Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"quantitative microscopy"”.
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Long-Term Quantitative Microscopy: From Microbial Population Dynamics to Growth of Plant Roots
<p>Quantitative optical measurements at the micron scale have been crucial to the study of multiple biological processes, including bacterial chemotaxis, eukaryotic gene expression and y development. Extending measurements to long time scales allows complete observation of processes that are …
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Taking shape : the path to myosin activation in the Drosophila ventral furrow
… Drosophila melanogaster ventral furrow. Using quantitative microscopy to measure the pattern of transcription, signaling, myosin activation, and cell shape in the Drosophila mesoderm, I found that cells within the ventral domain accumulate different amounts of active apical non-muscle myosin 2 …
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An experimental investigation of homogeneous fatigue damage in a random short-fiber composite under combined tension-torsion loading
… non-destructive replication and subsequent quantitative microscopy techniques.
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Improved plastid transformation in Marchantia polymorpha
… provide a powerful platform for live-tissue microscopy. In this thesis, tools for Marchantia plastid transformation are systematically improved by the generation of the first plastome sequence assembly for a widely used laboratory strain of Marchantia, optimisation of the transformation …
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Kinetochore Structural Plasticity and its Regulation in <i>Saccharomyces cerevisiae</i>
… I have used a combination of in-house developed quantitative microscopy, genetic techniques and mathematical simulation in the budding yeast, <i>S.cerevisiae</i>, to elucidate a novel structural transition of the kinetochore. During anaphase, the microtubule undergoes rapid depolymerization. …
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The Intracellular Kinetics of HIV-1 Replication
… another. Using a variety of techniques including quantitative microscopy we tracked the timing of these events, both in bulk infected cultures and in single infected cells, and generated a time line of the HIV-1 replication cycle. We find that there is a delay of about 11 hours between integration …
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Study of 3D Genome Organisation in Budding Yeast by Heterogeneous Polymer Simulations
… provides a significantly improved match with quantitative microscopy measurements of telomere positions, the distributions of 3D distances between pairs of different loci, and the mean squared displacement of a labelled locus. The quantified contacts between chromosomal segments were similar …
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The Functional Cellular Identity and Interconversion of Human White, Beige, and Brown Adipocytes
… this gap, we here use an integrated multiomic, quantitative exometabolomic, and 13C-assisted metabolic modelling approach to quantify metabolic differences between human white and brown adipocytes isolated from the same donor. To this extent, we present a novel workflow for the inference of …
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Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells
… alternating pulses. Furthermore, a method for quantitatively measuring the permeability of the cell membrane was proposed and validated. From data near the electroporation threshold, the response of cells varies widely in the manner in which cells become permeabilized. This method is applied to …
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Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… captured by myosin-II in neighboring nodes. Live microscopy shows that Cdc12 localizes to a broad band of 30 to 50 dynamic nodes, where actin filaments are nucleated in random directions. Consistent with the SCPR model, myosin motor activity is required to condense the nodes into a contractile …