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Showing 1 to 10 of 10 for “"purple membrane"”.
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Light-induced currents from oriented purple membrane
… is a report on photocurrent measurements of purple membrane oriented in an polyacrylamide gel. In the first section, the photocurrent measuring system was frequency analyzed from DC to 100 MHz. The optimized conditions that can measure the photocurrent signal in the 70 ms to 32 ns range …
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Photochemical Studies of Rhodopsin, Purple Membrane, and Their Analogues
Rhodopsin and analogues. The absorption spectrum of the bathoproduct of rhodopsin, which contains 11-cis retinal as chromophore, is obtained at 77(DEGREES) K under the condition that on the average less than one photon can be absorbed by each rhodopsin molecule. The spectrum, with (lamda)(,max) at …
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Cation effects on the purple membrane of Halobacterium halobium
… cation is directly related to the blue-to-purple transition of bacteriorhodopsin; whereas, four other divalent cation binding sites involving carboxyl groups on the bacteriorhodopsin surface do not directly affect the color change. The intrinsic pK$\sb{\rm a}$ of the binding of the special …
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Thermodynamic and kinetic studies of purple membrane from halobacterium halobium
… related experiments with the energy tranducing purple membrane from Halobacterium halobium. In the first experiment, we used flash photolysis to measure the effects of solvent viscosity on the kinetics of the purple membrane photocycle, and we analyzed the data in terms of a modified Kramers' …
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Studies on the Structure and Photochemistry of Bacteriorhodopsin (Proton Pumping, Cation Binding, Synthetic Pigments)
… By Bacteriorhodopsin (bR). bR normally has a purple color ((lamda)(,max) = 558 nm), hence the name, purple membrane. Atomic absorption spectrophotometry shows that purple membrane binds approximately 4 Ca('++) and Mg('++) per bR at pH = 6.0. Ca('++) and Mg('++) can be removed by various …
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A Deuterium Nuclear Magnetic Resonance Study of Amino Acid Dynamics in the Membrane Protein, Bacteriorhodopsin (Nmr)
… have biosynthetically incorporated them into the membrane protein, bacteriorhodopsin in the photosynthetic purple membrane of Halobacterium halobium. For L{(beta)-('2)H(,3)} alanine, DL{(gamma)-('2)H(,6)} valine, DL{(beta),(gamma)-('2)H(,4)} threonine, L{(delta)-('2)H(,3)} leucine, …
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Sensitivity-enhanced nuclear magnetic resonance of biological solids
… times. Many biological systems, especially membrane proteins, have relatively short nuclear relaxation times even at 10 K. DNP experiments on fd bacteriophage and purple membrane show that significant sensitivity enhancements can still be obtained on systems with short relaxation times. …
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Solid-state NMR investigations of the bacteriorhodopsin photocycle
Bacteriorhodopsin (bR) is a heptahelical integral membrane protein with a retinylidene prosthetic group from H. salinarum. The protein creates a proton gradient across the cell membrane by harvesting light energy. Proton transfer events can be segregated into transfers between the Schiff base of …
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Molecular dynamics studies of the protein bacteriorhodopsin
… (bR), a 26 kD protein which residues in the purple membrane of the bacterium Halobacterium halobium. Bacteriorhodopsin undergoes a light-driven cyclic process, which pumps protons across the membrane, in order to maintain a proton gradient necessary for ATP synthesis. The cycle is initiated …
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Molecular Dynamics and Quantum Chemistry Studies of the Protein Bacteriorhodopsin
… (bR), a 26 kD protein which residues in the purple membrane of the bacterium Halobacterium halobium. Bacteriorhodopsin undergoes a light-driven cyclic process, which pumps protons across the membrane, in order to maintain a proton gradient necessary for ATP synthesis. The cycle is initiated …