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Showing 1 to 20 of 25 for “"pyrimidine biosynthesis"”.
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Autogenous transcriptional attenuation of de novo pyrimidine biosynthesis in Bacillus subtilis
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:41:16Z Item is restricted indefinitely.
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Using peptides to examine the interaction interface between Aspartate transcarbamoylase and Dihydroorotase in pyrimidine biosynthesis in Aquifex aeolicus
… third steps, respectively, in <em>de novo</em> pyrimidine biosynthesis. Both enzymes form an active complex (DAC) in <em>Aquifex aeolicus</em>, where loop A of DHOase interacts with a domain of ATCase. The main objective of this work is to determine the function of specific residues of loop A in …
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A Study of the Pyrimidine Biosynthesis Pathway and its Regulation in Two Distinct Organisms: Methanococcus jannaschii and Pseudomonas aeruginosa
Methanococcus jannaschii is a thermophilic methane producing archaebacterium. In this organism genes encoding the aspartate transcarbamoylase (ATCase) catalytic (PyrB) and regulatory (PyrI) polypeptides were found. Unlike Escherichia coli where the above genes are expressed from a biscistronic …
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Structure-Function Studies on Aspartate Transcarbamoylase and Regulation of Pyrimidine Biosynthesis by a Positive Activator Protein, PyrR in Pseudomonas putida
The regulation of pyrimidine biosynthesis was studied in Pseudomonas putida. The biosynthetic and salvage pathways provide pyrimidine nucleotides for RNA, DNA, cell membrane and cell wall biosynthesis. Pyrimidine metabolism is intensely studied because many of its enzymes are targets for …
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Protein-protein interaction between multi-functional protein CAD and protein phosphastase 1 (PP1)
… protein that catalyzes the first three steps in pyrimidine biosynthesis. The cell cycle dependent regulation of pyrimidine biosynthesis is a consequence of sequential phosphorylation of CAD Thr-456 and Ser-1406 by the MAP kinase and PKA cascades, respectively. The mechanism that controls the …
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Pyrimidine Genes in Pseudomonas Species
… studies. It also serves as a reference for pyrimidine gene arrangements in P. aeruginosa. The second part compares the physical, and genetic maps of P. aeruginosa PAO1 with the genome sequence. The final section compares pyrimidine gene arrangements in three species of Pseudomonas. …
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Transcriptional regulation of the Bacillus subtilis pyrimidine biosynthetic operon
… encoding all of the 6 enzymes for de novo pyrimidine biosynthesis. Transcription is controlled by the availability of pyrimidines via a PryR-mediated attenuation mechanism at 3 attenuators within the operon, which are located in the 5$\sp\prime$ leader, the pyrP-pyrP intercistronic, and the …
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INVESTIGATION OF ANTIBIOFILM AND ANTIVIRULENCE ACTIVITY OF 5-FLUOROCYTOSINE IN ESCHERICHIA COLI
… my PhD work, based on previous observation that pyrimidine nucleotide availability can affect the production of biofilm and virulence factors in P. aeruginosa and E. coli, I evaluated fluoropyrimidines 5-fluorocytosine (5-FC), 5-fluorouracil (5-FU), and 5-fluorouridine (5-FUrd) as modulators of …
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Characterizing the oligomeric structure and catalytic activity of the dihydroorotase and aspartate transcarbamoylase from the bacterium, <i>bacillus anthracis</i>
… must rely on <em>de novo</em> nucleotide biosynthesis for survival. The dihydroorotase domain is a key enzyme in pyrimidine biosynthesis and a promising drug target. The genes encoding the dihydroorotase (DHOase) and aspartate transcarbamoylase (ATCase) of <em>Bacillus anthracis</em> …
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Exploring Protoheme IX Farnesyltransferase as an Antimalarial Drug Target
… to ETC-dependent essential processes such as pyrimidine biosynthesis. In this work, we take advantage of modern techniques to explore PfCOX10 as a potential new target of antimalarial compounds. Parasites with conditional knockdown of PfCOX10 are screened for growth against compounds known to …
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Defining the Limits and Mapping the Control Sequences of the Gene, Enhancer of Rudimentary, in Drosophila melanogaster
… unclear it has been shown to be involved in pyrimidine biosynthesis, oogenesis, and the cell cycle. Mutations in e(r) are characterized by low viability and decreased fertility. They have also been shown to enhance phenotypes caused by mutations in two genes involved in neurogenesis, notch …
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Quantitative analysis of the effect of leflunomide on neural crest cell gene regulation during early embryonic development
… Leflunomide’s mode of action is to inhibit pyrimidine biosynthesis, thereby, preventing RNA transcription. Neural crest genes are actively transcribed and like many embryonic stem cells and tumour cells genes undergo an increased level of transcriptional pausing and subsequent elongation …
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Molecular target identification of antimalarial drugs using proteomic and metabolomic approaches
… new therapies are urgently needed. Purine and pyrimidine biosynthesis for DNA and RNA synthesis has been recognized as a source of therapeutic targets. Targeted metabolite profiling has aided in the understanding of several biological processes in the parasite besides drug discovery. Therefore, …
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Elaboration of 4′-Phosphopantetheine Adenylyltransferase inhibitors and Benzoxaboroles as antimalarial agents
… two chemical series, viz. Ugi and cyclohexyl pyrimidine series, was subjected to the medium-throughput screening at the University of Cape Town (UCT)'s Holistic Drug Discovery and Development Centre (H3D). PPAT is a previously unexplored target in P. falciparum, and the current study aimed to …
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Molecular properties of aspartate transcarbamoylase and related enaymes from wheat
… and properties of the first three enzymes of pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPTase), aspartate transcarbamoylase (ATCase) and dihydroorotase (DHOase), in various organisms have been reviewed. The molecular organisation of these three enzymes has been investigated in …
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The regulatory roles of PyrR and Crc in pyrimidine metabolism in Pseudomonas aeruginosa
The regulatory gene for pyrimidine biosynthesis has been identified and designated pyrR. The pyrR gene product was purified to homogeneity and found to have a monomeric molecular mass of 19 kDa. The pyrR gene is located directly upstream of the pyrBC' genes in the pyrRBC' operon. Insertional …
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Nuclear PTEN Regulates Thymidylate Biosynthesis and Cellular Sensitivity to Antifolate Treatment
… for PTEN in controlling de novo thymidylate biosynthesis and may also open novel therapeutic avenues for targeting nuclear-excluded PTEN prostate cancer cells with anti-folate cancer treatment. The first four chapters of this dissertation are introductory information that outlines the role of …
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Characterization of the regulatory domain of the mammalian multifunctional De Novo pyrimidne biosynthetic enzyme CAD
Pyrimidine nucleotides play a central role in cellular regulation. Most cells have two pathways to fulfill their pyrimidine nucleotide pools: the de novo and the salvage pathway. The de novo pyrimidine pathway begins with glutamine, ATP and bicarbonate, and through six-enzymatic steps yields …
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CRISPR-mediated interrogation of small cell lung cancer
… key metabolic pathways, including the de novo pyrimidine biosynthesis pathway. Pharmacological inhibition of Dhodh, a key enzyme in this pathway, reduced the viability of SCLC cells in vitro and strongly suppressed SCLC tumor growth in vivo, validating this pathway as a promising therapeutic …
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