Global ETD Search
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Showing 1 to 11 of 11 for “"pulsed EPR"”.
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Investigation of the quinone sites in reaction centers from Rhodobacter sphaeroides by pulsed EPR and spectral simulations
… for study by electron paramagnetic resonance (EPR). In this thesis, pulsed EPR is used to explore the magnetic interactions between the semiquinones and the protein environment, the solvent, and even within the quinone molecule itself. The magnetic coupling constants that characterize these …
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The Binding Pockets of QA and QB in the Photosynthetic Reaction Center of Rba. sphaeroides Probed by Pulsed EPR
… for their binding pockets to be studied with EPR (electron paramagnetic resonance) spectroscopy. Using the pulsed EPR technique, ESEEM (electron spin echo envelope modulation) the interactions between the reaction center semiquinones and their environment were studied for wild type and a …
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Transient and Pulsed Electron Paramagnetic Resonance Spectroscopy on Type I Photosynthetic Reaction Centers
Two time-resolved EPR techniques, have been used to study the light induced electron transfer(ET) in Type I photosynthetic reaction centers(RCs). First, pulsed EPR was used to compare PsaA-M688H and PsaB-M668H mutants of Chlamydomonas reinhardtii and Synechosystis sp. PCC 6803.The out-of-phase echo …
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Characterisation of the (H3-H4)2-tetramer and its interaction with histone chaperones
… dimer. Using targeted protein crosslinking and pulsed EPR we determine that, unlike Asf1, the Nap1 family of histone chaperones can bind H3-H4 in their tetrameric conformation, analogous to that observed within the nucleosome. The ability to bind H3 and H4 as a tetramer has implications in the …
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EPR and solid-state NMR studies on the mechanism of cytochrome bo3 ubiquinol oxidase from escherichia coli
… at the QH-site of these samples were analyzed by pulsed EPR spectroscopy. Selective 15N labeling has revealed the Nε of R71 in the wild type cytochrome bo3 and the Nε of H75 in the D75H mutant cytochrome bo3 as the nitrogen atoms that are strongly coupled with the carbonyl oxygen-1 of the …
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High-frequency time domain electron paramagnetic resonance : methods and applications
… (high field) electron paramagnetic resonance (EPR) spectroscopy. Two of the most important are improved sensitivity and the improved resolution of field dependent interactions. In addition, there are many attractive features to time domain spectroscopy. Pulsed EPR allows for the design of …
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The Application of Pulsed Electron Paramagnetic Resonance to the Investigation of Protein Structure
Pulsed electron-electron double resonance (PELDOR) is a pulsed electron paramagnetic resonance (pulsed EPR) technique used for measuring distances and on rare occasions orientations within biomolecular structures. Site directed spin labelling (SDSL) has provided a method for introducing EPR active …
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Investigation of DNP mechanisms : the solid effect
… to nuclear polarization, via application of pulsed or continuous-wave microwave irradiation. This results in increases in NMR sensitivity of 2-3 orders of magnitude. DNP greatly reduces experimental times and makes some experiments possible that are otherwise unfeasible due to lack of …
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Tat signal peptide recognition during protein maturation and export
… residues R6, K10 and A17 interact with NapD. Pulsed EPR spectroscopy on the isolated signal peptide indicated structural changes of the NapA signal peptide between NapD bound and unbound states, though it was believed that an overall a-helical structure was maintained. Co-purification studies …
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Characterizations on bacterial photosynthesis and respiration with optical spectroscopy and magnetic resonance
… in the LM dimer have been characterized with pulsed electron paramagnetic resonance (EPR, Chapter 3). Both nitrogen data and hydrogen data are consistent with elongated hydrogen bonds to the semiquinone. The binding conformation of QA- is heterogeneous in the LM dimer, with more flexibility on …
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Qo site of the bc1 complex; unlocking the gate of the electron transport chain
… states by ob-serving the paramagnetic species by EPR. Spectral analysis using CW and pulsed EPR shows a SQo species not previously characterized, and has allowed resolution of some interesting struc-tural details. These suggest a species with spin coupling both to neighboring paramagnetic states, …