Global ETD Search
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Showing 1 to 4 of 4 for “"pyruvate formate lyase"”.
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Computational Modeling of Catalytic Mechanisms of Glycyl Radical Enzymes
… for any known radical enzyme. The mechanism of pyruvate formate-lyase has been investigated based for the first time on the complete enzyme model. The key new finding is that quenching of the formyl radical is performed by one of the active site cysteines and not by coenzyme A, as previously …
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Structural insight into the assembly of iron-sulfur clusters and their function in radical generation
… for fully activity in vivo. Two structures of pyruvate formate-lyase activating enzyme from Escherichia coli, an Sadenosylmethionine radical enzyme, were also solved by X-ray crystallography, providing the first structure of an activase from this family of enzymes. These structures revealed the …
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Structural and Biochemical Characterization of Glycyl Radical Enzymes Abundant in Mammalian Gut Microbiota
… dehydratase (HypD) and isethionate sulfite-lyase (IslA), from the pathogen and disease-associated bacteria, Clostridioides difficile and Bilophila wadsworthia, respectively. Overall, our work on HypD and IslA enabled us to propose enzymatic mechanisms for these new GREs; we can now take …
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Oxidative stress in anoxic habitats
… Two of these enzymes, fumarase and pyruvate formate lyse (PFL), were reported to be damaged by O2- and molecular O2 respectively. In this study, we show that the third enzyme, pyruvate ferredoxin oxidoreductase (PFOR), is also damaged by molecular O2 itself. Pyruvate dissimilation in …