Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"proteome analysis"”.
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Investigation of ACE-overexpression in Myeloid Cell Lines through Whole-Proteome Analysis
… own murine MS results. Murine phosphoproteomic analysis identified ERK2/MAPK1 and PKA kinase-substrate enrichment with ACE overexpression. Using the human cell line, THP-1, Lis-Trp uptake was quantified through MS and ACE enzymatic activity assays over two hours. Minimal internalization took …
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Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.
… production of the antimalarial artemisinin: a proteome analysis, a quantitative cDNA amplified fragment length polymorphism analysis (cDNA AFLP) and the construction of full length Expressed Sequence Tag (EST) cDNA libraries. We identified proteins that were differentially expressed between …
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Differential proteome analysis of human lung epithelial cells following exposure to aromatic volatile organic compounds
… reported, effects on complex protein networks (proteome) have not been investigated. However, this information is indispensable when trying to ascertain a mechanism for VOC action on the cellular level and establishing preventive strategies. For this study, the alveolar epithelial cell line A549 …
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Clarification of the xanthan biosynthesis mechanism in Xanthomonas campestris pv. campestris B100 by mass spectrometry-based proteome analysis
… D-mannose.<br /><br /> In the first project, the proteome profiling of Xanthomonas campestris pv. campestris over the time of cultivation was considered. The consumption of nutrition and the amount of synthesized xanthan were particularly interesting. The reference database indicates that 2416 …
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… development leading to the first high-throughput proteome analysis of Methanosarcina acetivorans utilizing a custom FT mass spectrometer. It outlines a two dimensional separations platform that utilizes a 96 well plate nanospray robot to facilitate automated analysis on the mass spectrometer. This …
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PKCε and cardioprotection : an exploration of putative mechanisms
… explain the cardioprotective role of PKCε. Sub-proteome analysis has identified interactions between activated PKCε and various mitochondrial proteins, which orchestrate mitochondrial homeostasis, including proteins governing mitochondrial oxidative phosphorylation, electron transfer, ion …
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<i>Haemophilus influenzae</i> modulins in chronic obstructive pulmonary disease airway inflammation
… were enriched from culture supernatant fluid. Proteome analysis of the enriched fractions revealed 27 candidate proteins. Further analysis of four proteins, ClpB, OmpP2, TonB, and RelA, suggested a potential role in the IL-8 response. A fifth protein, FtsH, showed no such response. Study of the …
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BRIDGE : a bioinformatics software platform for the integration of heterogeneous data from genomic explorations
… genome annotation, EMMA for transcriptome data analysis, ProDB for proteome analysis and GOPArc as a Gene Ontology and pathway architecture). A project management system was established for combining different data sources. In addition to an application programmer's interface (API) all …
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Proteomic analysis of Pseudomonas putida F1 during growth on toluene-phenol mixtures
… a proteomics approach was utilized. An optimal proteome analysis procedure was developed and the proteomes of Pseudomonas putida F1 grown on either a single substrate (toluene or phenol) or a toluene-phenol mixture were analyzed by two-dimensional polyacrylamide gel electrophoresis. Three groups …
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Effects of Lubabegron supplementation on carcass traits, muscle fiber type, proteome profile and meat quality attributes of finished feedlot steers
… purpose of objective color, muscle fiber typing, proteome analysis, and the evaluation of the effect of postmortem aging on tenderness and palatability. As dose increased (P < 0.05) to 3.2 and 5.0 g/ton steaks became less (P < 0.05) red (a*), less (P < 0.05) yellow (b*), and less (P < 0.05) …
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Metal-protein interactions for proteomics applications
… a vital component. However the complexity of the proteome poses an immense challenge for analytical chemists to explore it comprehensively. Currently mass spectrometry is at the forefront of proteome analysis, providing great capabilities to couple with modern separation techniques for wide …
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Microfluidic Interfaces for Mass Spectrometry: Methods and Applications
… has been an unprecedented growth of biomolecule analysis using mass spectrometry (MS). One of the most popular applications for mass spectrometry is the field of proteomics, which has emerged as the next scientific challenge in the post-genome era. One critical step in proteomic analysis is …
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Use of an Animal Model of Group A Streptococcal Infection to Identify Factors Important for Virulence
… of speB to wild type levels. A microarray analysis of the spt10R/bgaA mutant revealed significant transcriptional changes in genes involved in virulence and carbohydrate metabolism. In addition, spt10SR and bgaA were found to be part of a four-gene operon that is repressed by the CovR …
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Nuclear proteomics and transcription factor profiling in Chlamydomonas reinhardtii
… for isolation of nuclei and performed nuclear proteome analysis using shotgun proteomics. This analysis permitted us to improve the genome annotation previously published and to discover conserved and enriched protein motifs among the nuclear proteins. In another approach, a quantitative RT-PCR …
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The Contribution of Retrotransposons to the Transcriptomes of Murine Somatic Cells
… identity. Using differential expression and proteome analysis, I present evidence for post-transcriptional regulation of parent transcripts by retrocopy RNA, possibly through stabilisation of the parent RNA. I also find that while retrocopy expression is not necessarily shared between cell …
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Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression
… Here, results are presented from a genetic analysis of 15 naturally occurring S. coronatus populations. Analysis of molecular variance (AMOVA) and phylogenetic analysis (based on non-coding nuclear and plastid markers) suggest that Ni accumulation may have evolved twice within S. coronatus, …
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The in vitro effects of heavy metals and nanoparticles on the immune system
… cytokines of the acquired immune system and a proteome profile analysis. The first objective of this study was to assess the effects Cd, Ag and Cu on immune system biomarkers. No effects on were seen on cultures not stimulated by LPS. Cd was more cytotoxic than Ag, with Cu having no effect on …
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