reproductive tract conformation. The etiopathogenesis of DUC has not been fully elucidated but suggested causes include poor genital …

    vt Repository record

  8. Genetic Variations in Interferon-Induced Genes and HCV Recurrence after Liver Transplantation

    Whitehill, Benjamin Cameron2014

    … and clinical complications post-transplantation. Quantitative real-time polymerase chain reaction (QPCR) was also run to determine the relationship between SNPs or disease state and the level of RNA expression. Results were collected and analyzed using Fishers exact test, Kaplan-Meir method, and …

    vcu Repository record

  9. Impact of environmental conditions on expression levels of resistance-nodulation-division (RND) efflux pumps and outer membrane porins in Acinetobacter baumannii

    Bazyleu, AndreiUniversity of Ontario Institute of Technology2012

    … with the MDR and biofilm-forming phenotypes. The quantitative-real time polymerase chain reaction analysis revealed that levels of iron(III) in growth medium, oxidative stress and ethanol shock were amongst the most potent factors capable of influencing expression of RND efflux pumps and outer …

    uoit Repository record

  10. Molecular genetics of abdominal aortic aneurysm

    Chinien, GanessenKing's College London2012

    … of these differentially expressed genes using quantitative real time polymerase chain reaction (qRT-PCR). Methods RNA samples were prepared from full thickness aortic walls obtained during open repair of AAA, aortic bypass for AOD and transplant patients for NA. The quality of the RNA was …

    kings Repository record

  11. Evaluation of Pectin Methylesterase and Lactobacillus spp. Isolated from Tabasco Peppers (Capsicum frutescens L.)

    Jones, Sonja TaneeSchool of Nutrition and Food Sciences2017

    … product. Failures in pectin stability can sometimes be related to the presence of enzymes. Understanding how the pectin enzymes function during fermentation of pepper sauce is a necessary step in controlling the enzymatic activity, and thereby reducing production cost and the risk of having a …

    lsu-thes Repository record

  12. Human Organotypic Retinal Cultures as a model of human retinal ganglion cell degeneration in glaucoma. Effects of epigenetic regulation and mesenchymal stem cell derived growth factors.

    Hopes, MarinaUniversity of East Anglia2014

    … cell gene expression was examined. Methods: Quantitative real-time polymerase chain reaction (QRT-PCR) was used for assessment of retinal cell marker genes expression, immunohistochemistry and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labelling (TUNEL) for …

    east-anglia Repository record

  13. Identification of Drought-Responsive Genes and Validation for Drought Resistance in Rice

    Batlang, UtlwangVirginia Tech2010

    … the maize reproductive tissue were validated by quantitative real time polymerase chain reaction (qRT-PCR). The differentially expressed genes common to both maize and rice were further analyzed by gene ontology analysis to reveal core biological processes involved in drought responses. In both …

    vt Repository record

  14. Differentiation and contractility of colon smooth muscle under normal and diabetic conditions

    Touw, Ketrija2013

    … increase resulted in decreased myosin light chain phosphorylation, thus explaining the decreased contractility of the colon. Chronic diabetes was also associated with increased basal calcium levels. Western blotting and quantitative real time polymerase chain reaction (qRT-PCR) analysis …

    iupui Repository record

  15. Characterization of the A/B regulon in tobacco (Nicotiana tabacum)

    Reed, Deborah G.Virginia Tech2003

    … regulatory mutant backgrounds that differ at two quantitative alkaloid loci, A and B. In order to identify novel genes that are differentially expressed during alkaloid biosynthesis, the transcriptional profiling procedure, fluorescent differential display (FDD), was used to screen total RNA …

    vt Repository record

  16. The Effect of Compound L19 on Human Colorectal Cells (DLD-1)

    Mohammadhosseinpour, Sepideh2019

    … the mechanism of apoptosis was determined using quantitative real-time polymerase chain reaction (qRT-PCR) and gene specific primers for caspases 6, 8, 10 (extrinsic pathways), 9 (intrinsic), 1, 4, 5 and 12 (ER stress). RT<sup>2</sup> Profiler PCR array for human apoptosis genes was used to …

    sfasu Repository record

  17. Quantitative Real-Time PCR Analysis of Colchicine Pathway and Rhizome Developmental Genes

    Rodriguez Diaz, DianaUniversity of Houston2021

    … and rhizome development, respectively. Using quantitative real-time polymerase chain reaction (qRT-PCR), a comparative analysis of gene expression in G. superba may reveal differential gene expression of selected candidate genes. Reference genes were selected and analyzed for normalization of …

    houston Repository record

  18. Effects of low linolenic soy oil on pre-malignant human mammary epithelial cell progression

    McCall, Elaine TeresaVirginia Tech2008

    … synthase (FAS) in tumors were analyzed using quantitative real time-polymerase chain reaction (qRT-PCR). We found that dietary LLSO supplementation significantly (p < 0.05, Tukey's test) increased tumor expression of oncogenes HER2/neu, EGFR, FAS, and H-ras, but not in the SO or lard …

    vt Repository record

  19. The Role of Base Modifications on Tyrosyl-tRNA Structure, Stability, and Function in Bacillus subtilis and Bacillus anthracis

    Denmon, AndriaRice University2013

    … antibiotic sensitivity tests, growth curves, and quantitative real-time polymerase chain reaction (qRT-PCR) were employed. Strains deficient in ms2 showed comparable growth to the parent strain when cultured in defined media, but Q deficient strains did not. The loss of ms2i6A37 conferred …

    rice Repository record

  20. Identification of growth-related tonoplast proteins in Arabidopsis thaliana

    Arvidsson, Samuel JanneUniversität Potsdam

    … for high-throughput reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) was developed by selecting specific primer pairs. To this end, a software tool (called QuantPrime, see http://www.quantprime.de) was developed that automatically designs such primers and …

    potsdam-diss Repository record

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