Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"proximity ligation assay"”.
-
A role for focal adhesion kinase in the contraction of gastric fundus smooth muscle evoked by cholinergic neurotransmission
… we developed smooth muscle tissue in situ proximity ligation assay (PLA) for the quantitative PLA analysis of protein-protein interaction and protein phosphorylation (Chapter 3). From these studies, we revealed a novel role of FAK in gastric fundus smooth muscle contractile regulation by …
-
Autonomous and non-autonomous regulation of chromatin structure during cellular senescence
… group A (HMGA). Using ATAC-sequencing (assay for transposase accessible chromatin) we demonstrate that nucleosome positioning is substantially altered in RIS and that this re-distribution is also antagonised by NOTCH1, resulting in a distinct chromatin landscape. Importantly, normal or …
-
Investigating the role of ULK1 kinase activity during autophagy
… mechanisms regulating this process I performed a proximity ligation assay (BioID) using MRT68921 as a tool to enrich for autophagy regulators trapped on stalled autophagosomes. With this approach ATG2B was identified, among others, as a protein that is enriched on autophagosomal structures upon …
-
The identification and characterisation of proteins interacting with SUMO-ubiquitin hybrid chains
… are then evaluated in an in vivo context. A proximity ligation assay was developed to probe the association between SUMO, ubiquitin and RAP80, suggesting a tight association between the three after DNA damage inducing stimuli. Interesting, the SUMO targeted ubiquitin E3 ligase RNF4 was shown …
-
THE ROLE OF E6 PROTEIN IN HUMAN PAPILLOMAVIRUS ASSOCIATED UPPER AIRWAY DISEASES
… E6 protein by using co-immunoprecipitation, proximity ligation assay, and confocal immunofluorescence staining. SAMD9 is a host restriction factor for myxoma virus and vaccinia virus. We hypothesize that SAMD9 may have an anti-viral effect against HPV-11, which is still to be determined as we …
-
THE ROLE OF E6 PROTEIN IN HUMAN PAPILLOMAVIRUS ASSOCIATED UPPER AIRWAY DISEASES
… E6 protein by using co-immunoprecipitation, proximity ligation assay, and confocal immunofluorescence staining. SAMD9 is a host restriction factor for myxoma virus and vaccinia virus. We hypothesize that SAMD9 may have an anti-viral effect against HPV-11, which is still to be determined as we …
-
Regulation of Astrocyte Functions by a Parkinson's Disease Gene, DJ-1
… 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. Small interfering RNAs (siRNA) 24 18. Prostaglandin D2 (PGD2) ELISA . 25 19. Inhibition of protein synthesis by cycloheximide 25 20. Inhibition of Ubiquitination by MG132 25 21. Measurement of nitric oxide 25 …
-
The crystal structure of human Navβ3-Ig domain and its implications
… Na<sub>v</sub>β3-Ig domain, *in vitro*), Proximity Ligation Assay (full-length Na<sub>v</sub>β3, *in vivo*), Atomic Force Microscopy (isolated full-length Na<sub>v</sub>β3, *in vitro*) and Photo-activated Localisation Microscopic experiments (full-length Na<sub>v</sub>β3, *in situ*) …
-
Isopeptide and Ester Bond Ubiquitination Regulate Degradation of the Human Dopamine Receptor 4
… system using immunoprecipitation techniques. A proximity ligation assay was used to quantify isopeptide and ester bond ubiquitination in this protein expression system and to detect ubiquitination of hD4R in mouse primary cortical neurons. Together, these data support the hypothesis that hD4R is …
-
The New Role of Proprotein Convertase Subtilisin/Kexin Type 9: A Connection of Proprotein Convertase Subtilisin/Kexin Type 9, Apolipoprotein B, and Autophagy
… By using 3 different protein–protein interaction assays of co-immunoprecipitation, mammalian two-hybrid system, and <em>in situ</em> proximity ligation assay, we demonstrated a direct protein–protein interaction between PCSK9 and apoB. The impact of this interaction inhibited the physiological …
-
Identification of Previously Unknown Interactions Between G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… GPCRs, we developed a highly multiplexed immunoassay using a suspension bead array (SBA) assay designed to detect RAMP-GPCR complexes. We engineered three epitopetagged RAMPs and 23 epitope-tagged GPCRs, including all members of the secretin-like family of GPCRs, as well as eight other GPCRs. We …
-
Exploring Novel Immunodiagnostics for Prion Disease
… variability and availability by leveraging proximity ligation technology (PLA). We use a repertoire of nine known monoclonal anti-PrP antibodies to assess differences in prion protein conformations and establish “functional” and “non-functional” antibody probe pairs. In light of our results, …
-
Multiplexed Mapping of the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… a multiplexed suspension bead array (SBA) immunoassay to detect GPCR-RAMP complexes from detergent-solubilized cell lysates. To enable multiplexing, we engineered a library of GPCRs and validated a library of anti- GPCR antibodies (Abs). We first prepared a library of dual epitope-tagged GPCR …
-
O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… mutants of vimentin in an array of phenotypic assays, including cell migration, cell cycle progression, and IF dynamics. Here, we show that site-specific modification of the prototypical IF protein vimentin with O-GlcNAc mediates its homotypic protein-protein interactions and is required in …
-
Consequences of stochastic mrna synthesis in a gene regulatory pathway
Gene expression is a stochastic process, with elements of randomness present in both transcription and translation. This stochasticity results in cell-to-cell variation in the amounts of gene products, mRNAs and proteins, and is observed in organisms ranging from bacteria and yeast to higher …
-
ASSESSMENT OF CELL AND METABOLIC IMPACT OF MICROTUBULE TARGETING AGENTS (MTA) IN NEURODEGENERATION AND NEURON MODELS.
… of synuclein oligomers we took advantage of Proximity Ligation Assay technique, and according to our results, OA showed great potential in reducing the oligomeric burden in a neuronal cell model in a dose dependent manner. In addition, OA were proved capable of reducing the toxicity exerted …
-
Functional interactions of overexpressed transcripts associated to Broad Copy Number Aberrations (BCNAs) in solid cancers [Interazioni funzionali di trascritti over-espressi associati ad ampie aberrazioni del numero di copie nei tumori solidi]
… post-trascrizionale. L'immunoistochimica e il Proximity Ligation Assay (PLA) hanno confermato la co-localizzazione di EIF3H e METTL3, supportando il ruolo dei geni Fitness-OverT nella promozione del fenotipo maligno nel CRC. In questa tesi si indaga inoltre l'impatto delle aberrazioni …
-
Molecular Mechanisms of Metabolic Reprogramming in Pancreatic Cancer
… cancer cells. This was confirmed through various assays such as: immunoprecipitation, immunofluorescence, co-capping and proximity ligation assay. The interpretation of inhibitor results envisaged the role of NFҡB pathway during the molecular interactions of MUC13 and Glut-1. To understand the …