Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"proteomic methods"”.
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Enhanced Proteomic Methods to Decipher How the Novel Protein SNED1 Modulates the Extracellular Matrix
… my mentor, Dr. Alexandra Naba developed a proteomic pipeline tailored to study the protein composition of the ECM, which has since become the method of choice in studying the ECM. Using this method, Dr. Naba identified a novel ECM protein, SNED1, as being only present in the ECM of highly …
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Characterization of the White-rot Fungus, Phanerochaete carnosa, through Proteomic Methods and Compositional Analysis of Decayed Wood FibreCharacterization of the White-rot Fungus, Phanerochaete carnosa, through Proteomic Methods and Compositional Analysis of Decayed Wood Fibre
… of the corresponding secretome using proteomic techniques. Through this study, cultivation methods for P. carnosa were established and biochemical assays for protein activity and quantification were developed. Analytical methods, including FTIR and ToF-SIMS were used to characterize …
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Proteomic Approaches to Study the Mode of Action of Safeners for Chloroacetamide Herbicides in Triticum Tauschii
… tissues of Triticum tauschii seedlings with proteomic methods. These proteins were classified into 3 major categories based on protein expression patterns and further categorized into functional groups. The largest functional category of safener-responsive proteins belongs to xenobiotic …
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Investigation of hypothalamic molecular mechanisms underlying narcolepsy and obesity through DNMT1 and leptin receptor signalling
… mutant mice. The second project used proximity proteomic methods to identify proteins that regulate human leptin receptor (LEPR) signalling, since leptin signalling is essential for normal body weight regulation. Several candidate proteins were identified that may negatively regulate signalling …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… of propionibacteria and gives an overview of proteomic methods focusing on 2-DE studies related to probiotic bacteria and P. freudenreichii. The aim of the experimental part was to use 2-DE to obtain proteome maps of two strains of P. freudenreichii and detect inter-strain differential protein …
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Biochemical and molecular basis for metabolism-based atrazine resistance in Amaranthus tuberculatus
… protein purification techniques combined with proteomic methods to further investigate the hypothesis that enhanced metabolic detoxification of atrazine, catalyzed by distinct GST isozymes, may confer resistance in ACR and MCR. ACR and MCR showed higher GST specific activities towards atrazine …
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Modulation of protein expression in Mycobacterium tuberculosis-stimulated TB- IRIS patient-derived PBMCs by omega n-3 polyunsaturated fatty acids
… hypothesis, we used mass spectrometry (MS)-based proteomic methods to probe the secretome of peripheral blood mononuclear cells (PBMCs) re-stimulated ex vivo with Mycobacterium tuberculosis (Mtb) whole cell lysate (WCL), and treated with n-3 PUFA. Optimization experiments were performed on cells …
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Human antibody-mediated immunity against infection and parasite fecundity in Schistosoma mansoni and Schistosoma haematobium infection
Through a combination of epidemiological and proteomic methods, the aim of this thesis was to advance understanding of antibody-driven immune responses that partially protect against infection and reduce fecundity in human schistosome infections. The relative contribution of exposure to infection …
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Comparative proteomic analysis of Australian epidemic Bordetella pertussis
… of the host’s immunisation background. Proteomic methods for secretome and surfaceome analysis was established. The B. pertussis secretome was characterised in Stainer-Scholte (SS) and Thalen-IJessel (THIJS) media. Ten virulence proteins were detected uniquely in THIJS while an …
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Venom Yield, Regeneration, and Composition in the Centipede Scolopendra Polymorpha
… composition of <em>S. polymorpha </em>using proteomic methods. I demonstrated that the venom of <em>S. polymorpha </em>is complex, generating 23 bands by SDS-PAGE and 56 peaks by RP-FPLC. MALDI TOF MS revealed hundreds of components with masses ranging from 1014.5 to 82863.9 Da. The …
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Detektion relevanter Moleküle zur Diagnostik des Harnblasenkarzinoms mittels MALDI imaging
… technique of MALDI imaging. Compared to other proteomic tools MALDI imaging is a potential new technique providing higher sensitivity and specificity comparing and classifying fresh frozen tissue biopsies of bladder cancer. Using MALDI imaging different protein patterns could be identified, …
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A DE NOVO COMPUTATIONAL DISCOVERY PLATFORM FROM RNA TO PROTEIN
… efforts. In this regard, transcriptomic and proteomic methods can help elucidate transcript functions and the detection of actively translated Open Reading Frames (ORFs). The main goal of this project is the development of methods for de novo identifications of RNAs, ORFs, and proteins …
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Molecular and physiological investigation of trace metal stress in seagrass, Zostera muelleri
… toxicity responses at transcriptomic and proteomic levels, it also provides candidate molecular markers for future toxicology studies and seagrass monitoring. This PhD thesis also evaluates a protein-centric and four peptide-centric proteomic methods and proposed an optimised peptide …
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The identification of prostate cancer associated tumour antigens and biomarkers
… separation of tumour proteins by conventional proteomic methods (2-DE and mass spectrometry) with serological screening using serum antibodies, to identify immunogenic proteins in cancer. This project aims to identify TAAs and/or biomarkers for PCa which on subsequent validation, can be …
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Characterization of the major extrapallial fluid protein of the mollusc, Mytilus edulis: Implications for function
… of the EP protein by RT-PCR and cDNA sequencing methods. The EP protein is comprised of 213 amino acids post cleavage of a signal peptide of 23 amino acids, and is rich in His, Glu and Asp residues. The consensus site of N-glycosylation "NHTE" and the intramolecular disulfide bond of the protein …
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Transcriptomic and proteomic analysis of anhydrobiosis in Panagrolaimus superbus and Caenorhabditis elegans dauer larvae
… C. elegans dauer larvae by using quantitative proteomic methods. In C. elegans dauer larvae and P. superbus, the following processes and pathways were implicated in the response to desiccation at both the transcriptome and proteome level: molecular chaperone activity, molecular shield activity, …
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The Effect of Antibiotics on Toxin Gene Expression in PVL-positive Staphylococcus aureus Strains
… using quantitative PCR and protein levels using proteomic methods. Western blotting was used to study differential expression of Spa at protein levels. Data showed that the 10 clinical isolates belong to seven clonal complexes (CCs), which are CC1, CC5, CC8, CC22, CC30, CC88, and CC121. Genetic …
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… In this thesis we used two orthologous proteomic methods to identify novel insulin-responsive protein translocation events in adipocytes, with the premise that protein localisation is intricately linked to protein function. By identifying new insulin-regulated protein translocation …
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Ocean acidification influences on physiology and epigenetics in the Pacific oyster (Crassostrea gigas)
… to withstand more stressful conditions. Genomic methods can elucidate molecular mechanisms that contribute to phenotypic plasticity, allowing for a deeper understanding of how physiological processes will be impacted by low pH. My dissertation examines the effects of ocean acidification on the …
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Human Cytomegalovirus and the Neutrophil
… here in a primary cell type, allows quantitative proteomics to be performed for the first time in the neutrophil. This work demonstrates that the technique provides a comprehensive readout of all neutrophil plasma membrane proteins in a sample, with high plasma membrane purity and minimal …
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