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Showing 1 to 20 of 36 for “"protoplast"”.
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Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… of sterilities, somatic hybridization via protoplast fusion has been considered an alternative to sexual polyploidization for resynthesizing superior diploids from selected monoploids, and tetraploids from selected diploids and dihaploids. Successful application of somatic hybridization …
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Selection and Characterization of Somatic Cell Hybrids (Protoplast, Fusion)
Fusion of two cell lines with different amino acid analog or herbicide resistances allows selection of heterokaryons from unfused and homologous fusion products through the use of resistance complementation.
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Protoplast fusion for intraspecific transfer of cytoplasmic male sterility in perennial ryegrass
Contains fulltext : mmubn000001_130152498.pdf (Publisher’s version ) (Open Access)
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Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
… parental somaclones regenerating from unfused protoplasts (homozygous for one parental band at the loci). One group of somatic hybrids (SH1, SH2 and SH2B) was of particular interest because it resulted from the fusion of a S. phureja monoploid to a high acetylleptinidine-producing monoploid …
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Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… due to the effect of the "monoploid sieve" Protoplasts are plant cells, the cell walls of which have been digested by a combination of pectinase and cellulase enzymes. If the protoplasts are cultured in a suitable medium, they can resynthesize the cell walls, divide and regenerate complete …
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Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them
… for leaf disc regeneration, anther culture, and protoplast culture was tested in the parental, F₁, and F₂ generations of a diploid cultivated primitive potato, S. phureja (2n=2x=24). The parental pair consisted of AM3-8, an anther culture derived homozygous diploid, and NBP2, a heterozygous, …
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Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi.
… factors affecting the production and release of protoplasts from the closely related A. fumigatus and A. niger and P. ochrochloron and G. emersonii were investigated. Protoplast yields were found to be greatly dependent upon growth temperature, cultural conditions, mycelial age, mycelial …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… transformation in both species. Tobacco leaf protoplasts (cv. ”petite Havana”, cv. ”Wisconsin 38”) were used to develop a novel technique – the TAL (thin-alginate-layers) technique. The TAL technique in combination with new culture media resulted in very rapid protoplast development and fast …
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Pure culture and metagenomic approaches to investigate cellulose and xylan degradation
… was subjected to strain improvement through protoplast fusion. Enhanced enzymatic activity was found in one fusant. Differential release of sugars from xylan was observed through gas chromatographic analysis between the parental and fusant cultures. Genome shuffling was observed in 16S rRNA …
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Molecular Studies on Soybean Mosaic Virus-Soybean Interations
… cellular level. For this purpose, an SMV-soybean protoplast system was developed. Protoplast isolation was based on a combined cellulase-pectolyase Y-23 digestion and metrizamide-sorbitol gradient purification protocol. Virus inoculation of protoplasts was facilitated by either polyethelene glycol …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… including anther culture to halve the genome and protoplast fusion to create hybrids between incompatible partners. We applied these techniques to potato to evaluate their potential for breeding highly heterozygous, cross-pollinating species. Four families of monoploids (2n=1x=12), developed from …
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The Use of Morphogenic Transcription Factors to Enhance Plant Regeneration
… transgenic methods of gene editing is the use of protoplasts (cells whose cell wall has been removed) for transient expression and subsequent regeneration of non-GMO, edited plants. However, efficient regeneration of plants from protoplasts is a bottleneck in the implementation of this technique. …
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Characterization of theWound-Induced Signaling Pathway In Tomato
… MAPKs, MPK1/2 and MPK3. Through the use of a protoplast transient expression assay, I have confirmed that MKK2 can phosphorylate MPK1/2 and MPK3, but MKK4 is not expressed in protoplasts. I have shown that negative regulation of MKK4 via conventional protein degradation mechanisms is not …
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Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana
… differentiation is a cellular corpse lacking a protoplast and consisting of a thickened cell wall composed mostly of lignin and cellulose. Little is known about the genetic mechanisms regulating the process of tracheary element differentiation. XCP2 expression localizes to tracheary elements …
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Development of a genetic transformation system in the family Ceratocystidaceae with specific reference to Ceratocystis albifundus
… approach was an attempt to establish a PEG/CaCl2-protoplast transformation protocol in this species. This system can make use of linear DNA, such as PCR products, making it ideal for high-throughput gene editing. The use of a PEG/CaCl2-protoplast transformation system relies on the successful …
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Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
… crops, we devised a strategy involving robust protoplast isolation and the effective application of protoplasts for CRISPR/Cas editing. We offered a detailed, sequential protocol for isolating mesophyll protoplasts from in vitro cultured pennycress and soil-grown camelina. The protocol …
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Decoding the Transcriptional Specificity of Auxin Signaling: A Synthetic Biology Approach
… to process flow cytometry data generated by protoplast transient expression assays. This reproducible workflow automates transformation-based reporter analysis, streamlining quantification of gene expression and effector function in high-throughput experiments. To understand the functional …
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Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
… set in 4x-2x crosses to tetraploid cultivars. Protoplast isolation from in vitro material and extraction of leaf nuclei both in vitro and in vivo were performed on the anther-donor plant, one of its anther-derived monoploids, and a diploid and tetraploid plant derived from callus culture of the …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… transient gene expression were studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the different field strength conditions …
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Characterization of a cold-responsive dehydrin promoter
… of reporter activity after cold stress. The protoplast transformation system, while capable of transient expression of introduced constructs in protoplasts, was difficult for use for cold-inducible expression.
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