Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"pulsed-field gel electrophoresis"”.
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Pulsed-field gel electrophoresis subtyping of salmonella isolates from carcasses, lymph nodes and fecal samples of cattle from slaughter facilities in Mexico
Salmonella is one of the leading causes of foodborne illnesses worldwide; however, in developing countries few surveillance programs exist, as a result limited information about the prevalence of this pathogen at pre-harvest is known. In order to identify the relationship between particular …
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Strain identification, viability and probiotics properties of lactobacillus Casei
… fermentation, PCR, gene sequencing and pulsed field gel electrophoresis, 2) to investigate tolerance of Lactobacillus strains in the presence of acid, bile salts and the or bile salt deconjugation, 3) to investigate viability of freeze dried Lactobacillus strains at various temperature …
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Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies
… of H. felis was determined to be 1200 kb using pulsed field gel electrophoresis. A bacterial artificial chromosome (BAC) was used to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic …
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Functional analysis of plastid-encoded genes
… A new experimental protocol, based on pulsed-field gel electrophoresis (PFGE), was established to reliably assess the homoplastomic state of transformed plants. 1. Functional analysis of the ycf9 gene product: The inactivation of ycf9 in N. tabacum as well as in Chlamydomonas …
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… potentials but the plasmids they encode. Pulsed-field gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250 and pREA100, migrate at approximately 400 kb, 250 kb and 100 kb, …
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Virulence potential of enterobacteriaceae isolated from neonatal enternal feeding tubes
… describes the use of DNA finger printing, via pulsed-field gel electrophoresis (PFGE), to determine if the same strains were isolated on different occasions from the NICUs. Therefore indicating whether certain strains have colonised the NICUs leading to increased exposure and risk to the …
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A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… using molecular typing methods, specifically, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior …
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Neonatal enteral feeding tube as loci for Enterobacteriaceae colonisation and risk to neonatal health
… of the same strain. This was achieved using Pulsed-Field Gel Electrophoresis (PFGE). To determine the potential exposure of neonates to these organisms, a range of physiological and virulence related assays were undertaken; heat tolerance to 55°C, biofilm formation, capsule formation and …
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Implications of the ability of Enterococcus spp. to survive the ensiling process and bovine gastrointestinal tract on the risk of bovine mastitis
… isolates of silage and bovine origin using pulsed-field gel electrophoresis patterns from SmaI restrictions. Dendogram analysis showed none of the isolates met or were greater than a 75% genetic similarity and produced a genetically diverse population. Results suggest Enterococcus spp. from …
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Phenotypic and genotypic analysis of intestinal spirochacies
Pulsed field gel electrophoresis of 82 intestinal spirochaete isolates showed specific differentiation of Serpulina pilosicoli and Serpulina hyodysenteriae although considerable heterogeneity was observed, especially amongst S. pilosicoli isolates. In several cases genotypically similar isolates …
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The prevention and diagnosis of central venous catheter-related infection
… surgery patients were characterised by pulsed-field gel electrophoresis (PFGE). The genetic heterogeneity of CoNS isolated from the skin was demonstrated and specific genotypes implicated in catheter colonisation. In addition, phenotypic and genotypic typing techniques were assessed for …
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The Relapsing Fever Spirochete, Borrelia Hermsii, and Complement Regulatory Proteins
… to be a single genetic locus through pulsed-field gel electrophoresis, restriction digest, and hybridization analyses, and all isolates possessing fhbA carry it on a 200 kb linear plasmid, whereas isolates that lack fhbA instead carry a 170 kb linear plasmid.To investigate the …
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Human cases of Staphylococcus pseudintermedius with probable transmission between companion animals to humans in Saskatchewan
… susceptibility/resistance profiles and pulsed field gel electrophoresis. Staphylococcus pseudintermedius infections were identified by either MALDI – TOF MS or Vitek II. Susceptibility testing was manually completed via microbroth dilution methods according to CLSI guidelines. No …
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Gram-positive infections in patients with end-stage renal disease
… determination of macrorestriction profiles with pulsed-field gel electrophoresis. This highlighted horizontal transfer of microorganisms between different patients and the environment. The phenotypic characteristics of these strains were also investigated to determine characteristics that could …
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Histopathological, biological and molecular characteristics of the pathogenic Spiroplasma penaei isolated from the hemolymph of infected Pacific white shrimp, Penaeus vannamei
… of the S. penaei was 1778 Kb, as determined by pulsed-field gel electrophoresis using undigested DNA. Reduction of virulence of S. penaei was not detected in serial passage 24 and 76 isolates but passage 131 isolate was attenuated as indicated by the number of surviving shrimp and histological …
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Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry
… protein expression in Escherichia coli and Shigella organisms. Automated charge state deconvolution, spectral subtraction and spectral mirroring were used to reveal subtle differences in the LC/MS data. Reproducible intact protein biomarkers were discovered based on their unique mass, retention …
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Investigating the genome complexity of Streptomyces clavuligerus
… strains, we established an optimised method of pulsed-field gel electrophoresis, which allowed visualisation of the three giant linear plasmids pSCL2, pSCL3 and pSCL4, as well as chromosomal fragments in order to establish the physical map of the S. clavuligerus genome.;Furthermore, to establish …
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GENOMIC DIVERSITY IN Staphylococcus Aureus
… restriction endonucleases and subjected to pulsed-field gel electrophoresis (PFGE). Thirty-nine distinct genomic macrorestriction patterns (GPs) were identified using <em>Sma</em>I; 29 of these patterns are from methicillin-resistant <em>S. aureus</em> (MRSA).</p> <p>A dendrogram showing …
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Diversity of acinetobacter baumannii isolates from Egypt
… blaOXA-78, blaOXA-94, blaOXA-89/100. Typing with Pulsed-field Gel Electrophoresis (PFGE) showed an overall similarity at only 28.69% between the isolates, with variation in pattern for isolates with similar blaOXA-51-like genes. Typing with Multilocus Sequence Typing (MLST) identified 6 new …
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Phenotypic and transcriptomic consequences of ribosomal DNA copy number variation in Caenorhabditis elegans and Saccharomyces cerevisiae
… functional consequences of rDNA CNV have been largely understudied in metazoans and as such rDNA CNV represents a source of poorly understood genomic variation. I undertook this project to investigate possible functional and transcriptomic consequences of rDNA CNV in an animal model, the nematode …
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