Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 288 for “"qRT-PCR"”.
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A study of gene expression in human normal and carcinogenic cell lines using qRT-PCR
The susceptibility of human lungs to carcinogens depends on the metabolic balance between activation and detoxification pathways, though for the tumour to develop depcnds on activation of cell immortalisation pathway. The correlation of these pathways has not been reported. The present study …
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Osteogenesis During Early Healing Around Titanium and Titanium Zirconium Implants: Immunohistochemistry and mRNA Expression of Bone Markers in Miniature Pigs
… reverse transcription polymerase chain reaction (QRT-PCR) is a method that can be utilized to evaluate the early stages of peri-implant wound healing at molecular level by analyzing the level of gene transcription. In addition, the level of protein expression can be analyzed by …
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A Genomic Approach to Identify The Notch Pathway As A Putative Tumor Suppressor In Endometrial Cancer
… up-regulated or down-regulated using qRT-PCR. All of the genes had distinct expression levels depending on the endometrial carcinoma histotype. As a result, they could serve as molecular markers to distinguish between normal endometrium and endometrial cancer, as well as between low …
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An Ex Vivo Model For The Assessment Of Drug Toxicity On The Human Retina
… was characterised using immunohistochemistry and QRT-PCR. The effect of multiple retintoxins was investigated on the human retinal cell lines MIO-M1 (Müller cells) and ARPE19 (RPE cells), and CHQ on the human organotypic retinal culture (HORC) using the LDH and MTS assays. TUNEL, Western blotting …
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Modelling the early development of cartilage and bone in Mseleni Joint Disease using induced pluripotent stem cells
… a collaborator. Pluripotency was confirmed by (qRT-PCR) gene expression analysis of the reprogramming genes OCT3/4, NANOG and SOX2 and immunocytochemical staining for their proteins. In vitro differentiation into the three germ layers was confirmed by ICC. To generate iPSC-derived mesenchymal …
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Development of Techniques for Analysis of the Human Retinal Ganglion Cell Transcriptome: Application to the Role of Calcium in RGC Death in Glaucoma
… mRNA from serial sections of the human retina. QRT-PCR of neuronal markers validated the technique. Global gene expression analysis, using Illumina arrays, compared expression in the retina ganglion cell layer (RGCL) and entire macula (Mac). Immunohistochemistry and QRT-PCR validated gene array …
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Corneal fibrosis therapies and investigations in the canine corna in vitro
… (1-50 µM) and fasudil (1-10 nM) were performed. qRT-PCR, immunofluorescence (IF) staining, cell viability assay, cell proliferation assay, LIVE/DEAD Viability/Cytotoxicity assay, TUNEL assay, and total cell count were performed. Results: A 25 µM lisinopril and 3 nM fasudil dose were safe, …
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A decrease in dkk1, a Wnt inhibitor, contributes to placental and fetal lipid accumulation in an obesity-prone rat model
… (GMT). No. of bitstreams: 6 Appendix B-qRT-PCR Curves.pdf: 4421573 bytes, checksum: e9ba82137f083fa9f50680e7baf8c92c (MD5) Appendix A-Rodent Obesity Models.pdf: 102817 bytes, checksum: d22fc8b17473156234c55d696e75cadd (MD5) Strakovsky_Rita.pdf: 1636618 bytes, checksum: …
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Hypoxic Stress Induced Gene Modulation in Medaka (Oryzias latipes) and Xiphophorus maculatus
… this study, we employed quantitative Real-Time PCR (qRT-PCR) to assess modulated gene expression in four tissues (brain, liver, gill, and fin) of Oryzias latipes and Xiphophorus maculatus. We analyzed six potential gene targets that had previously been shown in microarray studies to exhibit …
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Metagenomic identification and characterisation of emerging and re-emerging viruses causing disease in febrile patients.
… divergent viruses. Through combining targeted PCR-based assays with untargeted metagenomics, this project aimed to detect infections in two cohorts of patients with fever from low-and-middle income countries (Sierra Leone and Ecuador), characterise any viruses identified and pursue further …
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Molecular Analyses of the Soybean Glabrous Mutation (P1) and of Transgenic Lines Designed to Modify Seed Composition
… was confirmed in the soybean sublines by PCR and Southern blot analysis. The dry seeds from the transgenic lines were significantly different than non-transformed plant seeds in that they had statistically significant increases in both average 100 seed weight and protein/oil ratios. These …
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Identification of OATP1B3 as a potential therapeutic target in Recessive Dystrophic Epidermolysis Bullosa Associated Squamous Cell Carcinoma
… cultures. The validation of these genes by qRT-PCR in replicate cultures of RDEB SCC (n=3), non-RDEB SCC (n=3) keratinocytes and normal human keratinocytes as a control, deduced 5 genes to be significantly differentially regulated. Of particular interest, is the over-expression of SLCO1B3 by …
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Mise au point d'une méthode moléculaire pour l'analyse de virucides
… La méthode d’ICC (Integrated Cell Culture) qRTPCR est une méthode moléculaire par laquelle le matériel génétique viral est extrait d’une population de cellule hôte, pour ensuite être amplifié par qPCR en utilisant des amorces capables d’amplifier l’ARNm viral. Ce projet de maîtrise avait …
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Targeting neurodegenerative phenotypes and mitochondrial dysfunction in a Drosophila Melanogaster model of Parkinson’s Disease
… evaluated using Drosophila's survival assays, qRT-PCR, mitochondrial respiration assays, ATP measurements, protein expression analysis, and RNA sequencing. In parallel, human fibroblast cells carrying NUBPL mutations were subjected to cell viability assays, DNA damage and mitochondrial …
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Molecular genetics of abdominal aortic aneurysm
… real time polymerase chain reaction (qRT-PCR). Methods RNA samples were prepared from full thickness aortic walls obtained during open repair of AAA, aortic bypass for AOD and transplant patients for NA. The quality of the RNA was assessed using the Bioanalyzer 2100 (Agilent) and …
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The Impact of Nitrite on Aerobic Growth of Paracoccus denitrificans PD1222
… filter (p ≤ 0.05) at 95% significance in qRT-PCR and a type II microarray transcriptional analysis at 12.5 mM nitrite in batch culture. Additionally, >25 fold expression of the flavohemoglobin was confirmed by qRT-PCR in continuous culture with nitrite. A deletion mutant determined fhb to …
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CONTRIBUTIONS OF SIGMA-B AND PRFA TO STRESS RESPONSE AND VIRULENCE GENE EXPRESSION IN LISTERIA MONOCYTOGENES
… using quantitative reverse transcription-PCR (qRT-PCR). I found that sigmaB is active preferentially under environmental stress conditions, and activity decreases upon internalization of the bacteria by human epithelial cells. Conversely, PrfA is not active under stress conditions, but is …
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The role of muscle and circulatory microRNAs as regulators and biomarkers of muscle function
… The studies in this thesis used a targeted qRT-PCR approach to address the role of muscle regulatory miRNAs in multiple models.
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Loss of basigin expression in the uterus reduces fertility in female mice
… Bsg was confirmed by quantitative real-time PCR (qRT-PCR), immunohistochemistry (IHC) and immunofluorescence (IF) staining. qRT-PCR indicated much lower Bsg mRNA levels in the cKO uteri compared to the controls. BSG was expressed in the luminal epithelial and stromal cells in the control …
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