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Showing 1 to 20 of 29 for “"pseudouridine"”.
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Biochemical and Structural Studies of Pseudouridine 55 Synthase
Sequence alignment of the families of PsiS identifies the conserved aspartic acid discussed previously as the strictly conserved amino acid. Structural alignment identifies the conserved tyrosine, which is part of hydrophobic core in the active site. The role of the conserved tyrosine is less …
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Transcriptome-wide pseudouridine profiling reveals modification of critical E. coli mRNAs
Pseudouridine (Ψ) is an ubiquitous RNA modification, present in the tRNAs and rRNAs of species across all domains of life, and is now known to be present in the mRNAs of diverse eukaryotes. However, the modification was yet to be identified in bacterial mRNAs, and its functional roles remain …
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High Throughput Detection of Pseudouridine: Caveats, Conundrums, and a Case for Open Science
<p>The isomerization of uridine to pseudouridine (Ψ), known as pseudouridylation, is the most abundant post-transcriptional modification of stable RNAs. Due to technical limitations in pseudouridine detection methods, studies on pseudouridylation have historically focused on ribosomal RNAs, …
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Inosine-containing mRNA induces an innate immune response and is translated with lower efficiency
… We also generated mRNA sequences that contained pseudouridine, an RNA modification known to decrease immune response to in vitro transcribed (IVT) mRNA and elevate the expression of the encoded gene, to examine the interaction between pseudouridine and inosine modifications. While A-to-I editing …
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STRUCTURAL AND FUNCTIONAL STUDIES OF ARCHAEAL BOX C/D GUIDE RNA AND ROLE OF A PUTATIVE HUMAN PSEUDOURIDINE SYNTHASE, PUS10 IN APOPTOSIS
… longer spacer length) than regular Archaeal one. Pseudouridine synthase 10 (Pus10) is the most recently identified Ψ synthase, found only in higher eukaryotes and Archaea. Archaeal Pus10 produces either tRNA Ψ55 or both tRNA Ψ54 and Ψ55 modifications. In Human, its Ψ synthase activity is not yet …
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STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF ARCHAEAL BOX H/ACA RIBONUCLEOPROTEIN INVOLVED IN RIBOSOMAL RNA PSEUDOURIDYLATION
… One of the highly abundant rRNA modifications is pseudouridine (Y) formation. In Archaea and Eukarya, pseudouridine synthases, with the help of small RNAs, form pseudouridines at functionally important regions in rRNA. Cbf5, the pseudouridine synthase, three other core proteins, and a box H/ACA …
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Platination Kinetics: Insight Into Rna-Cisplatin Interactions As A Probe For Rna Microenvironments
… helix 69 (H69) constructs, modified H69 (with pseudouridine) and unmodified H69 (without pseudouridine), and the 790 loop, which has an identical size and nucleotide composition to unmodified H69, were used. Prior to kinetic studies, cisplatin targets on each RNA were determined using RNase T1 …
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The role of RNA modifications in mitochondrial translation
… complex and unfolded interfacial rRNA. Pseudouridine (Ψ), the most common RNA modification found in cells, is also present in mtRNAs. Investigation of pseudouridylation in mitochondria yielded the identification of four novel pseudouridine synthases: PUSL1, RPUSD3, RPUSD4 and TRUB2. …
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Mining and Manipulation of Antibiotic Biosynthesis in Streptomyces
… of two of them were verified. One of these, a pseudouridine oxidase, also showed activity against uridine. Consistent with this finding, feeding of uracil to a mutant S. albus lacking the pseudouridine synthase-like gene truD gave production of the novel N-linked analogue of PUM, suggesting …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… and rich in modified nucleotides, including pseudouridine () and 3-methylpseudouridine (m3) was chosen as a potential target. Helix 69, which is located in domain IV of Escherichia coli 23S ribosomal RNA (rRNA), undergoes conformational changes when in close proximity to the decoding region …
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Investigation of the higher order structure of the spliceosomal RNA network
… prä-mRNA Spleißen. Wir können zeigen, daß drei Pseudouridine und fünf 2'-O-methyl Gruppen im Bereich der ersten 20 Nukleotide der U2 snRNA, nicht aber die m3G Kappe, für effizientes prä-mRNA Spleißen notwendig sind.Im zweiten Teil dieser Arbeit konnte mittels Hydroxylradikal-Kartierung gezeigt …
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The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
… catalyse the methylation of guanosine 1639 and pseudouridine 1248 residue of the 18S rRNA and they are known as WBSCR22 and EMG1. Both proteins are key factors for ribosome biogenesis and previous studies have demonstrated that in this context, they both act as scaffolds and that their catalytic …
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The chemistry and evolution of enzyme function: isomerases as a case study
… chemistry such as oxidosqualene cyclases and pseudouridine synthases from chemically complex sub-subclasses like intramolecular transferases acting on \other groups" (EC 5.4.99) might also improve the classification. An overview of the evolution of isomerase function in superfamilies revealed …
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Isolation And Analysis Of Peptides Binding To Helix 69 Of E. Coli 23 S Rrna From M13 Phage Display
… complex revealed that the presence of all three pseudouridine residues positively contributed towards binding of this peptide to H69. Consecutively, to learn about the role of individual pseudouridines at position 1911 and 1915 towards binding of the peptide, the ESI-MS experiments were performed …
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