Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"quantitative proteomics"”.
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Management, visualisation & mining of quantitative proteomics data
… Having conquered a means of documenting a static proteomics snapshot of cells, the proteomics field is progressing towards understanding the extremely complex nature of cell dynamics. PepTracker facilitates this by providing the means to gather and analyse many protein properties to generate new …
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Benchmarking methods for label-free quantitative proteomics data analysis
… proteins. Mass spectrometry (MS)-based proteomics is the predominant technology for protein quantification. In particular, MS-based label-free quantification (LFQ) proteomics can quantify thousands of proteins in complex biological samples. However, the molecular heterogeneity of such …
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Hypothalamic Transcriptional Profiling and Quantitative Proteomics of Mice under 24-Hour Fasting
… using microarray technology. Furthermore, quantitative proteomics were used to identify differential protein levels during fasting, and in a genetically obese mouse model, Mice were given either a 24-hour fast, or ad libitum access to food. Hypothalamic RNA and microRNA samples were …
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Quantitative proteomics analysis in cultured skin fibroblasts from patients with rare genetic disorders
… are used as a cell model for the disease. A proteomics quantification method is applied to study the pathogenic alterations in these cells.
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Quantitative Proteomics Analysis of Differentially Expressed Proteins in Aβ(17-42) Treated Synaptosomes
… treated synaptosomes and control. By using proteomics, we have also identified several biomarkers including aldehyde dehydrogenase, aldolase, α-enolase, heat shock cognate 71, peptidyl-prolyl cis-trans isomerase, and ATP synthase α chain. Our present findings, suggest the role of Aβ(17-42) …
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A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
Objectives: 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins which may be responsible for the synergism seen between cytokine and injury in …
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Deciphering Metabolic Adaptations in Salmonella Enterica Serovar Typhimurium 14028S During Batch Growth and Gentamicin Exposure Using Absolute Quantitative Proteomics
Understanding the mechanisms underlying antibiotic-mediated bacterial cell death is crucial for the development of novel strategies to control bacterial infections. Although the primary targets of antibiotics are well known, the actual molecular events leading to bacterial growth inhibition and …
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Integration Of Quantitative And Functional Proteomics To Explore The Global Functional Landscape Of Post-Translational Modifications
Proteomics explores global-scale protein functions, with post-translational modifications (PTMs) expanding proteome complexity and functionality. Significant advancements in mass spectrometry (MS)-based quantitative proteomics have expanded the scope of PTM pathways. Meanwhile, functional …
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THE BIO-MOLECULAR ANALYSIS OF TITANIUM DIOXIDE NANOPARTICLES EXPOSURE ON CARDIOMYOCYTES
… to work. To approach this bottleneck, the use of quantitative proteomics approach using isobaric tags for relative and absolute quantitation (iTRAQ™) was proposed to identify these biological endpoints. Using the H9c2 cardiomyocyte as an <i>in vitro</i> model, titanium dioxide (TiO₂) NPs was …
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Alternatively spliced isoforms of Fibronectin, Tenascin-R and other potential players in early vasculogenesis
… embryos, enriched for ECM content, and used quantitative proteomics to analyze their ECM composition. From these data, we identified a set of matrisome proteins that had decreased abundance in EIIIA/B double-null yolk sacs but were relatively unchanged in single-null and heterozygous yolk …
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The development and application of mass-spectrometry-based tools to monitor proteome remodeling in microbes
… As such, there is a need for approaches that can quantitatively resolve the contributions of protein synthesis and protein degradation pathways in a wide array of cellular and environmental contexts. Quantitative proteomics via mass spectrometry stands out as a powerful tool for deciphering these …
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Twist of messenger Fate: novel mechanisms for TDP43 in modulating mRNA decay and alternative polyadenylation
… does it cause specific motor neuron death? Our quantitative proteomics analysis of the TDP43 interactome revealed the interaction with components of the mRNA surveillance pathway, suggesting a still undiscovered function in nonsense-mediated decay. We demonstrated that TDP43 acts translation- …
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Functional analysis of the histone variant H2A.Z during lineage commitment
… is crucial for the response to stimuli. Using quantitative proteomics, we uncovered a role for H2A.Z ubiquitylation in modulating the protein interaction landscape of H2A.Z. H2A.Zub impacts a specific subset of protein interactions. By investigating several of these differential interactions, …
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The role of ClpXP-mediated proteolysis in resculpting the proteome after DNA damage
… selection by ClpXP. These experiments combine quantitative proteomics and use of an inactive variant of ClpP to "trap" cellular ClpXP substrates and compare their relative levels with and without DNA damage. Analysis of trapped substrates reveals that cellular stress can result in dramatic …
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Elucidating the molecular mechanisms of p110δ activation in T cell antigen receptor signalling
… p110 isoforms from primary murine lymphocytes. A quantitative proteomics approach was subsequently used to identify endogenous proteins that interact with p110δ in primary activated CD4+ T cells upon TCR stimulation. This analysis has identified multiple adaptor proteins and co-stimulatory …
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Comprehensive Proteomic Analysis and Characterization of Human Bone Marrow Mesenchymal Stem/Stromal Derived Extracellular Vesicles
… the protein composition of hBM-MSC-EVs using a quantitative proteomics analysis. hBMMSC-EVs were produced from 5 hBM-MSC donors characterized by Nanoparticle Tracking Analysis showed no differences in the hBM-MSC-EV concentration and size among the 5 donors (1.83 x 10¹⁰ ± 3.23 x 10⁹ /mL), with …
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A study on human IFIT3 modulation of IFIT1 protein stability
… a novel system using tandem mass tag (TMT)-based quantitative proteomics aimed at identifying IFIT1 binding proteins which interact in the absence of IFIT3. Overall, our findings illuminate the mechanism involved in IFIT3 modulation of the protein stability of IFIT1. These findings will contribute …
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Daily organisation of cellular physiology by mTOR kinase
… protein, and functionally characterise them by quantitative proteomics and phosphoproteomics. I find that, in both cultured cells and in mouse liver, soluble protein rhythms are modulated by mTORC activity. Through pharmacological inhibition of mTORC1 and 2 I explore how circadian regulation of …
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