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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 97 for “"pyrosequencing"”.
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Mitochondrial Dna Analysis By Pyrosequencing
… consuming and labor intensive, on the other hand pyrosequencing is a nonelectrophoretic, rapid, reliable, and sensitive sequencing method which can be easily automated. Therefore pyrosequencing could enable the widespread use of mitochondrial DNA in forensic casework and reduce the amount of time …
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The Use Of Pyrosequencing For The Analysis Of Y Chromosome Single Nucleotide Polymorphisms
… SNP analysis systems: single base extension and Pyrosequencing. Pyrosequencing was determined to be superior to single base extension, due to the wealth of information provided with sequencing and the flexibility of designing primers for analysis. Using Pyrosequencing, 50 Y chromosome loci were …
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COMMUNITY ANALYSIS OF A UNIQUE FULL-SCALE WASTEWATER TREATMENT PLANT AS REVEALED BY 454-PYROSEQUENCING
… treatment plant that may benefit from the use of pyrosequencing techniques is the Los Alamos National Laboratory Sanitary Wastewater System (LANL SWWS) located in Los Alamos, New Mexico. This plant experiences highly variable flow and utilizes glycerin as a supplemental carbon source due to …
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Methylation-Specific Differentiation of Vaginal Epithelial Cells for Forensic Tissue Typing by Bisulfite Conversion and Pyrosequencing
… vaginal epithelial tissue for forensic purposes. Pyrosequencing has several advantages over conventional serological analysis, and the development of a multiplex kit using these markers will aid in the conservation of precious DNA samples that can be used for other forensic purposes.</p>
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Evaluation of bacterial sampling methods for use with the bacterial tag-encoded flexible (FLX) amplicon pyrosequencing (bTEFAP) technique
… sampling and characterization of bioaerosols. Pyrosequencing promises to be a novel, molecular-based technology that is exceptionally sensitive, low-cost, and provides a reasonable turnaround in the identification, distribution and concentration of aerosolized microorganisms. However, …
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Comparative analysis of bacterial and fungal communities in two dairy parlors through the use of pyrosequencing, riboprinting, culture techniques, and microscopic analysis
… to characterize and identify bacteria and fungi. Pyrosequencing, culture techniques, and riboprinting were compared for all of the bacterial samples and pyrosequencing; culture techniques; and microscopic analysis was used to compare the fungal samples. SKC BioSamplers were used to take area …
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Sensitivity and Species Specificity of Bisulfite Modification and Pyrosequencing Technology Used to Identify Tissue-Specific DNA Methylation Patterns in Common Biofluids Foudn in Crime Scenes
… bisulfite modification, PCR amplification, and pyrosequencing, it was concluded that these three markers were sensitive enougn for . forensic casework in varying degrees. The USP49 and DDX4 marker produced adequate sequencing profiles from at least five nanograms of DNA, without a second round …
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Strategien zur HLA-Typisierung mit PyrosequencingTM
… in der molekulargenetischen Praxis dar. Die Pyrosequencing-Technik ist gut geeignet, um kurze DNA-Abschnitte mit weitgehend bekannter Sequenz schnell und effizient zu untersuchen. Ziel dieser Arbeit war die Entwicklung von Pyrosequencing-basierten Methoden zur HLA-Typisierung. Exemplarisch …
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Domestic 'laker' ships as a potential pathway of nonindigenous species in the Great Lakes
… ports, and identified invertebrates through 454 pyrosequencing of 18S rDNA. Ballast samples contained 12 brackish potential nonindigenous species, while St. Lawrence River port samples contained two fresh and 27 brackish species. Québec City poses the greatest establishment risk due to high …
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FUNCTIONAL DIVERSITY OF FUNGI ASSOCIATED WITH DURUM WHEAT ROOTS IN DIFFERENT CROPPING SYSTEMS
… wheat crop was studied using plate culture and pyrosequencing. Pyrosequencing detected more Fusarium spp. in the roots of durum wheat after fungicide-treated chickpea than in non-fungicide treated chickpea. Plate culture revealed that the functional groups of fungi responded differently to …
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Investigating the role of miR-21 in autophagy and VMP1
… and methylation of the region. Secondly, using pyrosequencing to study methylation of this region, and 8 others to develop a diagnostic model for IBD. And thirdly to manipulate miR-21 levels in cell lines to investigate the effects on autophagy and VMP1. RESULTS: Investigating the genetics of …
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Identification of Saliva Using DNA Methylation Analysis for Forensic Use
… polymerase chain reaction amplification, and pyrosequencing. Pyrosequencing is a sequence by synthesis method that provides quantitative methylation data. The level of significance in methylation data between tissues was calculated using SPSS statistical package with a one-way ANOVA and …
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An Assessment of Potential False Positive E.coli Pyroprints in the CPLOP Database
… reproducible fingerprint. By simultaneously pyrosequencing multiple polymorphic loci within the ribosomal operons known as the intergenic transcribed spacers, a reproducible output is obtained, known as a pyroprint, which can be used like a fingerprint to identify that organism. This section …
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The impact of chlortetracycline on Drosophila melanogaster and Aedes aegypti
… µg ml-1 and above. Culturable techniques and 454 pyrosequencing, demonstrated that the microbial diversity of Drosophila was predominantly Acetobacter. Bacterial elimination through egg dechorionation yielded some similar results to chlortetracycline treatment. However, fecundity and life-span was …
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The development of a blood-based test of DNA methylation for the earlier detection of high-grade serous tubo-ovarian cancer
… (on the Illumina® Infinium 450K platform). Pyrosequencing analysis, probe-based quantitative PCR and digital PCR were employed to analyse a selection of candidate DNAme markers. The most promising DNAme marker was analysed in a cohort of matched tissue and plasma samples using an optimised …
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Diversity and spatial distribution of fungal endophytes in a Eucalyptus grandis tree
… bar-coding, or metagenetics, was done using 454-pyrosequencing parallel sequencing of the barcode amplicons of all the fungal isolates present in the plants from the total DNA of the plant. Secondly, conventional DNA bar-coding of was done of fungal endophyte isolates. Isolates of the …
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Examination of HIV-1 diversity and evolution by a bioinformatics approach
… evaluated the utility of ultra-deep pyrosequencing to characterize genetic diversity of HIV-1 gag genes within quasispecies. The results showed that ultra-deep pyrosequencing of amplified HIV genes is a better method than the traditional Sanger-clone-based method in the comprehensive …
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Factors influencing the abundance, community composition and activity states of bacterioplankton from the tidal freshwater James River
… was similar based on TRFLP and 16S rDNA pyrosequencing. Community similarity was lower among rRNA libraries suggesting that variable activity states are prevalent in natural communities. Rare taxa were more likely to be metabolically active and were capable of dramatic growth under …
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From single genomes to natural microbial communities : novel methods for the high-throughput analysis of genomic sequences
… the recently developed, ultra-fast 454 pyrosequencing has dramatically reduced the cost and time requirements per sequenced base pair. Furthermore, novel methods have been developed that enable sequencing of the 99 percent of microbes that are difficult to access with conventional, …
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Exploring the Potential Use of Camelina Sativa as a Biofuel Crop for Nevada
… analysis of C. sativa was performed using 454 pyrosequencing to characterize expressed genes in roots, stems, leaves, flowers, and developing seeds of mature plants subjected to drought, cold and high salinity stress Some contig assemblies corresponding to stress-inducible gene and genes …
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