Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"proximity assay"”.
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… with a single stage high throughput screening assay. The work outlined here describes the development of a model system for the study of DNA protein interactions and the development of a high throughput assay for detection of such interactions. A zinc finger protein was designed which will bind …
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Incorporation and analysis of bilayer constituents in nanodiscs
… time, including: scintillation counting, imaging proximity assay, NMR and TLC MALDI-MS. Additionally, the extent and longevity of inclusion of the hydrophobic gadolinium-chelate “C3lipo” doped into the Nanodisc bilayer was analyzed and preliminary cell uptake studies were performed. Finally, the …
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Development of screening assays to test novel integrin antagonists in allergic inflammation
… provides evidence that adopting scintillation proximity assay (SPA) levocabastine (IC50=406 mM), but not the first-generation antihistamine chlorpheniramine, displaces [125I]fibronectin (FN) binding to human a4b1 integrin. This result is supported by flow cytometry analysis, where levocabastine …
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Biochemical Evaluation of SRH Analogs as Potential LuxS Inhibitors
… goal of this project is to establish biochemical assays for the evaluation of small molecules as potential LuxS inhibitors. The first assay is a conventional colorimetric assay that utilizes Ellman’s reagent to quantify the homocysteine byproduct of DPD production by LuxS. For this assay purified …
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Spatial organisation of proto-oncogenes in human haematopoietic progenitor cells
… I have extended and developed a ligation based proximity assay known as enriched 4C. I have coupled this technique with high throughput sequencing to determine genomic regions that spatially co-associate with the proto-oncogenes MLL, ABL1 and BCR. In addition to further developing the laboratory …
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Investigating the physiological function of BACE1 and its role in metabolism
… fed mice were compared alongside a BACE1 proximity assay to determine novel BACE1 interacting proteins. A further study was designed to test the hypothesis that amyloid peptides lead to leptin resistance- a possible contributor to central memory and metabolic impairment. These studies …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
… pathology. Finally, an unbiased protein proximity assay was used to evaluate the SPCA2C interactome and broaden the understanding of SPCA2C function. In HEK293A cells, exogenous SPCA2C expression localized to ER and Golgi compartments and increased resting cytosolic [Ca2+], Ca2+ release …
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Solid-phase combinatorial chemistry and scintillation proximity assays
The Scintillation Proximity Assay (SPA) is a method that is frequently used to detect and quantify the strength of intermolecular interactions between a biological receptor and ligand molecule in aqueous media. This thesis describes the synthesis of scintillant-tagged-compounds for application in a …
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Development of Universal Biosensing Platforms Based on CRISPR/Cas12a systems
… developed method is a plasmonic CRISPR Cas12a assay for colorimetric detection of viral nucleic acid. This assay generates colorimetric signals for nucleic acid amplicons by combining the unique target-induced collateral cleavage activity of Cas12a with plasmon coupling of DNA functionalized …
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The Role of Sleep in Memory Consolidation with an Induced Sleep-Like State in Drosophila AND The Role of Alpha-synuclein in Fatty Acid Metabolism
… free fatty acid, using a scintillation proximity assay (SPA). We characterized the regions of alpha-synuclein protein that are required for oleic acid binding using mutagenesis studies. We over-expressed alpha-synuclein in HEK293FT cells and measured its effect on the rate at which oleic …