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Showing 1 to 10 of 10 for “"pseudouridylation"”.
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"fine-tuning" of ribosomal structure and functions by pseudouridylation and rna-protein interactions
… of ribosomal structure and functions by pseudouridylation and RNA-protein interactions</p> <p>by</p> <p>JUN JIANG</p> <p>AUGUST 2012</p> <p>Advisor: Prof. John SantaLucia Jr.</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> <p>Ribosomal structure and …
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STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF ARCHAEAL BOX H/ACA RIBONUCLEOPROTEIN INVOLVED IN RIBOSOMAL RNA PSEUDOURIDYLATION
… ribonucleoprotein complex in sRNP-mediated rRNA pseudouridylation. Certain Ys in rRNAs are evolutionarily conserved from Bacteria to human. Among those, two Ys are present in helix 69 of rRNA and one in helix 90. We successfully deleted Cbf5 in Haloferax volcanii, a haloarchaeon, and showed that …
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High Throughput Detection of Pseudouridine: Caveats, Conundrums, and a Case for Open Science
… of uridine to pseudouridine (Ψ), known as pseudouridylation, is the most abundant post-transcriptional modification of stable RNAs. Due to technical limitations in pseudouridine detection methods, studies on pseudouridylation have historically focused on ribosomal RNAs, transfer RNAs, and …
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Transcriptome-wide pseudouridine profiling reveals modification of critical E. coli mRNAs
… the possible functional consequences of mRNA pseudouridylation, and discuss the potential roles of Ψ in pilus expression in greater depth. Additionally, since I discovered sparse but reproducible modification by RluC and RluD, I compare the known structure and specificity of these three mRNA:Ψ …
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Biochemical and structural studies of human telomerase regulation
… 2018). H/ACA RNPs also have a general role in pseudouridylation of spliceosomal and ribosomal RNAs (Charette and Gray, 2000). The telomerase catalytic core, on the other hand, is responsible for telomeric DNA extension and telomerase recruitment to telomeres (Egan and Collins, 2010; Nandakumar …
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NANOPORE SEQUENCING AS A NEW TOOL TO EXPLORE COMPLEX TRANSCRIPTOMES
… In parallel, we identified putative pseudouridylation sites on multiple sgRNAs, one of which was located in a well-known regulatory region and proposed to have a role in viral subgenomic RNAs translation. Furthermore, within the FANTOM6 consortium, we used an adaption of the cDNA-PCR …
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Platination Kinetics: Insight Into Rna-Cisplatin Interactions As A Probe For Rna Microenvironments
… of active cisplatin complexes. Structure, pseudouridylation, availability of active cisplatin species, and cation/Pt+ electrostatic competitions all impact platination of the two H69 RNAs. Probing neomycin-H69 interactions by platination kinetics indicated that structural changes in …
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The role of RNA modifications in mitochondrial translation
… is also present in mtRNAs. Investigation of pseudouridylation in mitochondria yielded the identification of four novel pseudouridine synthases: PUSL1, RPUSD3, RPUSD4 and TRUB2. Using targeted (RT-PEx) and transcriptome-wide (Psi-Seq) techniques PUSL1 was identified as the enzyme responsible …
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Protein factors involved in the biogenesis of the mitochondrial ribosome
… base methylations, 2’-O-ribose methylations and pseudouridylation. However, the exact function of these modifications is unknown. Many mitoribosome biogenesis factors still remain to be identified and characterised. This work aims to broaden our understanding of two proteins involved in …