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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"pulsed electric fields"”.
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Algae Lysis with Pulsed Electric Fields
… examines a novel method of cell lysis through pulsed electric field (PEF) application that enables cost-effective extraction methods relative to current enzyme and sonication techniques.</p> <p>A theoretical model for cell membrane potential in the presence of electric field was developed, and …
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Diverse Effects of Nanosecond Pulsed Electric Fields on Mammalian Cells
… benefit has lead to the exploration of electric field effects on cells. Current electric field applications include electroporation of the plasma membrane for introduction of drugs, genes, or other macromolecules into cells. Modeling of how these pulsed electric fields affect cells …
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Microdevices for Investigating Pulsed Electric Fields-Mediated Therapies at Cellular and Tissue Level
… of new diagnostic methods and therapies. Pulsed electric fields (PEFs) are a new class of therapies that take advantage of biophysical properties and have proven to be effective in drug delivery and treating several disorders including tumors. While animal models are commonly being used …
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Nanosecond Pulsed Electric Fields Induce a Mitochondria-Independent Apoptosis in B16F10 Melanoma Cells <i>In Vitro</i>
<p>Nanosecond pulsed electric fields (nsPEFs) are ultra-short pulses that induce direct electric field and biological effects that initiate apoptosis. Here the application of ten 300ns pulses ranging in electric fields from 12kV/cm-60kV/cm was administered to determine the effects on B16F10 …
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Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields
… planning algorithms that calculate the induced electric field distribution in the target tissue. An electric field above a certain threshold induces cell death and parameters are tuned to ensure complete tumor coverage while sparing the nearby healthy tissue. IRE studies have shown that the …
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Pancreatic Cancer: Oncomicrobes, Electric Fields, and Fluid Flow
… intracellular compartment. This approach employs pulsed electric fields to achieve controlled permeabilization of host cell membranes through precise modulation of electric field strength. By combining pulsed electric fields with a standard-of-care antibiotic, we achieved greater than 99% …
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High Angular Momentum Rydberg Wave Packets
… are studied. Application of carefully tailored electric fields to low angular momentum, high- n ( n ∼ 300) Rydberg atoms creates coherent superpositions of Stark states with near extreme values of angular momentum, [cursive l]. Wave packet components orbit the parent nucleus at rates that depend …
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Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications
… buffer. The insulating barriers in a fluid-electrical system create a number of unique and interesting challenges from an electrical engineering standpoint. Primarily, they block the flow of DC currents and create a non-constant frequency response which can confound experimental results …
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Modeling cell and tissue electroporation
Large, pulsed electric fields are becoming an increasingly important tool in drug delivery, gene delivery, and apoptosis induction. Nonetheless, much remains unknown about the fundamental mechanisms by which large electric fields interact with cells and tissue, in part because many critical …
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Pulsed field separation of biomolecules in a nanofluidic filter array
In this work, pulsed electric fields are introduced as a means to enhance separation efficiency of biomolecules in a nanofluidic filter array channel. Separation under pulsed fields was tested using PBR322 DNA, Lambda Hind III DNA, and a sample containing three SDS denatured proteins. Pulsed fields …
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<i>In Vivo</i> Murine Melanoma Tumor Responses to Nanosecond Pulsed Electric Field Treatment
<p>High intensity nanosecond pulsed electric fields (nsPEF) were applied to melanoma tumors to observe functional and structural biological changes and to investigate the possible molecular mechanisms responsible. An animal model was set up by injecting B16F10 mouse melanoma cells into SKH-1 mice. …
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Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation
… focal ablation therapy utilizing short, pulsed electric fields to damage cell membranes leading to cell death. The therapy is minimally invasive, involving the insertion of needle electrodes into the region of interest and lasts less than two minutes. Heat sink effects that thermal …
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Engineered Platforms for the Development of Electroporation-based Tumor Therapies
… an alternative to standard treatments, utilizing pulsed electric fields to permeabilize cell membranes to either enhance drug delivery (electrochemotherapy) or induce cancer cell death (irreversible electroporation). Electroporation treatments show promise in the clinic, however, are limited in …
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Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells
… of biological membranes to high- intensity pulsed electric fields. These fields rearrange the membrane structure to enhance the membrane's diffusive permeability, or the degree to which a membrane allows molecules to diffuse through it, is impacted by the structure, composition, and …
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A unified model of electroporation and molecular transport
… termed electroporation. These pores facilitate electrical and molecular transport across cell membranes that are normally impermeable. By applying pulsed electric fields to cells, electroporation can be used to deliver nucleic acids, drugs, and other molecules into cells, making it a powerful …
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Combinatorial Treatments and Technologies for Safe and Effective Targeting of Malignant Gliomas Using High-Frequency Irreversible Electroporation
… treatments. By utilizing high amplitude pulsed electric fields, IRE is able to permeabilize cells, disrupt the BBB, and induce non thermal ablation of soft tissues. However, IRE is oftentimes accompanied by undesirable secondary effects such as muscle contractions, complex anesthetic …
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Investigating the ablative and immunomodulatory effects of high frequency irreversible electroporation on osteosarcoma in-vitro
… technique that induces cell death by applying pulsed electric fields in targeted regions, potentially triggering an anti-tumor immune response that could also target and prevent metastases. Such a dual functionality of H-FIRE is uniquely suited to treat pulmonary metastatic OS. The goal of this …
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An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies
… and administering a sequence of short, intense, pulsed electric fields (PEFs). The application of these PEFs results in an increase in the transmembrane potential of all cells within the electric field above a critical value, destabilizing the lipid bilayer of the cellular membrane and increasing …
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Advancements in the Treatment of Malignant Gliomas and Other Intracranial Disorders With Electroporation-Based Therapies
… muscle contractions and is susceptible to electrical heterogeneities. Second generation High-frequency IRE (H-FIRE) utilizes bursts of bipolar pulsed electric fields on the order of the cell charging time constant (~1 μs) to ablate tissue while reducing nerve excitation, muscle contraction, …
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Nanosecond Pulsed Electric Field Induction of Programmed Cell Death Is Cell Type Dependent: An <i>in vitro</i> Study
<p>Nanosecond pulsed electric fields (nsPEFs) present a novel and effective method for cancer ablation by eradicating the ubiquitous cancer hallmark of apoptosis evasion and enforcing cancer programmed cell death. To develop nsPEFs as an anticancer method, a comprehensive understanding of cell …
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