Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “"protein-RNA"”.
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Specific Bacteriophage Coat Protein-Rna Interactions
The coat proteins of the group I bacteriophages translationally repress their replicase genes by binding specifically to a region around the initiation codon called the translational operator. A large number of operator variants have been synthesized by using RNA ligase and transcription of …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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Protein-RNA domain-domain interactions in a tRNA sythetase system
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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High-throughput methods to analyze protein-DNA and protein-RNA interactions
… contains the information to make thousands of RNA and protein molecules. However, a cell typically expresses only a fraction of its genes, with different phenotypes arising because of differentially expressed genes. A cell controls its gene expression at different levels. For example, it can …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
"The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Optimizing a protein-RNA aptamer gene regulatory system using an engineered peptide library
… This system, based on the interaction between proteins and proteinbinding RNA aptamers, takes advantage of the reversible interaction between TetR and its RNA aptamer binding partner 5-1.2 to modulate gene expression. With no tetracyclines present, TetR preferentially binds to aptamer 5-1.2 in …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… post-transcriptional processing of pre-mRNA transcripts and export of the correctly processed mRNAs from the nucleus to the cytoplasm, where translation takes place. Evidence has accumulated suggesting that these steps are both functionally and physically coupled by protein-protein …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
<p>In the cell, RNA and protein, interact to form ribonucleoprotein complexes (RNPs) that have vital structural, catalytic and regulatory roles. Despite their functional importance, the mechanistic details and dynamics of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy …
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Kinetics and thermodynamics of protein-RNA interactions and protein folding in vitro and in cells
This Dissertation was approved for publication on 2015-04-20 at 07:49.
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Protein-Protein and Protein-Rna Interactions in the in Vitro Assembly of the R17 Bacteriophage
… R17 bacteriophage is a positive single stranded RNA virus that specifically infects male strains of E. coli. The role of RNA protein interactions in the in vitro assembly of the virus is examined in order to determine both what features of the RNA are important for its selective packaging by R17 …
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The Effect of Infection with Polyoma Virus on the Protein, RNA, and DNA of Mouse Embryo Cell Cultures
… in a button. Later, the buttons were thawed and protein, RNA, and DNA analyses were performed on each sample. The micrograms per million cells of protein. RNA, and DNA were determined for each sample and plotted against time.</p> <p>The amounts of protein and RNA in infected cells showed no …
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DOCKGROUND Protein Docking Benchmark Sets and Assessment Resource
Proteins and nucleic acids are key components of cellular functions. To better understand these functions, it is essential to study protein-protein and protein-nucleic acids interactions at the structural level. Compared to the number of currently known proteins, there are relatively few …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… of computational methodologies for predicting protein-RNA binding affinities and designing RNA sequences with specific functional outcomes. The research introduces PANTHER (Protein-Affinity for Nucleic Target-binding, Hybridization, and Energy Regression), a novel machine learning-based scoring …
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Structure determination of a yeast ribosomal protein L30 and pre-mRNA binding site complex by NMR spectroscopy
The yeast (Saccharomyces cerevisiae) ribosomal protein L30 and its auto-regulatory pre-mRNA binding site provide one of the best examples the critical role of protein-RNA interactions in regulation of RNA processing and control of gene translation. A model system for this interaction, which …
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Molecular Dynamic Simulation of Bio-molecular Dynamics, Folding, and Assembly
… I will discuss recent MD simulation studies of RNA, protein-RNA, and protein-nanoparticle interactions with direct comparisons to experiments whenever possible to validate our approaches.
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Exploring Risk Factors of Alzheimer’s Disease Using Mouse Models
… datasets) to study whether they can mimic protein/RNA expression discrepancies, molecular changes, and enriched pathways found in human AD cases. The mouse models especially 5xFAD and APPNL-G-F show proteomic signatures similar to human AD but lack human-specific AD progressions, such as …
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GPU-Optimized Graph Theory Analysis of Allosteric Protein Signaling Networks
… we performed MD simulations of several protein-RNA complex, where we abstracted the residues and their interactions as nodes and edges, respectively, to characterize the molecular interaction network. We then systematically compare the change in the betweenness upon the deletion of the …
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