Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"pyruvate cycling"”.
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Pyruvate Cycling Pathways and Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells
… understood. β-cells have the capacity to flow pyruvate into the TCA cycle via the anaplerotic enzyme pyruvate carboxylase to engage one of several pathways of pyruvate recycling. Previous work demonstrated that pyruvate cycling was correlated with insulin secretion, and that NADPH may be …
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Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.
… secretion. We have found that anaplerosis via pyruvate carboxylase is essential for both phases of glucose-stimulated insulin secretion, presumably via generation of an increased ATP/ADP ratio. Further, we discovered that expression of PDK1 is upregulated in INS-1 832/13 cells cultured at high …
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Exploring Beta Cell NADPH production using Organelle-targeted Apollo-NADP+ Sensors
… Senescence impacts mitochondrial-dependent pyruvate cycling and nicotinamide nucleotide transhydrogenase (NNT) activity, which are important for production of beta cell NADPH to support biomolecule synthesis during cell growth and for the quenching of reactive oxygen species generated during …
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Nonstationary metabolic flux analysis (NMFA) for the elucidation of cellular physiology
… was obtained that indicated a high level of pyruvate cycling, a low flux through the TCA cycle, and high lactate production. We developed computational and experimental tools to assist with the design of flux analysis experiments. We built a simulator that calculates the effect of different …