Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"pulse field gel electrophoresis"”.
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Microbial degradation of high molecular weight polycyclic aromatic hydrocarbons
… of the three strains was possible by pulse field gel electrophoresis and DNA:DNA hybridisation methods. The St. maltophilia strains had similar degradative profiles to community five.
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… equivalent to 0.2 - 20 Gy of IR, as detected by pulse-field gel electrophoresis, accompanied by dose-dependent H2AX phosphorylation at ser-139 and ATM phosphorylation at ser-1981, indicating ATM activation. Furthermore, BrdU photolysis increased phosphorylation of Chk2 (at thr-68) and p53 (at …
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The frequency and characterization of streptococci in aerobic vaginitis (AV) and its association with pregnancy outcomes
… predominant serotype, followed by serotype III. Pulse field gel electrophoresis (PFGE) profile analysis for both GBS and E. faecalis yielded a total of 24 restrictions profiles for GBS and 16 for E. faecalis. Multivariable analysis revealed that parity, gravidity, vaginal discharge, urinary tract …
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The Microbiome of Cystic Fibrosis Sputum and its Association with Incident Lower Airway Infections
… bacterial bioburden. Strains were typed using pulse-field gel electrophoresis to differentiate strain types and distinguish incident infection episodes. Results from this work showed that the microbial community of pwCF that eventually developed P. aeruginosa, or S. maltophilia incident …
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The development of tools to allow genetic and genomic analysis of Frankia
… sizes of the three strains were determined by pulse-field gel electrophoresis (PFGE) and estimated to be 5.5Mb for Ccl3, 9.1 Mb for EAN1pec and 8.5Mb for Eul1c. Several members of the actinomycetales have linear chromosomes; however, our experiments on topology show that the Frankia chromosome …
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The role of recombinational repair in the mechanism of killing during thymine starvation in Escherichia coli
… during TLD using physical methods, such as pulse-field gel electrophoresis to measure levels of double strand breaks. I next investigated the role of major DNA repair and recombinational repair pathways by studying the effect of their inactivation on survival during thymine starvation. …
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Movement and Stability of the Staphylococcal Cassette Chromosome mec among Staphylococcus aureus
… SCCmec type I to recipient strains of S. aureus. Pulse-field gel electrophoresis and mec-associated dru typing were used to confirm the identity of the transductants. Transfer of mecA via transduction occurred at low frequency and required extended selection times for mecA gene expression as well …
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Indagine sulla presenza di Helicobacter pullorum in allevamenti avicoli italiani
… different farms were submitted to genotyping by Pulse-Field Gel Electrophoresis (PFGE). In order to assess the molecular basis of fluorquinolone resistance in H. pullorum, gyrA of H. pullorum CIP 104787T was sequenced and nucleotide sequences of the Quinolone Resistance Determining Region (QRDR) …
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Genomics and host response of S. Typhi infections
… epidemiology of Colombian S. Typhi, based on pulse field gel electrophoresis (PFGE) patterns of S. Typhi DNA using isolates collected throughout the country between 2012 and 2015. This is followed by further analysis of a second data set using isolates collected between 1997 and 2018. Here, …
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Analysis of the S. pombe sister chromatid cohesin subunit in response to DNA damage agents during mitosis.
… and analysis of chromosome integrity by pulse-field gel electrophoresis (PFGE). The northern blot experiments indicated that rad21+ expression levels increased with higher concentrations of MMS, and cell viability decreased at concentrations above 0.1%. The induction of rad21+ …