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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"pulsed field"”.
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Pulsed field separation of biomolecules in a nanofluidic filter array
In this work, pulsed electric fields are introduced as a means to enhance separation efficiency of biomolecules in a nanofluidic filter array channel. Separation under pulsed fields was tested using PBR322 DNA, Lambda Hind III DNA, and a sample containing three SDS denatured proteins. Pulsed fields …
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Pulsed field gradient magnetic resonance measurements of lithium-ion diffusion
… of a dipolar refocusing sequence and a strong pulsed magnetic field gradient, a nuclear magnetic resonance method for the direct measurement of the lithium ion self-diffusion coefficient within room-temperature lithium-ion conductors. Magnetic resonance field gradient measurements derive …
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Pulsed field magnetization of composite superconducting bulks for magnetic bearing applications
… rare-earth magnets are limited to a remanent field of 1.4 T, whereas superconducting materials such as YBCO in their bulk form have the extraordinary ability to trap magnetic fields an order of magnitude higher, whilst being very compact. This gives them the potential to increase efficiency …
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Pulsed field of a magnetising coil wound with a solid conductor of rectangular cross-section
… describes a mathematical approach to solving the pulsed magnetic field for an N-turned magnetising coil. This coil is assumed to be made from a copper conductor with rectangular cross-sectional area. The algorithm accounts for each turns physical dimensions and spatial coordinates with respect to …
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Microscopic diffusion measurements with nanoporous materials: complementary benefits of infrared microimaging and pulsed field gradient NMR
… namely infrared (IR) microimaging and pulsed field gradient (PFG) NMR. The dissertation contains mainly five chapters. The first chapter introduces diffusion phenomena in general and concisely explains the importance and the current challenges of the investigation of molecular diffusion …
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Pulsed-field gel electrophoresis subtyping of salmonella isolates from carcasses, lymph nodes and fecal samples of cattle from slaughter facilities in Mexico
Salmonella is one of the leading causes of foodborne illnesses worldwide; however, in developing countries few surveillance programs exist, as a result limited information about the prevalence of this pathogen at pre-harvest is known. In order to identify the relationship between particular …
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The measurement of the diffusion coefficient of water in poly (ethylene oxide) water solutions using a nuclear magnetic resonance pulsed field gradient technique
… solutions as a function of concentration using a pulsed field gradient NMR technique, for values of the concentration of poly-ox in H2O from to 1.2 gm poly-ox/gm. Near zero poly-ox concentration, the slope of the D vs. concentration curve is quite large. As the concentration increases, the slope …
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Stacks of high temperature superconducting tape as trapped field magnets for energy applications
… materials. In particular, trapped field superconducting magnets, where the field is generated by a circulating persistent current in the sample, can create magnetic fields an order of magnitude higher than possible using conventional ferromagnets, thus increasing the power density …
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Strain identification, viability and probiotics properties of lactobacillus Casei
… fermentation, PCR, gene sequencing and pulsed field gel electrophoresis, 2) to investigate tolerance of Lactobacillus strains in the presence of acid, bile salts and the or bile salt deconjugation, 3) to investigate viability of freeze dried Lactobacillus strains at various temperature …
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Polymer dynamics in bulk, adsorbed and solution by NMR methods
… of small molecules in polymer solutions by pulsed field-gradient proton nuclear magnetic resonance (PGSE ¹H NMR). The diffusion of solvents in polymer solutions is investigated. The diffusion of N-N, dimethylformamide in polystyrene is studied as a function of concentration and temperature. …
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Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies
… of H. felis was determined to be 1200 kb using pulsed field gel electrophoresis. A bacterial artificial chromosome (BAC) was used to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic …
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Functional analysis of plastid-encoded genes
… A new experimental protocol, based on pulsed-field gel electrophoresis (PFGE), was established to reliably assess the homoplastomic state of transformed plants. 1. Functional analysis of the ycf9 gene product: The inactivation of ycf9 in N. tabacum as well as in Chlamydomonas …
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Investigation of High-Temperature Superconducting Trapped Field Magnets in a Superconducting Motor
… investigation of magnetisation and cross-field demagnetisation in high-temperature superconducting (HTS) trapped field stacks (TFSs) within a partially superconducting machine, employing a finite element method (FEM)-based modelling approach. The key innovations presented in this work are: …
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… potentials but the plasmids they encode. Pulsed-field gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250 and pREA100, migrate at approximately 400 kb, 250 kb and 100 kb, …
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Virulence potential of enterobacteriaceae isolated from neonatal enternal feeding tubes
… describes the use of DNA finger printing, via pulsed-field gel electrophoresis (PFGE), to determine if the same strains were isolated on different occasions from the NICUs. Therefore indicating whether certain strains have colonised the NICUs leading to increased exposure and risk to the …
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Molecular Mechanism of VanB Phenotype-VanA VRE
… 이용하여 내성유전자의 구조와 염기서열을 분석하였다. 대상 균주의 유전적 연관성은 pulsed-field gel electrophoresis를 시행 후 Bio-Gene software를 이용하여 분석하였다. 결과: 총 39균주 모두 Enterococcus faecium이었고, 이중 28균주가 VanB 표현형 vanA VRE로 확인되었으며, 그 28균주 모두 vanS 도메인의 유전자 변형은 없었다. 28균주의 VanB 표현형 vanA VRE중 26균주에서 vanY유전자의 결실이 관찰되었고, 이 중 13균주에서 vanZ …
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Multiscale Tortuous Diffusion in Anion- and Cation-Exchange Membranes: Exploration of Counterions, Water Content, and Polymer Functionality
… on water transport in AEMs and CEMs using pulsed-field-gradient (PFG) NMR diffusometry. Water diffusion in both AEMs and CEMs exhibit specific trends as a function of water uptake (wt%), indicating morphological similarities across common chemical structures. Furthermore, restricted …
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A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… using molecular typing methods, specifically, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior …
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Neonatal enteral feeding tube as loci for Enterobacteriaceae colonisation and risk to neonatal health
… of the same strain. This was achieved using Pulsed-Field Gel Electrophoresis (PFGE). To determine the potential exposure of neonates to these organisms, a range of physiological and virulence related assays were undertaken; heat tolerance to 55°C, biofilm formation, capsule formation and …
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Cation and Anion Transport in a Dicationic Imidazolium-Based Plastic Crystal Ion Conductor
… means of multi-nuclear solid-state (SS) NMR and pulsed-field-gradient (PFG) NMR. We quantify distinct cation and anion diffusion coefficients as well as the diffusion activation energies (Ea) in this dicationic imidazolium-based OIPC. Our studies suggest a change in transport mechanism for the …
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