Global ETD Search
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Showing 1 to 12 of 12 for “"protofilaments"”.
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The Conformational Dynamics of the Polymorphic Amyloid β Protofilaments
… the interaction between amyloid β (Aβ) protofilaments and model bilayers.|The community, as a whole, does not know the 3D structure of the Aβ protofilament. Therefore, we studied two polymorphic Aβ (9-40) structures [1] as likely conformations. We relaxed the structures through normal …
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Structure-function analysis of ZapC, an FtsZ-ring stabilizer, in Escherichia coli cytokinesis
… by increasing lateral interactions amongst FtsZ protofilaments in the Z-ring. ZapA-D are recruited during early cytokinesis and have overlapping functions in stabilizing FtsZ at midcell, but share no primary sequence identity. The focus of this study is on ZapC, a small cytoplasmic protein that …
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pH MODULATION OF FIBRIL DISSOCIATION AND COPPER BINDING PROPERTIES OF THE PRION PROTEIN
… dissociate both laterally and axially to form protofilaments. About 5% of the protofilaments are short enough to be considered soluble and contain ~100-300 monomers per structure; these also retain the biophysical characteristics of the filaments. We propose that protonation of His residues and …
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pH MODULATION OF FIBRIL DISSOCIATION AND COPPER BINDING PROPERTIES OF THE PRION PROTEIN
… dissociate both laterally and axially to form protofilaments. About 5% of the protofilaments are short enough to be considered soluble and contain ~100-300 monomers per structure; these also retain the biophysical characteristics of the filaments. We propose that protonation of His residues and …
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Regulation of Tubulin dynamics by the +Tip tracking protein Mal3
… probably stabilizes the MT structure by bridging protofilaments together. Deletion mutant analysis suggests that contact with one of the protofilaments is via an interaction between the charged tails of tubulin and Mal3. Mal3 MT binding domain structure was solved by X-ray crystallography so that …
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Prosthecobacter BtubAB form bacterial mini microtubules
… it probably functions as single homopolymeric protofilaments, possibly through treadmilling dynamics. There is strong divergence of the biological functions in the tubulin/FtsZ superfamily. Microtubules are involved in fundamental processes such as motility, transport and chromosomal …
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Biochemical and Structural Studies of Amyloid Proteins
… potent at inducing Ca2+ influx, whereas longer protofilaments are the most potent inducers of TNFα production. My results suggest that the mechanism by which aggregates damage cells changes as aggregation proceeds, as longer aggregates with different structures are formed. Protofilaments with a …
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Molecular mechanisms of kinetochore microtubule attachment
… Skal complex directly interacts with the peeling protofilaments present at the depolymerizing microtubule end, suggesting a unique mechanism by which the Skal complex remains attached to depolymerizing microtubules. Finally, we demonstrate that the Skal microtubule-binding domain has two conserved …
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FtsZ Protofilament Curvature is the Opposite of Tubulin Rings
… tubulin homolog FtsZ assembles straight protofilaments (pfs) that form the scaffold of the cytokinetic Z ring. These pfs can adopt a curved conformation forming a miniring or spiral tube 24 nm in diameter. Tubulin pfs also have a curved conformation, forming 42 nm tubulin rings. We have …
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Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… form of FtsA revealed that FtsA forms actin-like protofilaments with a repeat of 48 Å. An identical repeat length was observed when FtsA was polymerised using a lipid monolayer surface and FtsAs from three organisms formed polymers in cells when overexpressed, as observed by electron …
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Molecular Mechanism By Which The Escherichia Coli Nucleoid Occlusion Factor, Slma, Keeps Cytokinesis In Check
… FtsZ protein. FtsZ self-assembles into linear protofilaments in a GTP dependent manner to form a cytoskeletal scaffold called the Z-ring. The Z-ring provides the framework for the assembly of the division apparatus and determines the site of cytokinesis. The total amount of FtsZ molecules in a …
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EFFECT OF MICROTUBULE-TARGETING AGENTS ON STRUCTURAL SIGNALING AND CHEMICAL TRANSPORT IN CELLS
… polymerize in a head-to-tail fashion to create protofilaments, which subsequently assemble into hollow cylindrical polar filaments. Microtubules’ behavior and function depend on their polymerization dynamics, with the first layer of regulation being the GTPase cycle of tubulin and its addition …