Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"purine metabolism"”.
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Acetate Increases Brain Purine Metabolism And Glial Cell Culture Fatty Acid Content: Studies Investigating Glyceryl Triacetate's Therapeutic Mechanism Of Action
… including carbohydrate, protein, and lipid metabolism. Acetate increases the in vivo and in vitro acetylation-state of histones and is associated with altered cytokine balance. However, the effect that acetate supplementation has on brain purine and lipid content remains unknown. Since …
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Glutamine Antagonism and Its Utlity [sic] as a Therapeutic Modality in Cancer
Glutamine metabolism is important in cancer as it fuels the TCA cycle, plays a role in redox homeostasis, and contributes to the production of nucleotides, amino acids, and lipids for survival, making glutamine metabolism a promising target in cancer therapy. The work outlined in this dissertation …
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Metabolism in Vascular Endothelial Cells: Effects of Hyperglycaemia, Pro-Inflammatory Cytokines and Polyphenols
… endothelium by modulating endothelial cell metabolism. Results: First, the effects of high-glucose concentrations, inflammatory cytokines and polyphenols on markers of endothelial cell function in HUVECs were explored. Hyperglycaemic conditions did not significantly affect cell proliferation …
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The Pro-Apoptotic Function of CED-9 Requires a CED-9-CED-4 Interaction
… RNAi against genes that affect either pyrimidine metabolism (pyr-1) or purine metabolism (gmpr-1 and adss-1) similarly allows ABplpappap to survive but that RNAi against R151.2, which affects both purine and pyrimidine metabolism, does not. gmpr-1(n6457) is suppressed by RNAi against R151.2 or …
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Uric acid metabolism in the Dalmatian coach hound
… three characteristic abnormalities of uric acid metabolism, namely hyperuricaemia, hyperuricosuria and increased renal uric acid clearance. These properties are associated with hypoallantoinaemia and hypoallantoinuria. A result of these abnormalities is a high incidence of urate urolithiasis in …
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The in Vivo Roles of the RdgB Enzyme in Escherichia Coli and the Detection of Rare Non-Canonical DNA Precursors
… I have identified several enzymes involved in purine metabolism which appear to contribute to the contamination of the DNA precursor pools with dITP. I also measured the type and extent of DNA precursor pool contamination in rdgB mutants by developing a powerful method for large-scale …
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Functional Investigation of Genetic Determinants of Red Blood Cell Traits
… iron homeostasis (TF, TFR2) and nucleotide metabolism (GMPR, PKLR). To uncover the function of candidate genes in relation to red blood cell development, I established protocols to generate CRISPR/Cas9-based model systems in human erythroid cell lines (K562 and HUDEP-2) and induced …
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Using high throughput data to understand microbes and their interaction with the environment
… differences, including the upregulation of purine metabolism, possibly consistent with an inflammatory response. The results in this dissertation thus offer insights into how the interplay between metabolic activity of human-associated microbes and their surrounding environment plays an …
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Identification and characterization of thiamine biosynthesis, transport, and regulation elements among bacteroides species
… and amino acid biosynthesis, central, and purine metabolism when thiamine was present in Bacteroides thetaiotaomicron. In contrast Bacteroides uniformis and vulgatus show a much more reserved transcriptomic response to exogenous thiamine. In order to build upon these data, we leveraged …
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Species Preference of Viral Deubiquitinating Proteases Toward ISG15 Through Structural and Enzymatic Characterization
… recessive disorder, which causes a defect in purine metabolism resulting in neurological and physiological symptoms. To better understand the causation of disease due to the R303C mutation, the wild type (WT) and the R303C mutant of ADSL were investigated enzymatically and thermodynamically. …
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Metabolomics of Mammalian and Cellular Models of Aging
… consistent with increases in healthspan.</p> <p>Purines are molecules essential for many cell processes, including RNA and DNA synthesis, regulation of enzyme activity, protein synthesis and function, energy metabolism and transfer, essential coenzyme function, and cell signaling. Purines are …
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Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
… synthetase), a key enzyme in microbial de novo purine biosynthesis, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to N5-CAIR. To date, this enzyme has been observed only in microorganisms, and thus, it represents an ideal target for antimicrobial drug development. Here we …
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Oncogenic KRAS drives heterogeneity in metabolic and signalling pathways through genetic and non-genetic mechanisms
… influence the cell signalling, nucleotide metabolism and cell morphology. We show that the G12D and G12V mutants, frequently observed in KRAS-driven cancers, differ significantly from the PDAC-specific G12R mutant. The mutants display allele-specific growth factor signalling, purine …
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Adverse Effects of Asparaginase in Pediatric Patients with Acute Lymphoblastic Leukemia
… common variants in genes critical to purine metabolism and cytoskeleton function were also associated with development of pancreatitis. Our findings are consistent with a mixed genetic architecture underlying serious adverse drug effects, wherein a combination of rare but highly …
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Investigating the genomic, transcriptomic and metabolic landscape of Chlamydomonas reinhardtii in the context of vitamin B12 availability
… biosynthesis as well as starch and sucrose metabolism genes alongside strong upregulation of one-carbon metabolism, heme binding, purine metabolism, protein degradation, deoxyribonucleic acid (DNA) and histone methyltransferase genes and accumulation of homocysteine, is induced in B12- …
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FAMIN in dendritic cells biochemically restrains T cell priming
FAMIN is a purine nucleoside enzyme that was first identified in genome-wide association studies (GWAS) as increasing risk for Crohn’s disease (CD). Defects in the enzyme that result in loss of catalytic activity are the only known monogenic cause of Still’s disease, a severe autoinflammatory …
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Modeling Uric Acid Kidney Stones Disease in D. melanogaster using RNAi and Dietary Modulation
… is known that uric acid is an end metabolite of purine degradation in humans, but in other species from <em>D. melanogaster </em>to lower apes,<em> </em>uric acid can be further metabolized by urate oxidase into readily excreted allantoin. We hypothesize that if urate oxidase enzyme activity and …
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Μελέτη της σωληναριακής μεταφοράς του ουρικού οξέος στην πολυκυστική νόσο των ενηλίκων με τη χρήση των δοκιμασιών πυραζιναμίδης και προβενεκίδης
… load of uric acid, the final product of the purine metabolism. In spite of the fact that uric acid is almost totally filtered in the glomeruli, only 10 % of the filtered quantity is excreted in the urine, because of extensive reabsorption of urate. Many factors affect the tubular transport of …
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The Physiological and Biochemical Understanding of 5’-Amp Induced Deep Hypometabolism
<p>Our laboratory’s ability to induce deep hypometabolism (DH) via an injection of adenosine monophosphate (5’-AMP) followed by placement in an ambient temperature (Ta) around 15oC currently can last about 3-9 hours. While we have insight into how 5’-AMP induced hypometabolism is initiated, it …
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