Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"proteomics approach"”.
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Biomarker Discovery in Autoimmune Diseases: A Proteomics Approach
… marker discovery. Mass spectrometry-based proteomics biomarker discovery holds the ability to interrogate a constellation of proteins simultaneously in a high-throughput manner to uncover a panel of markers that are specific to the presence of a disease. However, the rate of introduction of …
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A Proteomics Approach to Investigate Uropathogenic Escherichia coli
… with a focus on ST127, using a quantitative proteomics approach. We employed a classical shotgun proteomics approach to analyse proteins extracted from multiple strains of UPEC following growth in various environments (Lysogeny broth, artificial urine medium and co-culture with uroepithelial …
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Redefining endothelial progenitor cells using a proteomics approach
… the advantage of using an unbiased proteomic approach to assess cellular phenotypes and advise caution in attributing the benefits inclinical trials using unselected bone marrow mononuclear cells (BMCs) to stem cell-mediated repair.
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The Proteomics Approach To Evolutionary Computation: An Analysis Of Pr
… so does the complexity of the problems that we approach using evolutionary search techniques. There are recent approaches to deal with the problem of scaling evolutionary methods to cope with highly complex difficult problems. Many of these approaches are biologically inspired and share an …
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A Proteomics Approach to the Examination of Proteins in Marine Systems
… essential. This dissertation utilizes a combined approach of molecular biomarkers and proteomic investigations to elucidate historic source material contributions and microbial protein production to contribute to a more thorough understanding of the marine carbon and nitrogen cycles. The …
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Proteomics approach to investigate separation behavior of proteins in ion-exchange chromatography
… depended on the base matrix type. This approach could be extended to host protein extracts to provide guidance for purification of recombinant proteins or choice of a suitable host for a particular recombinant protein.</p>
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Characterisation of novel and complex mechanisms of antobiotic resistance using a proteomics approach
… work also emphasised the advantages of using proteomics for profiling the expression of resistance proteins, including the detection of specific enzymes, such as CTX-M ESBLs.
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Understanding adaptive regulation of skeletal muscle physiology in the Weddell seal: a proteomics approach
… the use of cross-species analysis and develop a proteomics protocol for use in diving mammals. These steps are necessary in order to ultimately use proteomics to identify age class protein signatures and better understand the molecular regulation of the physiological changes that couple the …
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Study of the cell cycle interactome of Arabidopsis thaliana through a targeted proteomics approach
… of the thesis, an overview is given on proteomic approaches with an emphasis towards study of protein-protein interactions and towards plant proteomic sudies. Next, we describe the development and application of a high-througput tandem affinity purification (TAP)/mass spectrometry platform for …
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A hair bundle proteomics approach to discovering actin regulatory proteins in inner ear stereocilia
… in the hearing system, I decided to pursue a proteomics-based approach to discover proteins that play a role in stereocilia function. I employed a modified "twist-off" technique to isolate hair bundle proteins, and I developed a method to purify proteins and to process them for analysis using …
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An Activity-Based Proteomics Approach for Identifying Ajoene's S-thiolation Protein Targets in Blood and Cancer Cells
… of cellular proteins. Chapter Five constitutes a proteomics study into ajoene's primary protein targets in the MDA-MB-231 cell line using our biotin-ajoene probe. Streptavidin-coated magnetic beads in conjunction with an affinity purification mass spectrometry protocol allowed the isolation and …
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Characterizing blood plasma proteins from organ tissue in rainbow trout (Oncorhynchus mykiss) using a non-targeted proteomics approach
Protein expression patterns adapt to various cues to meet the needs of an organism. The dynamicity of an organism’s proteome can therefore reveal information about an organism’s health. Proteome databases contain limited information regarding organisms outside of medicinal biology. The Uniprot …
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Proteomics approach on the identification of virulence factors of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) and further characterization of two effectors: EspB and NleI
… to have two dominant forms in extracellular proteomics profiles of EPEC and EHEC. EspB was shown to be translocated into the host cells by TTSSs and was indicated to be a TTSS encoded effector. Furthermore, a novel effector, NleI, was identified from EPEC sepL and sepD mutants by comparative …
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Identification of Novel Fluid Biomarkers for Alzheimer's Disease
… the cerebrospinal fluid: CSF) using an unbiased proteomics approach: two-dimensional difference gel electrophoresis with liquid chromatography tandem mass spectrometry). From this, we identified 47 proteins that differed in abundance between cognitively normal: Clinical Dementia Rating [CDR] 0) …
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Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
… and to identify DCUN1D1 inhibitors using a proteomics approach. Immunoprecipitation-coupled mass spectrometry was performed to identify DCUN1D1 binding partners and we identified some known substrates of DCUN1D1 in the form of cullin 3, cullin 4B and cullin 5. We also observed that the …
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Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
… and to identify DCUN1D1 inhibitors using a proteomics approach. Immunoprecipitation-coupled mass spectrometry was performed to identify DCUN1D1 binding partners and we identified some known substrates of DCUN1D1 in the form of cullin 3, cullin 4B and cullin 5. We also observed that the …
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ECLIPSED DUAL DISTRIBUTION OF MITOCHONDRIAL PROTEINS IN EUKARYOTES: UBIQUITINATION IN YEAST MITOCHONDRIA AS A PARADIGM
… machinery. Additionally, the iTRAQ proteomics approach applied in the current thesis sets the ground for systematic screening of dual targeting regulation. We believe that the present studies will have an impact on discovery of new molecular events by which dual targeting, especially …
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THE BIO-MOLECULAR ANALYSIS OF TITANIUM DIOXIDE NANOPARTICLES EXPOSURE ON CARDIOMYOCYTES
… relevant safety biological endpoints to work. To approach this bottleneck, the use of quantitative proteomics approach using isobaric tags for relative and absolute quantitation (iTRAQ™) was proposed to identify these biological endpoints. Using the H9c2 cardiomyocyte as an <i>in vitro</i> model, …
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The role of PLK1 in Hepatitis B Virus (HBV) infection of the liver
… by loss of function as well as gain of function approaches that PLK1 inhibition suppresses viral replication both in vitro and in vivo. I also show that HBc is a phosphorylation substrate for PLK1 in vitro and I mapped the PLK1 phosphorylation sites to HBc residues S168, S176 and S178. Finally, I …
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Acute Nitrate Exposure Causes Proteomic Changes Consistent with the Regulation of Reactive Oxygen and Nitrogen Species
… changes in protein expression, using a gel-based proteomics approach. Of the 642 protein spots detected, we found that 24 proteins (15 identified) changed expression in response to a 6-hour exposure to nitrate concentrations ranging from 0-73 mg/L, values that characterize highly contaminated …
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