Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"proximity dependent biotinylation"”.
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… the targeting and function of TbRP2, I employed proximity-dependent biotin identification (BioID), in combination with quantitative proteomic (SILAC) techniques, to identify putative TbRP2-interacting proteins in vivo. A selected cohort of these proteins were subsequently interrogated by …
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Protein interaction studies on the titin A150 domain using proximity-dependent biotinylation
… molecular mechanism behind HMERF. In this study, proximity dependent biotinylation (PDB) was used to screen for protein interactions. PDB was conducted using the biotin-ligase UltraID fused to a titin fragment. This study encompasses the creation of the titin-UltraID fusion genes, the functional …
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Spatially restricted proteomic mapping of excitatory and inhibitory post-synaptic termini through enzymatic labeling
… called APEX, into the synapse to enable specific proximity dependent biotinylation of the proteomes at the glutamatergic (excitatory) and GABAergic (inhibitory) postsynaptic densities. In doing so, we provide the first selective and comprehensive survey of the inhibitory postsynaptic density, …
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Novel detection and evasion mechanisms pertinent to immunity against Salmonella Typhimurium
… approach for the identification of novel DRPs by proximity-dependent biotinylation using the ascorbate peroxidise enzyme APEX. This approach preferentially labels proteins located at damaged endomembranes for subsequent identification by TMT mass spectrometry. Four enriched proteins CCDC50, …
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Enzyme mediated labeling of synaptic proteins for proteomics and imaging
… the application of peroxidase mediated, proximity-dependent biotinylation to map the proteomes of the excitatory and inhibitory synaptic clefts, which mediate distinctively different intercellular signaling between neurons; the former propagates neuronal signals while the latter inhibits …
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A Role for the HSV Tegument Protein pUL16 in Modulating Cellular Energy Metabolism during HSV-2 Infection
… Consistent with these findings, our proximity-dependent biotinylation experiments identified both mitochondrial-associated membrane and mitochondrial matrix components in proximity to HSV-2 pUL16 during virus infection. While the interaction between pUL16 and host cell mitochondria …