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Showing 1 to 20 of 48 for “"proteinase K"”.

  1. Copper/zinc Sods of Salmonella Typhimurium: Enzymatic Properties and Correlation to Pathogenesis

    Krishnakumar, RadhaUniversity of Illinois at Urbana-Champaign2015

    … of the protein that renders SodCI sensitive to proteinase K digestion. This is in sharp contrast to wild-type SodCI, which is resistant to proteinase K. Thus it appears that SodCI must maintain its dimeric conformation, perhaps not for catalytic activity, but possibly to remain tethered within …

    uiuc Repository record

  2. Investigation of the Efficacy of Various Proteinase-Treated "Serpula (Treponema) Hyodysenteriae" Vaccines

    Coyle, Daniel C.Drake University1991

    … to enzymatic digestion of the following proteinases: Proteinase K, proteinase type X-15, proteinase type 2 fungal, papain proteinase, and pepsin proteinase. Sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE) was performed on all bulk samples as well as control samples. …

    drake Repository record

  3. Roles of surface interaction on Cryptosporidium parvum oocyst transport in subsurface environment

    Liu, YuanyuanUniversity of Illinois at Urbana-Champaign2013

    … of untreated oocysts and oocysts modified by proteinase K in RSPF cell were compared. The proteinase K modification resulted in 10 times higher electrophoretic softness and 100 times lower van der Waals interaction of proteinase K modified oocysts than untreated oocysts. Though oocysts …

    uiuc Repository record

  4. Study of zein protein body formation in a heterologous system (<i>Xenopus laevis oocyte</i>)

    Lee, Dong-HeeVirginia Tech1992

    … physical assays of proteolytic digestion (proteinase K) and chemical modification (biotinylation) on isolated protein vesicles from <i>Xenopus</i> oocytes injected with <i>in vitro</i> transcribed 27K zein mRNA. In addition, the transmembrane features were analyzed by monitoring the …

    vt Repository record

  5. Investigation of preferential binding properties of monoclonal antibodies M1:1D2 and H4:4 towards the conformers of recombinant prion protein

    Nainawat, JayantUniversity of Strathclyde2010

    … of brain tissue and western blotting to detect proteinase K resistant prion protein. At present, no diagnostic test exists for the detection of prion diseases in live animals or humans. Use of mAbs will certainly increase sensitivity of diagnosis and it may also allow detection of PrPSc in body …

    strathclyde Repository record

  6. Effects of co-expression of chaperone combinations on production of soluble plasmodial protein PfAdoMetDC in E. coli

    Makhoba, Xolani HenryUniversity of Zululand2015

    … combinations was not completely degraded by proteinase K and its activity was higher compared University of Zululand ii to the protein that was not supplemented with molecular chaperones. Almost similar results were observed on PfAdoMetDC co-expressed with DnaJ+GroEL, combined with the …

    zulu Repository record

  7. In vitro studies on the SecA involvement in the assembly of an integral membrane protein with large hydrophilic domain

    Antonoaea, Raluca

    … of the membrane-bound nascent Momp2 chains with proteinase K yielding two prominent protease-protected fragments of 16 and 26 kDa. Because these fragments were chased to a full-size translocation product of Momp2 after release of the ribosome by puromycin they must represent translocation …

    freiburg-diss Repository record

  8. Hodnocení efektivity vybraných metodických přístupů za účelem disagregace stafylokokové biofilmové biomasy

    Nosková, MartinaUniverzita Karlova, Farmaceutická fakulta v Hradci Králové2022

    … For the biochemical approach was chosen enzyme proteinase K, the impact of sonication, shaking with/without glass beads was studied within the physical approaches. Methods: Staphylococcal biofilm was formed in vitro in two methodical configurations - in the wells of a microtiter plate and on …

    charles-prague Repository record

  9. SENSITIVE AND SPECIFIC DETECTION OF SARS-COV-2 IN SALIVA USING REVERSE TRANSCRIPTASE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

    Juntila, Casey2021

    … Purified SARS-CoV-2 genomic RNA is spiked into Proteinase K and heat-treated saliva from a SARS-CoV-2 negative donor to simulate a positive donor sample used to characterize and optimize this workflow, detecting 200 copies of SARS-CoV-2 genomic RNA by all three gene targets. The utility of …

    nmu Repository record

  10. Development of a T-Loop Assay to Investigate T-Loop Dynamics and Structure

    Mak, Sin Man2016

    … overhang. DNA isolated with typical procedures (Proteinase K at 55°C for 4 hrs, phenol/chloroform extraction) are thus linear and telomeric overhangs are susceptible to digestion by a 3’-5’ exonuclease (ExoI). The “overhangs” in t-loop structures should be resistant to ExoI. If we lower the …

    utswmed Repository record

  11. Development and Optimization of a Food-Grade Antimicrobial by Lactic Acid Bacteria Isolated from Raw Milk

    Ichinomiya, Akimoto2017

    … of the proteolytic enzymes, α- chymotrypsin, proteinase K, trypsin, and pepsin on both N40615 produced antimicrobial and the nisin standard, (Nisaplin™) were similar. Thus, N40615 produced bacteriocin was assumed to be nisin. The antimicrobial activity of N40615 in fermented skim milk was …

    sdstate Repository record

  12. Vergleichende Analyse der Gerstmann-Straeussler-Scheinker-Syndrom-assoziierten Mutation A117V mit der neuen pathogenen Mutation G114V des humanen Prion-Proteins in vivo und in vitro

    Schmalzbauer, RuedigerLudwig-Maximilians-Universität

    … PrP G114V and PrP A117V revealed enhanced proteinase K resistance and increased detergent insolubility. The close similarities displayed by both mutations point to a common pathological mechanism that differs from other prion diseases, thus arguing for a distinct class of disease.

    lmu-germany Repository record

  13. Einfluss der Helix 1 und des β-Faltblattes auf die Aggregation des Prionproteins und seine Amyloidstruktur

    Watzlawik, Jens2012

    … die Helix 1 allerdings nicht resistent gegenüber Proteinase K-Verdau in fibrillärem humPrP23-159, was andeutet, dass Helix 1 nicht in ein β-Faltblatt umgewandelt wird. Dies wird bestätigt durch quantitative FTIR-Spektroskopie, da nahezu kein Unterschied im β-Faltblattgehalt zwischen fibrillärem …

    goettingen Repository record

  14. „Ex vivo” Replikation des pathogenen Prion Proteins

    Heinig, Lars2012

    … Konformationsänderung des PrPC wurde durch den Proteinase K (PK) Verdau im Wb gezeigt. Mit Hilfe der FACS Analyse konnte außerdem die Zunahme des PrP Gehaltes dargestellt werden. Die in dieser Arbeit hergestellten Zelllinien können als neues Tool für die Identifizierung der Interaktionspartner …

    goettingen Repository record

  15. Crystallographic studies on a cold adapted subtilase and proteins involved in mRNA processing

    Arnórsdóttir, Jóhanna2012

    … einer Subtilisin-ähnlichen Serin-Proteinase des psychrotrophen marinen Bakteriums, Vibrio sp. PA-44, wurde durch molekularen Ersatz bei 1,84 Å Auflösung aufgeklärt. Damit konnte zum ersten Mal die Struktur einer kälteadaptiven Subtilase aufgeklärt werden. Dies macht es möglich, die …

    goettingen Repository record

  16. Historical Genetic Analysis Of Horned Lark (Eremophila Alpestris) Specimens From The Prairie Pothole Region

    Cain, Gregory Omar

    … three mechanisms of DNA isolation: EDTA with proteinase K followed by column-based purification, chelating resin with dithiothreitol, and a Direct PCR method without DNA purification. Three fragments of mitochondrial DNA loci were amplified including control region, cytochrome c oxidase I and …

    nodak Repository record

  17. Interaction studies of the cellular prion protein

    Hundt, ChristophLudwig-Maximilians-Universität

    … The protein was expressed as a glycosylated, proteinase K sensitive protein which is transported to the plasma membrane of yeast cells. Recently, the 37-kDa/67-kDa laminin receptor LRP/LR was identified as the receptor for cellular PrP. Besides the in vitro interaction of PrP and LRP the …

    lmu-germany Repository record

  18. Preparation, characterisation and transcriptome analysis of RNA frm human vCJD brains.

    Sherwood, KarenThe University of Edinburgh2008

    … brains which minimized infectivity and reduced Proteinase K resistant prion protein levels to undetectable by biochemical assay was developed. RNA preparations were made from sample of the frontal parasagital cortex, sub-frontal cortex and cerebellum of 78 human autopsy cases; 21 vCJD, 26 other …

    edinburgh Repository record

  19. Emulsion-Templated Porous Polymers as Support Materials for Covalent Enzyme Immobilization

    Kimmins, Scott DavidDurham University2011

    … The enzymes, Lipase from Candida Antarctica and Proteinase K from Tritirachium album were immobilized either directly onto the polyHIPE material or via a hydrophilic spacer group, O,O’-bis(3-aminopropyl)polyethylene glycol. CAL was immobilized with a loading of between 5.4 and 7.5 wt. % per g of …

    durham Repository record

  20. Investigating the Potential Role of α-Synuclein in Tau Aggregation and Toxicity

    Sengupta, UrmiThe University of Texas Medical Branch at Galveston2020

    … in aggregate size, conformation, sensitivity to proteinase K digestion, tryptic digestion and toxicity, suggesting them as distinct α-Syn oligomeric strains. I have also analyzed brain-derived α-Syn oligomers (BDSOs) from AD, DLB and PD brain tissues. I observed that disease associated BDSOs were …

    utmb Repository record

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