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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 54 for “"quantitative Polymerase Chain Reaction (qPCR)"”.

  1. Arousal patterns, fungal loads, and wing damage in a surviving population of little brown bats (Myotis lucifugus)

    Daly, Brooke A.2024

    … load (<em>n</em> = 56) was evaluated through quantitative polymerase chain reaction (qPCR). High autumn temperatures (>10°C) favored growth of the fungus, while mid-hibernation temperature (3.2°C) was unfavorable. The mean torpor bout length (TBL) was similar to bats uninfected by WNS (16.8 …

    emich Repository record

  2. An experimental investigation into the mechanisms of bacterial evolution

    Wan, ZhenmaoThe Graduate School and University Center of The City University of New York2001

    … I will develop a growth assay utilizing the quantitative polymerase chain reaction (qPCR) to provide real-time enumeration of genetic marker abundance within bacterial populations. Second, I will focus on horizontal gene transfer in <em>E. coli</em> occurring through a process called …

    cuny-grad Repository record

  3. Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations

    Hynes, Annette MichelleMassachusetts Institute of Technology2009

    … clade had similar pigmentation. I developed a quantitative polymerase chain reaction (qPCR) method to distinguish between these two clades. In field populations of the Atlantic and Pacific Oceans, the qPCR method revealed that clade II Trichodesmium spp. were more prominent than clade I in the …

    mit Repository record

  4. Persistence of Bacteroides ovatus under simulated sunlight irradiation

    Dong, ShengkunUniversity of Illinois at Urbana-Champaign2014

    … propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, …

    uiuc Repository record

  5. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation

    Tyler, Ronald Dale Jr.Virginia Tech2012

    … were tested with a commercial paratuberculosis quantitative polymerase chain reaction (qPCR) assay and had a sensitivity of 92%. To determine assay specificity, 21 FFPE tissues from animals without gastrointestinal disease combined with 13 FFPE tissues from non-ruminant animals (frog, dove, …

    vt Repository record

  6. Biodegradation of QACs in lake and river water and effects on the microbial community

    Joseph, Frantz2025

    … and generally increased with repeated amendment. Quantitative polymerase chain reaction (qPCR) results showed a higher abundance of several ARGs (qacF, mexB, and sul1) in Mississippi River water than in Trout Lake samples. ARGs also tended to increase in abundance with repeated QAC amendments and …

    umn Repository record

  7. Managing Phomopsis Stem Canker of Sunflower Using Improved Diagnosis and Quantification of the Causal Pathogens

    Olson, Taylor Rae2017

    … of the disease with effective diagnostics, two quantitative polymerase chain reaction (qPCR) assays were designed from the translation elongation factor region (EF1-α) of <em>D. helianthi</em> and <em>D. gulyae</em>. The specificity of the two qPCR assays was validated by examining the assays …

    sdstate Repository record

  8. CaV3.2 Channels and BKCa-Mediated Feedback in Vascular Smooth Muscle

    Hashad, Ahmed MohamedCumming School of Medicine2018

    … pressure myography, immunohistochemistry, quantitative polymerase chain reaction (qPCR) and computational modeling. In initial work, perforated patch electrophysiology was used in concert with Ca2+ imaging to illustrate the coordinated interplay between CaV3.2 and two other Ca2+ permeable …

    calgary Repository record

  9. Metagenomic Analysis of Antibiotic Resistance Genes in the Fecal Microbiome Following Therapeutic and Prophylactic Antibiotic Administration in Dairy Cows

    Caudle, Lindsey ReneeVirginia Tech2014

    … by Illumina paired-end HiSeq sequencing and quantitative polymerase chain reaction (qPCR). Illumina sequences were analyzed using MG-RAST and the Antibiotic Resistance Gene Database (ARDB) via BLAST. Abundance of ampC, ermB, tetO, tetW, and 16S rRNA genes were determined using qPCR. All data …

    vt Repository record

  10. A Framework for Standardized Monitoring of Antibiotic Resistance in Aquatic Environments and Application to Wastewater, Recycled Water, Surface Water, and Private Wells

    Liguori, Krista MargarettaVirginia Tech2023

    … and cefotaxime-resistant Escherichia coli. Quantitative polymerase chain reaction (qPCR)- and culture-based protocols were developed and pilot tested in two independent laboratories on a set of six water matrices: wastewater, recycled water, and surface water from six different wastewater …

    vt Repository record

  11. Spatial Relationship between the Trichloroethylene Degrading Bacteria Dehalococcoides Sp., Sulfate Reducers and Archaea during Reductive Dechlorination

    Gautam, Pujya Wagle2018

    … TCE. The DNA analysis was performed by using quantitative polymerase chain reaction (qPCR) and fluorescent in situ hybridization (FISH). In this study concentrations of Archaea were higher in all reactors than other microorganisms under study. In the type 1 reactor, with increasing …

    wvu Repository record

  12. Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction

    Lopez Nicora, Horacio D.University of Illinois at Urbana-Champaign2010

    … of soybean roots by H. glycines with real-time quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a consistent inoculation method was developed using to provide active …

    uiuc Repository record

  13. Alendronate Alters Single Cell Gene Expression of Cortical Osteoblast Lineage Cells During Bone Loss

    Murphy, Ryan Jakob1970

    … analyze their gene expression profiles using the quantitative polymerase chain reaction (qPCR). The mice used for the study were divided into 4 treatment groups, including ovariectomized mice (OVX) and sham surgical controls (SHAM), treated with or without the anti-resorptive bisphosphonate drug …

    dominican Repository record

  14. Synthetic Functionalized Scaffolds for Culture and Maintenance of Primary Human Hepatocytes

    MacPherson, Douglas2018

    … Analysis of RNA levels combined with 5 quantitative polymerase chain reaction (qPCR) demonstrated an increase in gene expression for hepatocyte-­‐specific markers.</p>

    cuny Repository record

  15. Analysis of the efficacy of a constructed wetland in treating human fecal contamination

    Kondepudi, Kathyayani ShobhnaMassachusetts Institute of Technology2012

    … methods to enumerate indicator bacteria and quantitative polymerase chain reaction (qPCR) method to quantify the human host-specific Bacteroides 16S rRNA genetic marker. Constructed wetlands behave as sinks and transform pollutants to improve the quality of surface runoff. The Alexandra Canal …

    mit Repository record

  16. Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations

    Hynes, Annette MichelleMassachusetts Institute of Technology and Woods Hole Oceanographic Institution2009

    … clade had similar pigmentation. I developed a quantitative polymerase chain reaction (qPCR) method to distinguish between these two clades. In field populations of the Atlantic and Pacific Oceans, the qPCR method revealed that clade II Trichodesmium spp. were more prominent than clade I in the …

    woods-hole Repository record

  17. Investigating Prostaglandin E2 activation of the p38 pathway of oesophageal squamous cell carcinoma

    Rambout, XavierULiège. GxABT - Liège Université. Gembloux Agro-Bio Tech2008

    … profiles were studied over time respectively by quantitative polymerase chain reaction (qPCR) and western blot. The role of p38 was assessed by addition of a specific inhibitor, SB203580, to the cells under investigation. Results and Discussion: It was shown that PGE2 alone could not trigger …

    liege Repository record

  18. Impact of heated passive headspace concentration on DNA recovery from semen in fire-scene evidence

    Li, Jialing2025

    … protection. For unheated swabs with gasoline, quantitative polymerase chain reaction (qPCR) amplification of long-target amplicon signals (T.Large) was undetectable or extremely low, while interpretable STR profiles were still obtained. After 6 hours of heating at 90 °C, gasoline-contaminated …

    bu Repository record

  19. Excretion of Antibiotic Resistance Genes by Dairy Calves

    Thames, Callie H.Virginia Tech2013

    … class I integron gene, intI1, were measured by quantitative polymerase chain reaction (qPCR). No tetC or intI was detected. There was no significant effect of antibiotic treatment on the absolute abundance (gene copies/ g wet manure) of any of the ARG except ermF, which was lower in the …

    vt Repository record

  20. Characterizing the Persistence of Sewage Enterococci in Mississippi Coastal Waters

    Lewis, Kimberly Anne-Marie2018

    … filtration to enumerate enterococci, and Quantitative Polymerase Chain Reaction ( qPCR) to quantify enterococcal DNA. To assess genetic diversity, BOX-PCR fingerprints of sewage and environmental isolates were compared using UPGMA cluster analysis and Simpson's diversity index. To study …

    usm Repository record

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