Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"pyrrolysyl tRNA"”.
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STUDIES OF THE PYRROLYSYL-TRNA SYNTHETASE
… the 22nd amino acid encoded by the UAG codon. Pyrrolysyl-tRNA synthetase (PylS) is the specific enzyme that can attach Pyl to its cognate tRNA, the amber suppressor tRNAPyl. The biosynthesis of pyrrolysine required the function of three genes, pylC, pylB, pylD, which were identified in the same …
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Expanding the genetic code of a mouse
… proteins(Greiss and Chin, 2011). An aminoacyl-tRNA synthetase and a tRNA are used to specifically insert the unnatural amino acid during mRNA translation, in response to an amber stop codon (UAG) placed at a user-defined site in a gene interest (Davis and Chin, 2012) In this study, I used …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… in mammalian cells in Chapter 3. Using the pyrrolysyl-tRNA synthetase (PylRS)/Pyl tRNACUA system, I showed that unnatural amino acid can be site-specifically incorporated throughout IFITM3 in mammalian cells. The site-specific labeling of IFITM3 with unnatural amino acids has provided new …
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Genetic Code Expansion in Mammalian Cells
… machinery often importing an aminoacyl-tRNA synthetase (aaRS)/tRNA pair that does not cross-react with the endogenous aaRS/tRNA pairs. This approach has allowed for co-translational incorporation of a variety of non-canonical amino acids, including amino acids for photocrosslinking, …
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New chemical and biosynthetic methodologies for the study of lanthipeptides
Recent genome sequencing efforts have revealed that a common biosynthetic route to peptide natural products involves ribosomally synthesized and posttranslationally modified peptides (RiPPs). One of the largest classes of RiPPs is the lanthionine-containing peptides (lanthipeptides), which are …