Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"quadrupole time-of-flight"”.
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Characterization of Molecular Glycerophospholipids by Quadrupole Time-of-Flight Mass Spectrometry
The physical properties of glycerophospholipids (GPLs) are not only determined by the head group (HG), but also by their fatty acid (FA) chains, which affect their distribution and function within membranes in the cell. Understanding the microheterogenity of lipid membranes on a molecular level …
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Probing Base Metal Coordination Complexes Using Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry
… in the liquid phase but very little in terms of the gas phase. The purpose of this research is to further investigate these conditions and learn more about the reactions that can occur using Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry (ESI QToF MS). This research …
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Development of Liquid Chromatography Mass Spectrometry technique for evaluation and differentiation of plant derived isomers
… against various physiological disorders. Most of the plants containing these compounds have been shown to exhibit multiple bioactivities, notably against various diseases which are currently without a cure, for example human immunodeficiency virus (HIV) associated ailments. As in other plants, …
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Top-Down Interrogation And Characterization Of Biological Macromolecules
… has been widely applied in the field of biomolecule identification and characterization. It provides fast, accurate and robust information relevant to the molecular mass, composition and structure of the target analytes.</p> <p>This thesis is focused on the top-down analysis of various …
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Molecular characterization of the lipidome by mass spectrometry
… are all equipped with metabolic pathways capable of producing an assortment of structurally and functionally distinct lipid species. Despite the structural diversity of lipids being recognized and correlated to specific cellular phenomena and disease states, the molecular mechanisms that underpin …
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Matrix-assisted laser desorption/ionization mass spectrometry for the analysis of collected bioaerosols
The goal of this dissertation was to demonstrate collection, detection and identification of microorganisms from bioaerosols using offline matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the analysis of proteins. MALDI-MS intact bacteria techniques were adapted for use …
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Combining chemical with biological oxidation for efficient treatment of chloronitrobenzene in groundwater
… compound widely used in the synthetic production of pharmaceuticals, pesticides, dyes, lumber preservatives, and many other industrial products. CNB has been recognized as a toxic organic contaminant to humans and is recalcitrant to microbial biodegradation in anoxic environments. When receptors …
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Investigation of circulatory and tissue-specific metabolomic biomarkers in valvular heart disease using mass spectrometry
… in this thesis was based on an investigation of the circulatory and tissue specific metabolic biomarkers in patients with rheumatic heart disease (RHD) and degenerative aortic stenosis (AS), compared to matched, healthy controls. The study hypothesis was that cardiac tissue obtained from heart …
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Detection and imaging of pharmaceutical compounds in skin by MALDI-MS.
… have been developed recently for the examination of biological tissue samples. Tandem mass spectrometry has been employed for the detection of pharmaceutical compounds and also mass spectrometric 'images' have been produced which show the spatial distribution of peptides, proteins and drugs in …
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Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry
Liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOF MS) utilizing electrospray ionization was employed to monitor protein expression in Escherichia coli and Shigella organisms. Automated charge state deconvolution, spectral subtraction and spectral mirroring were used to …
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Synthesis, characterisation and application of novel quinones for the detection of latent fingermarks
Identification of an individual through fingermarks is one of the oldest types of evidence in forensic science. A number of techniques are available for the detection of latent fingermarks on porous surfaces; for example 1,2‐indanedione zinc (IND‐Zn), 1,8‐diazafluoren‐9‐one (DFO), and ninhydrin. …
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Novel customisable phases for micro solid-phase extraction and automated biological sample preparation
… (µSPE) technology through the development of a micro-immobilised-enzyme reactor (IMER) and a µSPE immunoaffinity cartridge to alleviate sample preparation bottlenecks caused by conventional ~18-hour digestions. A novel and customisable material was prepared for bio-ligand immobilisation. A …
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Ndma Formation During Chloramination: Temporospatial Distribution Of Ndma Precursors, The Effect Of Lime Softening Treatment, And Precursor Identification Via Non-Targeted Analysis
… much to be understood, however, about the fate of these precursors in natural water and engineered water system. This work mainly addressed the seasonal variations and geographical occurrence of NDMA precursors in rivers, the effect of lime softening treatment on NDMA formation, and the …
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Toxicological Findings in Fatal Road Traffic Accidents in Cape Town: A Pilot Study
… global burden. Toxicological analyses in cases of RTA fatalities are not performed routinely in SA (apart from alcohol analysis), thus understanding the burden of other drug impairment on road traffic deaths is limited. Aim: A prospective toxicological analysis was performed in a cohort of road …
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Deciphering the chemistry of host-parasitic plants interactions using the UHPLC-q-TOF-MS and chemometrics
… in ethnomedicine globally, the parasitic nature of these plants can impact their secondary metabolite composition and thereby, influence their medicinal properties. The main objective of this study was to contrast the metabolome of Viscum combreticola Engl. (African mistletoe) collected from two …
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Isolation, identification, and characterization of antibiotic-producing microorganisms from soil
The emergence and spread of antibiotic resistance in pathogenic bacteria have posed a serious concern in global healthcare. Highlighting a need for novel antimicrobial agents with diverse mechanisms of action to combat current pathogenic threats. Soil-borne bacteria are constantly exposed to …
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A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
… 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins which may be responsible for the synergism seen between cytokine and injury in …
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Innovations in detecting food fraud using mass spectrometric platforms and chemometric modelling
… addition, tampering or misrepresentation of food, food ingredients and food packaging for an economic gain. It is in many cases close to impossible for consumers and players in the food industry to identify. The aim of this thesis was to study whether mass spectrometric platforms, …
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The Activity of Analogs of the Natural Product Dillapiol and Sessamol as Detoxification Enzyme Inhibitors and Insecticide Synergists
In the present thesis, analogs of the plant derived compound, dillapiol, were investigated for their potential as cytochrome P450 inhibitors and insecticide synergists. Dillapiol was chosen as a lead compound because it has a methylenedioxyphenyl (MDP) functionality that serves as a cytochrome P450 …
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APPLICATION OF DATA ANALYTICS AND SPECTROSCOPY ON THE CHEESEMAKING PRODUCTION CHAIN
… techniques were employed to examine the impact of process variables on the food matrix at selected stages of cheesemaking, following the product’s path along its process line. As a first step, the issue of milk coagulation process monitoring through non-intrusive sensors is taken under …
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