Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"proteoliposomes"”.
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Reconstituting APP and BACE in proteoliposomes to characterize lipid requirements for β-secretase activity
… to quantitatively follow the beta-cleavage in proteoliposomes, and we have shown that the rate of cleavage in total brain lipid proteoliposomes is higher than in phosphatidylcholine vesicles. We have also studied partitioning of APP and BACE in GUVs between liquid ordered (lo) and liquid …
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HIV-1 Immune Escape and Neutralizing Antibodies : Solid Phase Proteoliposomes Containing HIV-1 Envelope Glycoproteins as Antigens and Immunogens and HIV-1 Core Envelope Glycoproteins Deficient in T-Helper Epitopes
… remains a major challenge. Here, solid-phase proteoliposomes containing native, trimeric HIV-1 envelope glycoprotein complexes that mimic the trimeric complex as it is found on the viral surface have been designed. In a comparative immunogenicity study, these proteoliposomes were shown to …
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Investigations into the Lipid Transport Mechanisms and Energetics of Bacterial ATP-Binding Cassette (ABC) Transporters MsbA and LmrA
… ethidium efflux, and when directed outwardly in proteoliposomes gives rise to ethidium uptake by purified inside-out oriented LmrA. However, a detailed understanding of the sodium coupling mechanisms of LmrA is limited. LmrA has also been shown to mediate the transport of phospholipids, but the …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… liposomes and carefully characterized the proteoliposomes containing v- and t-SNAREs. Fusion between the v- and t-SNARE proteoliposomes was then monitored with a lipid mixing assay. Interestingly, little fusion was observed. The results suggest that the SNAREs alone are not sufficient to …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were generated by web-based and in-house programs. The computational analysis implies that VGLUT1 protein appears to have 12-transmembrane domains. Chemical and enzymatic …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were generated by web-based and in-house programs. The computational analysis implies that VGLUT1 protein appears to have 12-transmembrane domains. Chemical and enzymatic …
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Molecular and Biochemical Characterisation of SiaP as a Sialic Acid Binding Protein Component of a TRAP Transporter of Sialic Acid.
… SiaPQM in vitro, using SiaQM reconstituted into proteoliposomes, and in vivo, using expression of siaPQM in an E. coli strain lacking its native sialic acid transporter, NanT. It is demonstrated that stabilisation of the carboxylate group of sialic acid by the totally conserved Arginine-147 is …
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Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1
… (STC)" or" cytochrome" c." Using" these" proteoliposomes" the" rate" of" electron"transfer"across"MtrCAB"was"investigated."MtrCAB"was"seen"to"enable"the"reduction" and"oxidation"of"methyl"viologen"and"STC,"but"only"the"reduction"of"cytochrome"c.""STC"was" reduced" during" the" …
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Deciphering Multidrug Efflux: Substrate Interactions and Transport Mechanisms in PatAB and NorM-VC
… solution or when reconstituted into nanodiscs or proteoliposomes, similar to previous reports for Pdr5. While this presents challenges for functional studies, the successful reconstitution of PatAB into peptidiscs establishes a foundation for future structural studies, potentially providing new …
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Regulation of the mTORC1 growth pathway by amino acids
… on Rag activity. SLC38A9 reconstituted in proteoliposomes transports arginine, an abundant amino acid in the lysosome and necessary for mTORC1 pathway activity. These results place SLC38A9 between amino acids and the Rag GTPases and are consistent with the notion that amino acids are sensed …
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Sample preparation of membrane proteins suitable for solid-state MAS NMR and development of assignment strategies
… to be studied by solid-state MAS NMR. Proteoliposomes, poorly diffracting 2D crystals and a PEG precipitate of the outer membrane protein G (OmpG) were prepared as a model system for large MPs. Results from this work, combined with data found in the literature, show that highly …
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Investigating the function of the multidrug transporter ABCG2
… as another novel substrate. Experiments with proteoliposomes containing purified inside-out oriented ABCG2 revealed that an interior alkaline ΔpH was necessary and sufficient for ABCG2-mediated ethidium transport. These results are relevant physiologically in the gut where, in acidic regions, …
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Regulation of amino acid transport across the lysosomal surface by the mTORC1 pathway
… bind SLC38A9, promote its transport activity in proteoliposomes reconstituted with wild-type SLC38A9. We believe the purpose of this regulation is to efflux amino acids from the lysosome during starvation. Overall, we identified the mechanism by which mTORC1 regulates the efflux of essential …
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Structural and Functional Studies of Munc18 and SNARE Proteins
… into separate liposomes. Fusion between these proteoliposomes in the presence of Munc18-1 was monitored with a lipid mixing assay. Intriguingly, I found that enhanced lipid mixing caused by rat Munc18-1 alone was observed. Biochemistry studies showed that denaturation of rat Munc18-1 or squid …
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Investigating the rate-limiting step of mitochondrial complex I catalysis
… from the point of view of the proton. Using the proteoliposomes system, the pH and solvent isotope dependence of kinetic parameters of purified bovine and *Yarrowia lipolytica* complex I mutants were measured under a range of conditions. I find complex I robustly displays a solvent kinetic …
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Paracoccus denitrificans as a model system for studying the mechanism of respiratory complex I
… characterised and its reconstitution into proteoliposomes was optimised, expanding the toolkit available for the study of complex I variants. The structure of the enzyme was also partially resolved by cryo-EM. The well-resolved map of the hydrophilic domain revealed a novel supernumerary …
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On the mechanisms of transport and energy coupling in ABC exporters
… we also present biochemical experiments with proteoliposomes with a defined phospholipid composition, which suggest that cardiolipin is essential for the transport activity of MsbA. These techniques open the way to further explore lipid-proteins interactions and examine the physiological …
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Molecular mechanisms of snare assembly and expulsion of intravesicular contents in cell secretion
… secretion. Using t- and v-SNARE reconstituted proteoliposomes, and isolated secretory vesicles, the present study was conducted employing various biochemical and biophysical approaches. The results from this work were published in seven research papers, and provide a molecular understanding of …
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The role of mitochondrial transporters in human physiology and adverse drug effects
… their effect on transport was assessed in proteoliposomes with reconstituted hAAC1∆1-10, enabling characterization of their inhibition kinetics. This approach confirmed that chebulinic acid, CD-437 and suramin are potent hAAC1∆1-10 inhibitors with IC<sub>50</sub>-values in the low …
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Functional characterisation of the human mitochondrial ATP-Mg/Pi carriers and their disease-causing variants
… as exhibited by APC1. Activity assays with proteoliposomes showed that APC2 and APC3b also transported adenine nucleotides and were regulated by calcium. However, the transport activities and response to calcium differed between the paralogues. APC2 has a high transport rate and an on/off …
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