Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"purine nucleoside phosphorylase"”.
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A continuous spectrophotometric assay for phosphate releasing enzymes
… release is coupled to the phosphorolysis of the nucleoside analogue 7-methylinosine catalyzed by purine nucleoside phosphorylase. When this reaction is monitored at 291 nm, the coupled assay can readily detect 10 nmol P$\rm\sb{i}$ released/min. Our method offers advantages over a recently …
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FAMIN in dendritic cells biochemically restrains T cell priming
FAMIN is a purine nucleoside enzyme that was first identified in genome-wide association studies (GWAS) as increasing risk for Crohn’s disease (CD). Defects in the enzyme that result in loss of catalytic activity are the only known monogenic cause of Still’s disease, a severe autoinflammatory …
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Elucidating the Biosynthesis of Nucleocidin, a Fluorinated Natural Product Produced by Streptomyces Calvus
… variant B FADH2-dependent chlorinase. A putative purine nucleoside phosphorylase (orf206) present in the nucleocidin cluster was heterologously expressed as inclusion bodies in E. coli, setting the stage for purification under denaturing conditions. Finally, enhanced production of nucleocidin was …
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The development of hybrid quantum classical computational methods for carbohydrate and hypervalent phosphoric systems
… is applied to a well-known reaction involving purine nucleoside phosphorylase (PNP) and results lead me to conclude that the method shows promise for modelling glycobiological QM/MM systems.
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Developing methods to construct ring pucker free energy hypersurfaces applied to the analysis of glycosidase enzyme catalytic mechanisms
… ribose derivative is phosphorylated by Purine Nucleoside Phosphorylase (PNP) in order to salvage the guanine base. The effect of the PNP protein environment on ring pucker was studied by using FEARCF SCC-DFTB QM/MD non Boltzmann free energy calculations to quantify the pucker change …
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StCKP and potato tuber dormancy
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