Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"pseudovirions"”.
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Pseudovirions in the Study of Papilloma- and Polyomaviruses
… virus stocks of these viruses, so-called pseudovirions that morphologically and immunologically resemble native virions but lack the viral genome can be produced in vitro. In the present thesis, we produced such pseudovirions and used them to i) delineate the importance of hypervariable …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… vaccine production and testing are needed. HPV pseudovirions (PsVs) are synthetic viral particles that are made up of the L1 major and L2 minor HPV capsid proteins and encapsidate up to 8Kb of pseudogenome DNA without the need of an encapsidation signal. HPV PsVs are used to test neutralising …
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
… visualize the conformation changes to HPV16 pseudovirions (HPV16 PsVs) resulting from cleavage of L2 by exogenous furin using cryoelectron microscopy (cryo-EM). At 5 Å resolution, we observed that furin-cleaved HPV16 PsVs capsids display widespread changes in the arrangement of capsomeres …
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Working towards a plant-produced Bovine papillomavirus vaccine: expression of BPV1 virus-like particles and pseudovirions in Nicotiana benthamiana
… as safe and efficacious vaccine antigens, and pseudovirions (PsVs) as experimental vaccines and vectors for therapeutic agents. Prophylactic VLP-based candidate vaccines against BPV types 1, 2, and 4 have previously been produced, yet these produce antibodies which are largely type-specific, …
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HPV pseudovirion production in plants
… as virus-like particles (VLPs) and more recently pseudovirions (PsVs), has allowed for unprecedented insights into the lifecycle and immunology of this virus. This has led to the development of two currently available vaccines, namely Cervarix™ and Gardasil®. Cervarix offers protection against …
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The role of surfaceant protein A in immunity to HPV16 pseudovirus infection
… determine whether SP-A and/or SP-D bind to HPV16 pseudovirions (HPV16- PsVs). SP-A was shown to bind to HPV16-PsVs as well as enhance viral uptake by RAW264.7 murine macrophages, while SP-D bound HPV16-PsVs weakly and had no effect on viral uptake. To confirm these observations and to assess …
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Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus
… of VLPs suggests the possibility of developing pseudovirions. This is the first reported replication of BFDV in tissue culture, and will greatly expand the scope of available research.
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Characterisation of surfactant protein a as a novel prophylactic means against oncogenic HPV infections
… as a novel molecule capable of recognising HPV16 pseudovirions (HPV16-PsVs) with functional consequences for reduced infection in a murine cervicovaginal HPV challenge model. Building on these findings, our aim was to assess SP-A's suitability as a novel broad-spectrum HPV targeting molecule to …
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The Influence of HIV-1 Subtype C LTR Genotype on Latency Potential
… HIV-1-expressing vector (pRGH) used to generate pseudovirions following HEK293T transfection. Pseudoviruses harboured a gag-eGFP gene under the control of the participant LTR, allowing measurement of active replication, and an mCherry gene under the control of a constitutive CMV promoter allowing …
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Immunogenic assessment of plant-produced Human papillomavirus type 16 chimaeric L1:L2 virus-like particles and the production of an encapsidated therapeutic DNA vaccine candidate
… a novel E7 DNA vaccine delivered by plant-made pseudovirions (PsVs). A geminivirus-derived self-replicating plasmid encoding a shuffled E7 (E7SH) sequence that has no transformation ability but contains natural cytotoxic T-lymphocyte epitopes, was constructed using Goldenbraid technology and …
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The Immunogenicity of Plant-produced Human Papillomavirus (HPV) Virus-like particles (VLPs) in Mice
… were able to successfully neutralize homologous pseudovirions (PsVs) in PBNAs. Sera from mice immunized with plant-produced VLPs were further tested against heterologous HPV 6, 16, 18, 31, and 45 PsVs. However, none of the tested heterologous HPVs were neutralized, suggesting that plant-made VLPs …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… (APCs). This limitation can be overcome by using pseudovirions (PsVs) as vaccine delivery vehicles. HPV PsVs are highly immunogenic synthetic viral particles consisting of L1 and L2 capsid proteins, which self-assemble to package pseudogenome DNA. Pre-existing immunity to high-risk HPVs through …
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Generation and characterization of HIV-1 subtype C candidate vaccines that will induce high titre antibody responses to HIV-1 envelope glycoprotein
… autologous Tier 2 neutralisation of CAP256_SU pseudovirions. The envelope glycoprotein sequence was modified so as to replace the native leader sequence with the tissue plasminogen activator leader, the furin cleavage site with a glycine rich flexible linker, and to introduce an I559P mutation. …