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Showing 1 to 20 of 22 for “"pyruvate carboxylase"”.
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Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss
Includes bibliographical references (leaves 199-210)
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Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism
Pyruvate carboxylase (PC; EC 6.4.1.1) ostensibly links carbohydrate and lipid metabolism by supplying oxaloacetate (OAA) from the carboxylation of pyruvate. The OAA pool is necessary in maintaining the anaplerotic supply of carbons to the TCA cycle, and to promote the complete oxidation, to CO2, of …
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating enzymes are …
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Metabolic engineering of C. glutamicum for amino acid production improvement
… tools in order to investigate the role of the pyruvate node in cellular physiology. This was achieved by integrating the tools of bioinformatics, recombinant DNA technology, enzymology and classical bioengineering in the context of control and genetically engineered strains of C. glutamicum. …
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Small molecule kaempferol, a novel regulator of glucose homeostasis in diabetes
… Akt and hexokinase activity, but decreased pyruvate carboxylase and glucose-6 phosphatase activity in the liver homogenate without altering their protein expression. Consistently, kaempferol decreased pyruvate carboxylase activity and suppressed gluconeogenesis in HepG2 cells as well as …
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Design, manufacture, and application of DNA microarrays to study gene expression phenotypes of lysine-producing Corynebacterium glutamicum
… over-expression of the key anaplerotic enzyme pyruvate carboxylase and the use of different medium carbon source compositions, both of which have been shown to influence the yields of biomass on carbon and of lysine on biomass. Three different strains of C. glutamicum that were grown on six …
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Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.
… secretion. We have found that anaplerosis via pyruvate carboxylase is essential for both phases of glucose-stimulated insulin secretion, presumably via generation of an increased ATP/ADP ratio. Further, we discovered that expression of PDK1 is upregulated in INS-1 832/13 cells cultured at high …
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Carbon and Deuterium MRI studies of human brain metabolism
… for improved diagnosis. Hyperpolarized (HP) 13C pyruvate and Deuterium Metabolic Imaging (DMI) are two metabolic imaging techniques that allow for the metabolic uptake of the brain to be measured alongside the downstream metabolic processes. The work begins with the implementation of imaging …
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Metabolic network design and engineering in Escherichia coli
… order to improve succinate synthesis. Phosphoenolpyruvate carboxylase and pyruvate carboxylase were coexpressed to drive the carbon flux toward succinate. Competing pathways of succinate synthesis, the lactate and acetate pathways, were also inactivated to increase more carbon flux toward …
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Health Effects of Dietary Fermentable Fiber (Resistant Starch)
… whole grain flour, increases gene expression of pyruvate carboxylase (PCase), and glucose-6-phosphatase (G6Pase). These two enzymes are involved in intestinal gluconeogenesis (IGN) which improves endogenous glucose control. Certain diets can trigger IGN. Goto-Kakizaki (GK) rats (first) and …
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Pyruvate Cycling Pathways and Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells
… understood. β-cells have the capacity to flow pyruvate into the TCA cycle via the anaplerotic enzyme pyruvate carboxylase to engage one of several pathways of pyruvate recycling. Previous work demonstrated that pyruvate cycling was correlated with insulin secretion, and that NADPH may be …
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An assessment of the biotin needs of developing gilts and reproducing sows in a modern production system
… of sow plasma, liver and milk, while sow hepatic pyruvate carboxylase activity was unchanged. Fetal plasma biotin levels were high in pigs from NB females and were increased four-fold when dams received SB diets. Farrowing performance was reduced in W females; however, level of biotin x type of …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… We found citric acid cycle proteins such as pyruvate carboxylase, citrate synthase, as well as beta-oxidation enzymes; cartinine acyl transferase and long-chain enoyl-CoA hydratase up-regulated from 7 through 18 days post differentiation onset. These data indicate TCA up-regulation for …
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Genetic engineering oleaginous yeasts for improved lipid production
… by over-expressing the native acetyl-CoA carboxylase and diacylglycerol acyltransferase genes. Our best strain RT880-AD, derived from IFO0880, was able to produce 16.4±1.1 g/L lipid from 70 g/L glucose and 9.5±1.3 g/L lipid from 70 g/L xylose in shake-flask experiments. Next, in order to …
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Metabolic pathways of Campylobacter fetus
… in BM + 0.5% glucose or 0.5% acetate. No pyruvate dehydrogenase was found in either strain. Pyruvate was carboxylated by pyruvate carboxylase in H840 and by malic enzyme in 482 and PB1. All of them contained the activities of aspartase and glutamate dehydrogenase. Glucose was not …
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Proteomic analysis of genetically stratified grade-I meningioma
… expressions including CLIC3, CRABP2, GMDS, and Pyruvate carboxylase were validated via WB and Simple wes and an increased expression of KLF4K409Q/TRAF7 specific protein Endoglin confirmed via both WB and IHC. NF2-/- mutated meningioma-specific one protein, Annexin-3 showed significant expression …
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Flavonol kaempferol in the regulation of glucose homeostasis in diabetes
… in the liver and skeletal muscle and reduced pyruvate carboxylase (PC) activity and glycogenolysis in the liver. Unlike its effect on T2D mice, kaempferol effect in lean STZ-induced diabetic mice was not associated with changes in plasma insulin levels. In the last study, we found that …
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Insights into substrate utilization and transcriptional regulation in methanosarcina
… presents biochemical and genetic studies on pyruvate utilization in Methanosarcina as a substrate for methanogenesis and the regulation of methylotrophic methanogenesis in M. acetivorans in an effort to further delineate the intrinsic metabolic diversity found in Methanosarcina species. …
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Antidiabetic activity of Schkuhria pinnata – Biological screening, PK analysis and mode of action
… insulin receptor, GLUT-4, glycogen synthase, pyruvate kinase and pyruvate carboxylase expression, suggestive of an insulin mimetic mode of action, while the butanone/pentane extract enhanced adenosine monophosphate-activated kinase (AMPK) protein expression by a non-insulin dependent …
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