Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"protoplast fusion"”.
-
Selection and Characterization of Somatic Cell Hybrids (Protoplast, Fusion)
Fusion of two cell lines with different amino acid analog or herbicide resistances allows selection of heterokaryons from unfused and homologous fusion products through the use of resistance complementation.
-
Protoplast fusion for intraspecific transfer of cytoplasmic male sterility in perennial ryegrass
Contains fulltext : mmubn000001_130152498.pdf (Publisher’s version ) (Open Access)
-
Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
… than 100 anther-derived monoploids. Chemical fusion and electrofusion between pairs selected from 31 superior monoploids resulted in the production of three different groups of intermonoploid somatic hybrids. The hybridity of somatic hybrid plants and calluses was confirmed through PCR-based …
-
Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… due to the effect of the "monoploid sieve" Protoplasts are plant cells, the cell walls of which have been digested by a combination of pectinase and cellulase enzymes. If the protoplasts are cultured in a suitable medium, they can resynthesize the cell walls, divide and regenerate complete …
-
Pure culture and metagenomic approaches to investigate cellulose and xylan degradation
… was subjected to strain improvement through protoplast fusion. Enhanced enzymatic activity was found in one fusant. Differential release of sugars from xylan was observed through gas chromatographic analysis between the parental and fusant cultures. Genome shuffling was observed in 16S rRNA …
-
Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi.
… factors affecting the production and release of protoplasts from the closely related A. fumigatus and A. niger and P. ochrochloron and G. emersonii were investigated. Protoplast yields were found to be greatly dependent upon growth temperature, cultural conditions, mycelial age, mycelial …
-
Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… of sterilities, somatic hybridization via protoplast fusion has been considered an alternative to sexual polyploidization for resynthesizing superior diploids from selected monoploids, and tetraploids from selected diploids and dihaploids. Successful application of somatic hybridization …
-
Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… including anther culture to halve the genome and protoplast fusion to create hybrids between incompatible partners. We applied these techniques to potato to evaluate their potential for breeding highly heterozygous, cross-pollinating species. Four families of monoploids (2n=1x=12), developed from …
-
AFLP Marker Analysis Of Monoploid Potato
Potato haploids have been recent components in protoplast fusion research, strategies to combine wild and cultivated potato germplasm and the generation of economically valuable mutant phenotypes. Additionally, most major genetic mapping and QTL analyses in potato have utilized haploid germplasm to …
-
Transcription Factor Engineering: Tools and Applications
… of gene switches in mammalian cells through a protoplast fusion method, which combines the benefits of simple library creation with screening in a functionally relevant system. Although individual steps of the process were successful, the method proved unsuitable for large scale screening of …