Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"q-PCR"”.
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Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus
… protocol and was then coupled with Real-time PCR (q-PCR). IMS coupled to q-PCR was able to detect bacterial numbers in less than 3 hrs with a sensitivity of 1,000 CFU/g of oyster homogenate. In the second method anti-hemolysin serum was produced and employed in Direct colony immunoblot (DCI) …
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Effects of Organic and Conventional Agricultural Practices on Soil Microbial Communities and Molecular Detection of Soil Borne Disease
… by quantitative polymerase chain reaction (Q-PCR). Fluorescent in situ hybridization (FISH) was used as an alternative for visual detection of <em>Sclerotium rolfsii </em>through whole cell hybridization. A higher hybridization signal was detected in soil with high <em>Sclerotium</em> DNA …
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Genomic characterization of Six1 function during skeletal muscle differentiation
… and conventional ChIP assay followed by q-PCR.
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The Role of Tau in Epidermal Stratification and Differentiation and its Implications in Skin Cancer
… and protein expression were assessed using RT q-PCR and Western blotting, and changes in cytoskeletal organisation were determined using immunofluorescence. The overexpression of tau was conducted through the generation of stable cell lines with a doxycycline inducible overexpression of tau. The …
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The dual role of lef-2 in DNA replication and late gene expression during baculovirus-replication
… or quantitative polymerase chain reaction (q-PCR) was used to re-examine virus DNA replication by VLD1 and AcORF63260-1, a virus containing the same mutation within lef-2 derived by directed methods. The results showed that AcORF63260-1 DNA replication was comparable to wild type AcMNPV, but …
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Influenza Virus Detection and Surface Decontamination using Ozone Gas
… This study has allowed the development of a Q-PCR assay that can be used to determine the effectiveness of various decontamination methods on Influenza RNA, whilst also allowing the collection of data suggesting Ozone is not an effective decontamination agent against Influenza RNA on metal and …
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Critical factors controlling regrowth of opportunistic pathogens in premise plumbing
… using quantitative polymerase chain reaction (q-PCR). A high occurrence of Legionella (" 69.0%) and mycobacteria (100%), lower occurrence of L. pneumophila (" 20%) and M. avium (" 33.3%), and rare detection of Pseudomonas aeruginosa (" 13.3%) was observed in both systems. Hartmanella vermiformis …
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Expression, purification, and characterization of a novel cysteine-rich silk protein expressed in the tubuliform and aggregate glands of the black widow spider : a thesis
… a new family of proteins.</p> <p>Specifically, Q-PCR analysis revealed that the CRC-5 mRNAs were predominantly expressed in the tubuliform and aggregate glands. Since the aggregate gland manufactures a more complex aqueous solution compared to the tubuliforrn gland, we focused these studies on the …
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Development of Oral Vaccines Against Lyme Disease
… also other types of OspCs. Our positive q-PCR results from bladder, heart and ear. Most importantly, we have shown that antibodies specific to OspC type K do not protect mice from infection when the homologous type of B. burgdorferi is transmitted via ticks harboring also other types of …
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The synergistic interaction between CD20 monoclonal antibodies and Histone Deacetylase inhibitors in B cell Non Hodgkin’s Lymphoma
… LTA, CD69) which were<br/>confirmed using RT-Q-PCR.<br/><br/>This novel combination elicits the induction of apoptosis in vitro, potentially<br/>through regulation of Bcl-2 family proteins and should be tested in a phase<br/>I/II clinical trial.
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Epigenetic Patterns: Process-Driven Or Sequence-Driven?
… quantitative polymerase chain reaction (q-PCR) of the histone modifications H3K4me1/2/3, H3K9me1/2/3, H4K20me1 and RNAPII were analyzed. We found that certain modifications were affected by each sequence addition - some by a notable increase and some by a notable decrease. While more data …
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Skeletal element elongation and interdigital tissue regression in developing bat limbs: a gene expression analysis
… reverse collinear relationship, with absolute q PCR revealing highest expression of Hoxd10. While the coding protein sequence of these genes appeared highly conserved between bats and other mammals, several changes were found in the CsC region of the digit enhancer Prox, some of which were …
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The role of megalin in the transport of aminoglycosides across human placenta
… megalin expression by western blotting and q-PCR (2) The human choriocarcinoma cell line (BeWo cells) were grown on Transwell plates, and then megalin expression and function were assessed. Results: Megalin protein and mRNA expression were confirmed in samples of human placental villous …
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Characterizing methanogen and methanotroph diversity, abundance and activity within the Hampshire-Avon catchment
… real-time polymerase chain reactions (Q-PCR) of pmoA, mcrA genes and next generation sequencing, were applied to characterize methanogen and methanotroph community structure, abundance and activity in the Hampshire-Avon catchment, UK. Soil and river sediments were taken from sites across …
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Limits of detection of Magnaporthe oryzae Triticum pathotype in wheat seed: implications for pathogen dissemination
… of a modified quantitative and conventional PCR assay. Accurate amount mix ratios of MoT colonized ground seeds / non-MoT colonized ground seeds were stablished with different MoT severity and incidence gradient ratios. Disease severity and incidence gradients were verified by end-point PCR …
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Molecular analysis of La Crosse virus transcription and replication in host cells
… studies utilizing a reverse transcription-PCR (RT-PCR) based assay examined co-regulation of LAC virus RNA synthesis with host metabolic activity. A more sensitive real-time quantitative PCR (Q-PCR) based assay was developed during this study to detect and quantitate the LAC virus …
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Steroid signalling in the human ovarian surface epithelium wound healing
… for semi-quantitative (sq) and quantitative (q) PCR revealed expression of both isoforms in hOSE cells; 3β-HSD1 mRNA was expressed at higher levels relative to 3β-HSD2 mRNA in accordance with the preference of this isoform in peripheral non-steroidogenic tissues. Of the cytokines tested, only …
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Whole genome approaches to identify genes involved in early meiosis.
… of selected transcripts were analysed with Q-PCR and found to correlate strongly to those obtained in the microarray. Available meiotic transcriptome data from rice was compared to the wheat data, which enabled the identification of similar sequences, many previously unidentified, which also …
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Studies into the relationship between GPCR43 and BuA-induced effects on colorectal cancer.
… orphan family of G-protein coupled receptors (GPCRs); GPCR41 and 43. Of the two receptors BuA had the strongest effect on GPCR43. Consequently this thesis investigated the possibility that BuA acts to decrease CRC proliferation and induce apoptosis by binding to and activating GPCR43 on CRC …
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Chondrogenic capacities of equine synovial progenitor populations
… phenotypes was assessed by quantitative RT-PCR of chondrocyte-specific genes, DMMB assays for deposition of sulphated glycosaminoglycans (sGAG), an ELISA assay for collagen type II (Coll II) deposition and the alkaline phosphatase (ALP) bio-assay. The results showed that equine synovial …
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