Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"pyocyanin"”.
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Modulation of airway mucin glycosylation by Pseudomonas aeruginosa pyocyanin
… we examined the ability of PA virulence factors pyocyanin (PCN), LPS, flagella and alginate to modulate the levels of sialyl-Lewisx modifications on airway mucins in a mouse model and in in vitro cell culture. We found PCN to be a potent inducer of sialyl-Lewisx in both mouse airways and in the …
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Investigating the role of Leucine-Responsive Regulatory Protein (Lrp) in the production and regulation of specific virulence factors in Pseudomonas aeruginosa
… antibiotic resistance. Virulence factors such as pyocyanin and siderophores contribute to this resistance as they help with virulence and nutrient acquisition. Previously, Lrp has been shown to bind to PQS in <em>P. aeruginosa</em>, a key player in quorum sensing, and when <em>lrp</em> is knocked …
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A cystic fibrosis infection monitor
… of redox active phenazine compounds, including pyocyanin. Slight changes in the impedance were observed with S. aureus and H. influenzae, but were not the same as those observed with P. aeruginosa. This suggests that the sensor could be clinically useful for the detection of P. aeruginosa in …
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Deciphering the mechanism of foxa2 inactivation by respiratory pathogens in chronically diseased lungs
… dominant in CF and advanced stages of COPD. Pyocyanin is a redox-active virulence factors secreted copiously in CF and COPD airways by P. aeruginosa, and is a major inducer of mucus hypersecretion. Among the fungal pathogens, Blastomyces dermatitidis is a causative agent of acute respiratory …
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A New Class of Antibiotic That Prevents Drug Tolerance, Persistence, and Resistance By Controlling Emergence of Phenotypes.
… the swarming and twitching motilities, and pyocyanin and elastase production. Because these phenotypes are controlled by a broad range of signaling pathways, this approach simultaneously control the multiple signaling mechanisms, by which bacteria elude antibiotic treatments. Glycolipid, …
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Ancient Triassic Halite as a novel source of antibiotic chemical diversity
… pSB401, pyoverdine production in PAO-MW1, and pyocyanin inhibition in PAO1. Initial characterisation of the bioactive(s) using TLC-overlays, biochemical tests, and LC-MS/MS analysis excluded structural similarity to known AHL molecules, suggesting that it may be a chemically modified AHL or a …
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Influence of Indole and Mixed Culture Growth on Pseudomonas Aeruginosa Biofilm Structure
… of virulence mechanisms controlling pyocyanin, rhamnolipids, and elastase production, inhibiting quorum sensing.
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Construction of a Pseudomonas aeruginosa Dihydroorotase Mutant and the Discovery of a Novel Link between Pyrimidine Biosynthetic Intermediates and the Ability to Produce Virulence Factors
… produced 40% less of the phenazine antibiotic, pyocyanin, than did the wild type. As both of these compounds have been reported to be vital to the virulence response of P. aeruginosa, we decided to test the ability of the DHOase mutant strain to produce other virulence factors as well. Here we …
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ROLE OF CRC IN THE REGULATION OF ALGINATE IN PSEUDOMONAS AERUGINOSA
… had the expected phenotypes with respect to pyocyanin production, biofilm formation and diauxic growth. When a PalgD-lacZ (translational) fusion was tested, the crc mutant showed increased algD expression as predicted for a mRNA-binding repressor. Another Ptrc-algD-lacZ (translational) …
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The effect of e-liquids and electronic cigarette vapour extract (ECVE) on key respiratory pathogens
… of two PA virulence factors, pyoverdine and pyocyanin. The chemical components of e-liquids components can act as environmental stressors resulting in modification of important bacterial virulence factors. <br/><br/>Exposing respiratory bacteria to ECVE or cigarette smoke extract (CSE) before …
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Surface Enhanced Raman Spectroscopy for Diagnostics in Ocular Health
… most robust signal, particularly for detecting pyocyanin, a key virulence factor in Pseudomonas aeruginosa (PA01) biofilms. The novelty of this work is the application of this optimized protocol to pig cornea samples, which closely mimic human corneal composition. The SERS spectra from the …
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The influence of mucins on bacterial communities
… the anti-staphylococcal effects of P. aeruginosa pyocyanin. In Appendix C, I explore the impacts of mucins on P. aeruginosa quorum sensing, an important pathogenic determinant in P. aeruginosa infections. I found that mucins suppress the expression of P. aeruginosa Las and Rhl quorum sensing genes …
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Consequences of redox-active phenazines on the physiology of the opportunistic pathogen Pseudomonas aeruginosa
… and oxidized endogenous phenazines, including pyocyanin, 1-hydroxyphenazine, and phenazine-1-carboxylic acid. Exogenous oxidants such as methylene blue, paraquat, and 2,6-anthraquinone disulphonate, do not support anaerobic survival, suggesting that phenazine survival is an evolved trait that …
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ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS
… strains. Some of the AI-2 analogs quench pyocyanin (toxin production) in the opportunistic pathogen Pseudomonas aeruginosa. Based on these observations, I used analog cocktails to quench QS en masse in assembled synthetic ecosystems. Finally, I tested the efficiency of the analogs in …
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Legionella pneumophila in Domestic Hot Water Systems: Evaluation of Detection Methods and Environmental Factors Affecting Survival
… Pseudomonas aeruginosa (up to 67.5 cfu/ml) and pyocyanin (up to 9 mg/l) did not have an effect on Legionella recovery under the tested conditions. Environmental factors affecting survival of free living Legionella pneumophila in hot water tanks were also studied. After one day exposure in …
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Understanding the molecular mechanism through which aspirated bile triggers chronic Pseudomonas aeruginosa infections in respiratory disease
… (SNPs) in quorum sensing (lasR) and both the pyocyanin (phzS) and pyomelanin (hmgA) biosynthetic pathways. These mutations have been previously described in various clinical isolates of P. aeruginosa. Loss of Pseudomonas Quinolone Signal (PQS) production in the pigmented variants underpinned …
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The effect of human cytomegalovirus on neutrophil survival, autophagy, and extracellular traps
… structures were stimulated using PMA or pyocyanin, and their release was dependent on the generation of ROS: severely ROS-deficient murine bone marrow neutrophils were unable to generate NETs. However, small amounts of ROS were sufficient for NETs generation, as neutrophils from acute …