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Showing 1 to 20 of 42 for “"proton pumping"”.
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Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase
… c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped …
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Study of the Proton Pumping Mechanism of the Aa3-Type Cytochrome C Oxidase
… followed by stepwise reduction, requiring four protons and four electrons. These four protons are taken up from the negative (N) side of the membrane through two proton conductive channels (D-channel and K-channel). C cO is also a redox-driven proton pump, i.e., the free energy produced by …
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Electrostatic and quantum chemical investigation of the proton pumping mechanism of cytochrome c oxidase
… the free energy of the reduction reaction for proton pumping across the inner-mitochondrial membrane, a process which results in a membrane electrochemical proton gradient. For each oxygen molecule, eight protons are taken up from the matrix of the mitochondria. Four protons together with four …
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Studies on the Structure and Photochemistry of Bacteriorhodopsin (Proton Pumping, Cation Binding, Synthetic Pigments)
Cation Binding By Bacteriorhodopsin (bR). bR normally has a purple color ((lamda)(,max) = 558 nm), hence the name, purple membrane. Atomic absorption spectrophotometry shows that purple membrane binds approximately 4 Ca('++) and Mg('++) per bR at pH = 6.0. Ca('++) and Mg('++) can be removed by …
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Simulation studies of the structure-function relationship of two biological processes: proton pumping in cbb3 oxidase and activation of Parkin
… redox reaction to the active translocation of protons across the membrane, a process that is essential for ATP synthesis. It is mainly present in proteobacteria, and the fact that it is the only terminal oxidase expressed in several clinically relevant human pathogens makes it a potential drug …
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Studies of proton pumping mechanism in BA3 type cytochrome oxidase of thermus thermophilus and CBB3 type cytochrome oxidase of vibrio cholerae
… oxidoreductase superfamily are redox-driven proton pumps which couple the free energy liberated from the reduction of O2 to the translocation of protons across the membrane. The A-family requires two proton input channels (D- and K-channels) to transfer protons used for oxygen reduction …
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The AA3 Type Cytochrome C Oxidase From Rhodobacter Sphaeroides: Insights Into the Proton Pumping Mechanism From the N139D Mutation in the D-Channel
… and generates a transmembrane electrochemical proton gradient. This transmembrane gradient provides the proton-motive force that is used by ATP synthase to generate ATP from ADP and inorganic phosphate. The chemistry of the oxygen reduction reaction involves the uptake of electrons from the N …
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Studies on Critical Proton Pathway Residues in the Aa3-Type Cytochrome C Oxidase From Rhodobacter Sphaeroides
… the free energy of this reaction to translocates protons across the membrane. The oxidase accomplishes oxygen reduction by drawing electron and protons from the opposite sides of the membrane and transfers the pumped protons against the membrane potential through very specific routes within the …
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Studies on critical proton-pathway residues in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides
… the free energy of this reaction to translocates protons across the membrane. The oxidase accomplishes oxygen reduction by drawing electron and protons from the opposite sides of the membrane and transfers the pumped protons against the membrane potential through very specific routes within the …
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Intrinsic uncoupling in the ATP synthase of Escherichia coli. Studies on WT and ε-truncated mutants
… to this monotonic inhibition, however, the proton pumping activity of the enzyme, as estimated under the same conditions by the fluorescence quenching of the Î�pH-sensitive probe ACMA, showed a clearly biphasic progression, both for Pi, increasing from 0 up to approximately 200 µM, and for …
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The Study of Aa3-Type Cytochrome C Oxidase in Rhodobacter Sphaeroides
… water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry reaction of the enzyme is coupled to a proton pump, which substantially contributes to the transmembrane electrochemical gradient. Two proton …
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The study of aa3-type cytochrome c oxidase in Rhodobacter sphaeroides
… water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry reaction of the enzyme is coupled to a proton pump, which substantially contributes to the transmembrane electrochemical gradient. Two proton …
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Cytochrome AA(3) From Rhodobacter Sphaeroides: Affinity Purification and Biophysical Characterization of Site-Directed Mutants
… to rapid kinetic measurements of electron and proton transfers were used to probe the various effects of these mutants on the structure and function of the enzyme. Results were interpreted, when possible, in terms of possible redox-linked proton pumping mechanisms, or alternatively, on a purely …
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Cytochrome AA(3) from Rhodobacter sphaeroides: Affinity purification and biophysical characterization of site-directed mutants
… to rapid kinetic measurements of electron and proton transfers were used to probe the various effects of these mutants on the structure and function of the enzyme. Results were interpreted, when possible, in terms of possible redox-linked proton pumping mechanisms, or alternatively, on a purely …
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The Role of a Neuron-Specific V-ATPase in Synapse Specification, Function, and Maintenance
… (V-) adenosine triphosphatase (ATPase) is a proton-pumping nanomachine consisting of two multi-subunit, reversibly associating protein sectors, the cytosolic V1 sector and the membrane-bound Vo sector. The V1/Vo holoenzyme hydrolyses ATP to translocate protons across biological membranes …
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Investigating the rate-limiting step of mitochondrial complex I catalysis
… (Q) reduction to the translocation of four protons across the inner mitochondrial membrane, contributing to the proton motive force (PMF) used to synthesise ATP. Although structural knowledge of complex I is now extensive, the mechanism by which it couples the redox energy for proton …
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Structure and Function Relationships in Cytochrome Bo(3) Oxidase and Cytochrome Bd-I Oxidase From Escherichia Coli
… the Peroxy species. Support for a cytochrome bo3 proton pumping mechanism is presented in which a histidine ligand for CuB dissociates upon reduction. The other terminal oxidase, cytochrome bd-I oxidase, is not related to the heme/copper superfamily and was studied through sequence analyses and …
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An updated genome scale metabolic reconstruction of methanosarcina barkeri
… unclear metabolic pathways. For methanogenesis, proton pumping has been updated to reflect an improved knowledge of the pathway. Cofactor biosynthesis has been updated to reflect a better understanding of methanogen anabolism. Metabolic networks such as pentose phosphate pathway, amino acid …
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Exploring the effects of proteorhodopsin on the physiology of native and heterologous hosts
… yields presumably as a result of light-driven proton pumping by PR. This work illustrates that PR is part of orchestrated response to light in a PR-containing isolate, but can also influence the growth of a heterologous host on its own. Further refinement of the genetic background of E. coli …
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Lst1p and Exp1p act in parallel pathways to export the plasma membrane H⁺-ATPase from the ER in S. cerevisiae
… growth on acidic medium (pH 3.0) and decreased proton pumping activity. Pma1p accumulates in the ER of Ist1A strains, while other cargo molecules such as invertase and CPY are transported with wildtype kinetics. Like Sec24p, Lst1p specifically binds the COPII component Sec23p. Thus, we propose …
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