Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"proton channel"”.
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Functional and Structural Studies of the Human Voltage-Gated Proton Channel
<p>The activity of voltage-gated cation channels underlies the action potentials that allow for neuronal signaling and muscle contraction. The canonical family of voltage-gated K<sup>+</sup>, Na<sup>+</sup> and Ca<sup>2+</sup> channels has been the subject of extensive electrophysiological, …
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The HV1 proton channel of Lingulodinium polyedrum localizes to the bioluminescent scintillon
… and colleagues predicted the existence of a proton selective channel that opens in response to depolarizing voltage (H<sub>V</sub>1) across the vacuole membrane of bioluminescent dinoflagellates and conducts protons into specialized luminescence compartments (scintillons), thus causing the pH …
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Structure-Function Relationship Studies of the Rhodobacter Sphaeroides Cytochrome AA(3)
… by this reaction to generate an electrochemical proton gradient. This proton gradient can be used to produce ATP by ATP-Synthase. Rhodobacter sphaeroides cytochrome c oxidase, the focus of this thesis, serves as an excellent model to systematically understand the more complicated mammalian …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
The voltage-gated proton channel Hv1 is crucial for maintaining cellular pH homeostasis by regulating proton movement across membranes, playing a vital role in acid extrusion and intracellular pH regulation. Dysregulation of Hv1 is associated with various diseases, including cancer, where it helps …
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The Heme -Copper Oxidoreductase Superfamily: Genomics and Structural Analyses
… cofactor in the A-, B-, and C-families of proton pumping respiratory oxidases, demonstrating that these enzymes likely share a common reaction mechanism. Finally we predict the properties of proton channels within the C-family. Modeling and sequence analysis suggests that the C-family has …
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Current understanding on cytochrome bd quinol oxidase of Escherichia coli a mutagenesis, kinetics and spectroscopic study
… data further suggested a series of protonatable groups forming a proton channel located in the membrane to facilitate the proton translocation from cytoplasm to the heme b595 / heme d binuclear center. With help of the increasing database of available cytochrome bd oxidase sequences, …
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Structure and dynamics of influenza M2 proton channels from solid-state NMR
… (M2) is an essential viral protein that conducts protons in the endosomes of infected host cells and induces membrane curvature to facilitate virus budding. M2's proton channel activity is encapsulated by a transmembrane domain (TM) that is targeted by the FDA-approved antiviral drugs amantadine …
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Inheritance, innovation, and adaptation of immune systems: Regulation of the Innate Immunity Sensor Stimulator of Interferon Genes (STING)
… transmembrane domain of human STING forms a proton channel, which plays a role in inducing non-canonical autophagy and inflammasome activation. Additionally, we demonstrated that mammalian cells employ DNAJC13 to prevent the accumulation of aggregated STING following its activation. This …
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Functional Genomic and Image-Based Screening Approaches for Probing Host-Pathogen Interactions
… of a novel function of the STING protein as a proton channel.
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Conformation of Transmembrane Segments of a Protein by a Coarse Grain Model
<p>The human voltage-gated proton channels (hH<sub>V</sub>1) are critical in many physiological functions and control proton conduction in the cell. This process is governed by the cooperative response of different transmembrane segments of the protein. It is believed that the two subunits of the …
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Solid-state nuclear magnetic resonance investigations of the influenza M2 protein : structure and dynamics characterization of channel gating and conduction
… are investigated in the influenza AM2 and BM2 proton channels bound to native-like lipid bilayers. Using pH-dependent 15N chemical shifts of the conserved Histidine residue, we have found that the proton conduction mechanism is the same in all of the M2 channels (WT AM2, S3 IN AM2, BM2) …
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The Role Of The Membrane Proximal Region Of The M2 Cytoplasmic Tail In Virus Replication
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. The primary aim of this thesis was to investigate the role of the membrane proximal region, residues 46-69, of the M2 cytoplasmic tail during virus replication. A cholesterol …
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I) Hv1, LBP and LCF reconstitution in Liposomes II) Signed, Sealed, and Delivered: CPP Technology Delivers Active Protein Cargo
… Protein (LBP), Luciferase (LCF), voltage-gated proton channel (Hv1), as well as the substrate Luciferin (LH<sub>2</sub>). Bioluminescence is triggered by an internal drop in pH of the scintillon initiated by the voltage activation of Hv1 in the membrane. The first focus of this study is to use a …
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Mutagenesis and Spectroscopic Studies on Cytochrome Bd Quinol Oxidase of Escherichia Coli
… to the periplasmic side of the membrane. A proton channel now becomes evident so that protons can access the binuclear center and the water resulting from oxygen reduction can be released. Spectroscopic studies of wild type and mutant enzymes using NMR and FTIR were also performed. FTIR …
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Studies of Cytochrome C Oxidases From Rhodobacter Sphaeroides and Vibrio Cholerae
… the reducing oxygen to water and pumping the protons across the membrane. The second largest group, C-type, is also known as cbb3 cytochrome c oxidase which is almost exclusively found in the Proteobacteria. All the current studies show that cytochrome cbb3 oxidases apply quite distinct …
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Studies of cytochrome c oxidases from Rhodobacter sphaeroides and Vibrio cholerae
… the reducing oxygen to water and pumping the protons across the membrane. The second largest group, C-type, is also known as cbb3 cytochrome c oxidase which is almost exclusively found in the Proteobacteria. All the current studies show that cytochrome cbb3 oxidases apply quite distinct …
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Structural and biochemical studies of the regulation and catalytic mechanism of ATP synthase
… of ATP from ADP and orthophosphate by using the proton motive force established across a membrane by photosynthesis or oxidative phosphorylation. The ATP synthase of eukaryotic mitochondria is located in the inner membrane and is comprised of two domains. The globular F1 domain protrudes into the …
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Biochemical characterization of a-type heme-copper oxidases in escherichia coli, bacillus subtilis and thermus thermophilus
… free energy of oxygen reduction and translocate protons across membrane to generate a proton electrochemical gradient, which was used to produce ATP by ATP synthase. Based on the sequences and structures of core subunits, they are classified into 3 types. A-type oxidases are by far the most …
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Biochemical characterization of C-family heme copper oxygen reductase
… c on the P-side of the membrane, and four protons from the cytosol on the N-side, to molecular O2 bound at the active site that results in the production of water. The excess energy released in this reaction is used to pump protons from the N-side to the P-side. These processes establish an …
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The little engine that could: Characterization of noncanonical components in the speed-variable flagellar motor of the symbiotic soil bacterium Sinorhizobium meliloti
The bacterial flagellum is a fascinating corkscrew-shaped macromolecular rotary machine used primarily to propel bacterial cells through their environment via the conversion of chemical potential energy into rotational power and thrust. Flagella are the principal targets of complex chemotaxis …